AGAROSE ISOELECTRIC FOCUSING OF MONOCLONAL IgM AND IgG ANTIBODIES

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. CONTENTS VII
4. SECTION I. THEORY AND METHODS
5. GEL ELECTROFOCUSING WITH INCREASED DEGREES OF FREEDOM 3
6. pH-MOBILITY CURVES OF PROTEINS BY ISOELECTRIC FOCUSING COMBINED WITH ELECTROPHORESIS AT RIGHT ANGLES 23
7. PARTITIONING AND ELECTROPHORESIS IN FLEXIBLE POLYMER NETWORKS 39
8. DOUBLE ONE-DIMENSIONAL SLAB-GEL ELECTROPHORESIS 53
9. ULTRATHIN-LAYER HORIZONTAL ELECTROPHORESIS, ISOELECTRIC FOCUSING, AND PROTEIN MAPPING IN POLYACRYLAMIDE GELS ON CELLOPHANE 67
10. ULTRATHIN-LAYER ISOELECTRIC FOCUSING IN 50-100 pm POLYACRYLAMIDE GELS ON SILANIZED GLASS PLATES OR POLYESTER FILMS 79
11. ISOELECTRIC FOCUSING OF PROTEINS IN AGAROSE GELS AND DETECTION OF THE PROTEINS BY DIFFERENT STAINING PROCEDURES 95
12. AGAROSE ISOELECTRIC FOCUSING OF MONOCLONAL IgM AND IgG ANTIBODIES 105
13. IMMUNOPEROXIDASE STAINING AND RADIOIMMUNOBINDING OF HUMAN TUMOR MARKERS SEPARATED BY DIRECT TISSUE AGAROSE ISOELECTRIC FOCUSING 117
14. AGAROSE AS SUPPORTING MEDIUM FOR ANALYTICAL ISOELECTRIC SING. DETERMINATION OF THE RECENTLY DISCOVERED SUBTYPES HUMAN SERUM PROTEIN Gc (GROUP SPECIFIC COMPONENT) 123
15. FACTS AND ARTEFACTS IN ISOELECTRIC FOCUSING 129
16. ISOELECTRIC FOCUSING PATTERNS OF SIMIAN VIRUS 40 (SV 40) TUMOR ANTIGEN: VARIOUS ALKYLATION PROCEDURES CAUSE REVERSIBLE ARTIFACTS 141
17. THE ROLE OF SIALIC ACIDS IN THE MICROHETEROGENEITY OF ALPHA ACID GLYCOPROTEIN : STUDY BY ISOELECTRIC FOCUSING AND TITRATION CURVES 151
18. THE EFFECT OF ELECTRODE SOLUTIONS ON THE pH DRIFT IN THIN-LAYER ISOELECTRIC FOCUSING 165
19. QUANTITATIVE IMMUNOELECTROPHORESIS. A SURVEY AND SOME APPLICATIONS. 173
20. AFFINITY ELECTROPHORESIS. QUANTIFICATION AND CHARACTERIZATION OF GLYCOPROTEINS WITH LECTINS 193
21. RECENT ADVANCES IN MICROELECTROPHORESIS 203
22. A SIMPLE, EFFECTIVE AND INEXPENSIVE EQUIPMENT FOR POLYACRYLAMIDE GEL ELECTROPHORESIS IN COOLED MICROCAPILLARIES 219
23. LINEAR POLYACRYLAMIDE GRADIENT GELS FOR MOLECULAR WEIGHT ESTIMATION OF PROTEINS 227
24. SOLUBLE POLYACRYLAMIDE GRADIENT GELS IN THE SEPARATION AND ESTIMATION OF PROTEINS 235
25. QUANTITATION OF PROTEINS SEPARATED BY TWO-DIMENSIONAL PAGE: A NOVEL DYE ELUTION TECHNIQUE COMPARED WITH A DENSITOMETRIC PROCEDURE 241
26. PREPARATION AND ANALYSIS OF PROTEINS AND PROTEIN FRAGMENTS BY TWO-DIMENSIONAL THIN-LAYER GEL FILTRATION AND ISOELECTRIC FOCUSING 249
27. ENHANCEMENT OF LOAD CAPACITY IN ISOTACHOPHORESIS 255
28. A MODEL FOR THE USE OF MORE THAN ONE SINGLE BUFFERING COMMON COUNTERION IN ISOTACHOPHORESIS 265
29. SEPARATION OF MOLECULES IN STEADY STATE ELECTROPHORESIS SYSTEMS WITH ZONES CONTAINING MORE THAN ONE TERMINATING ION 275
30. FLOW-THROUGH CONTINUOUS THIN LAYER CARRIER-FREE ISOTACHOPHORESIS 287
31. SECTION II. HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS
32. TWO-DIMENSIONAL ELECTROPHORESIS: HIGH RESOLUTION OF PROTEINS AND AUTOMATIC EVALUATION OF THE PATTERNS UNDER DIFFERENT METHODICAL CONDITIONS 297
33. HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORETIC MAPPING OF HUMAN PROTEINS 313
34. ESTIMATION OF TWO-DIMENSIONAL ELECTROPHORETIC SPOT INTENSITIES AND POSITIONS BY MODELING 329
35. INTERNAL CHARGE STANDARDIZATION FOR TWO-DIMENSIONAL ELECTROPHORESIS 341
36. A METHOD FOR STUDYING PROTEINS IN 2-D GELS USING THERMAL DENATURATION ANALYSIS 351
37. THE QUANTITATION OF RADIOACTIVELY LABELED PROTEINS ON TWO-DIMENSIONAL GELS: TESTS OF A METHOD FOR ANALYZING CHANGES IN PROTEIN SYNTHESIS AND GENE EXPRESSION 361
