STRATEGIES FOR THE LOCALIZATION AND SYNTHESIS OF PROTEIN BINDING SITES

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. CONTENTS VII
4. STRATEGIES FOR THE LOCALIZATION AND SYNTHESIS OF PROTEIN BINDING SITES 1
5. CONTINUOUS FLOW PEPTIDE SYNTHESIS 41
6. SPECIFICITIES OF AUTOANTIBODIES TO SMALL NUCLEAR RIBONUCLEOPROTEINS (U snRNPs) IN SERA FROM PATIENTS WITH CONNECTIVE TISSUE DISEASES 61
7. NEW ULTRASENSITIVE DETECTION SYSTEMS fOR BIOLUMINESCENCEENHANCED ENZYME IMMUNOASSAYS 89
8. PRACTICAL CONSIDERATIONS IN BIOLUMINESCENCE-ENHANCED ENZYME IMMUNOASSAYS ILLUSTRATED BY A NEUTRAL PROTEINASE INHIBITOR OF PORCINE LEUKOCYTES 105
9. A NEW, VERY SENSITIVE, BIOLUMINESCENCE-ENHANCED DETECTON SYSTEM fOR PROTEIN BLOTTING (WESTERN BLOT) 115
10. THE USE OF COLLOIDAL METAL PARTICLES IN PROTEIN BLOTTING 123
11. AMINO-ACID SEQUENCE ANALYSIS OF PROTEINS SEPARATED BY ONE- AND TWO-DIMENSIONAL GEL ELECTROPHORESIS AND ELECTROBLOTTED ON POLYBASE-COATED GLASSFIBER SHEETS 141
12. PHOTOAFFINITY LABELING AND LOCALIZATION BY MICROSEQUENCING OF AN ION CHANNEL PROTEIN 161
13. MICROCHARACTERIZATION OF PHOSPHOSERINE CONTAINING PROTEINSLOCALIZATION OF THE AUTOPHOSPHORYLATION SITES OF SKELETAL MUSCLE PHOSPORYLASE KINASE 185
14. NEW MODULES FOR THE CONSTRUCTION OF A GASPHASE-SEQUENCER 213
15. A NOVEL SOLID SUPPORT FOR GAS-LIQUID MICROSEQUENCING OF PROTEINS AND PEPTIDES - DERIVATIZED POROUS GLASS REPLACES POLYBRENE-COATED GLASFIBER FILTER 219
16. NEW AUTOMATED AMINO ACID ANALYSIS BY HPLC PRECOLUMNS DERIVATISATION WITH FLUORENYLMETHYLOXYCARBONYLCHLORIDE 227
17. HICROCALORIMETRY OF PROTEIN - LIGAND INTERACTION AND PROTEIN UNFOLDING 245
18. APPLICATION OF BIFUNCTIONAL REAGENTS FOR TOPOLOGICAL INVESTIGATION 275
19. TOWARDS THE IDENTIFICATION OF THE NUCLEOTIDE-BINDING SITE OF TUBULIN 299
20. CRYSTALLOGRAPHIC AND IMAGE RECONSTRUCTION STUDIES ON RIBOSOMES 309
21. Cell Culture Techniques for Testing of Biologically Active Peptides and Drugs: Clonogenic Assays Using Agar-Containing Glass Capillaries 335
22. ELECTROTRANSFORMATION OF CULTURE CELLS 359
23. AUTHOR INDEX 377
24. Subject Index 379