38. TWO-DIMENSIONAL ELECTROPHORETIC MAPPING OF HUMAN ERYTHROCYTE PROTEINS 383
39. TWO-DIMENSIONAL ELECTROPHORESIS OF HUMAN SALIVA 395
40. TWO-DIMENSIONAL ELECTROPHORESIS OF HUMAN URINARY PROTEINS IN HEALTH AND DISEASE 405
41. ALTERATIONS OF TWO-DIMENSIONAL ELECTROPHORETIC MAPS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES INDUCED BY CONCANAVALIN A 415
42. HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS (ISO-DALT) IN CLINICAL CHEMISTRY: COMPARISON WITH ROUTINE ELECTROPHORETIC AND IMMUNOLOGICAL METHODS 425
43. TWO DIMENSIONAL MAPS OF HUMAN APOLIPOPROTEINS IN NORMAL AND DISEASED STATES 437
44. ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL ELECTROPHORESIS OF VIRAL PROTEINS AND VIRUS-INFECTED CELLS 475
45. SECTION III. PREPARATIVE SEPARATIONS
46. ISOELECTRIC FOCUSING, ISOELECTRIC POINTS, AND THE PREPARATIVE SEPARATION OF PROTEINS 493
47. PREPARATIVE MULTIPHASIC ZONE ELECTROPHORESIS AND ISOELECTRIC FOCUSING IN DENSITY GRADIENTS USING THE POLY-PREP 503
48. MULTIPHASIC COLUMN ELECTROFOCUSING: AN APPROACH TO COMPOSITE FRACTIONATION PROBLEMS 517
49. PREPARATIVE ELECTROFOCUSING OF ENZYMES INVOLVED IN AROMATIC DEGRADATION IN T. CUTANEUM 529
50. PREPARATIVE FLAT-BED ISOELECTRIC FOCUSING OF LDH, MDH, ALD, SDH AND G-6.PDH FROM RABBIT LENS 539
51. CONTINUOUS-FLOW ISOELECTRIC FOCUSING OF PROTEINS 545
52. RECYCLING OF CARRIER AMPHOLYTES BY ULTRAFILTRATION 555
53. REMOVAL OF SALTS AND CARRIER AMPHOLYTES FROM BILIPEPTIDES OF LOW MOLECULAR WEIGHT 565
54. PREPARATIVE ELECTROPHORESIS IN GEL-BLOCKS WITH DISCONTINUOUS ELUTION 571
55. FURTHER DEVELOPMENTS IN PREPARATIVE DISPLACEMENT ELECTROPHORESIS IN GEL 583
56. VELOCITY GRADIENT STABILISED CONTINUOUS ELECTROPHORESIS 591
57. PREPARATIVE DENSITY GRADIENT ELECTROPHORESIS OF LYMPHOID CELLS 607
58. PREPARATIVE CELL ELECTROPHORESIS IN A STATIC VERTICAL COLUMN STABILIZED WITH A HEAVY WATER GRADIENT 623
59. SECTION IV. BIOMEDICAL AND BIOLOGICAL APPLICATIONS
60. PAST, PRESENT, AND FUTURE USES OF ELECTROPHORESIS IN CLINICAL MEDICINE 631
61. TWO-DIMENSIONAL PROTEIN PATTERNS OF HUMAN BODY FLUIDS OBTAINED UNDER DISSOCIATING AND NON-DISSOCIATING CONDITIONS 647
62. SIMPLIFIED DETERMINATION OF HEMOGLOBIN A1c DIABETIC PATIENTS BY USE OF ELECTROFOCUSING 655
63. A COMPARISON OF ISOELECTRIC FOCUSING AND ELECTROCHROMATOGRAPHY FOR THE SEPARATION AND QUANTIFICATION OF HEMOGLOBIN A1c 663
64. INVESTIGATIONS OF HAEMOGLOBIN A1c ANALOGUES BY ELECTROFOCUSING ON POLYACRYLAMIDE GELS 669
65. ISOELECTRIC ENZYME PATTERN : A NEW CONCEPT FOR TUMOUR CLASSIFICATION 679
66. MONOCYTE-SPECIFIC ISOELECTRIC FOCUSING PATTERN OF LYSOSOMAL ACID ESTERASE : A NEW POSSIBILITY FOR CELL-SPECIFIC IMMUNOHISTOCHEMICAL IDENTIFICATION 683
67. ISOELECTRIC FOCUSING IN CELLULOSE ACETATE MEMBRANE AND FLATBED GRANULATED GEL: APPLICATION TO THE STUDY OF HUMAN ALDEHYDE DEHYDROGENASE ISOZYMES 687
68. CEREBROSPINAL FLUID (CSF) PROTEIN PATTERNS DETERMINED BY ISOELECTRIC FOCUSING IN MULTIPLE SCLEROSIS 695
69. BINDING AFFINITIES BETWEEN VDBP AND VITAMIN D3, 25-(OH)-D3, 24-25-(OH)2-D3 and 1-25-(OH)2-D3 STUDIED BY ELECTROPHORETIC METHODS (PAGE-IE-F, COMBINED IEFELECTROPHORESIS) AND PRINT-IMMUNOFIXATION 701
70. IMMUNOFIXATION AFTER ELECTROFOCUSING : APPLICATION TO THE STUDY OF THE MICROHETEROGENEITY AND POLYMORPHISM OF SERUM PROTEINS 711
71. MICROHETEROGENEITY AND POLYMORPHISM OF THYROXINE-BINDING GLOBULIN: STUDY IN HUMAN SERUM BY RADIOPRINT IMMUNOFIXATION 727
72. TWO DIMENSIONAL IMMUNELECTROPHORESIS OF PLASMA-ANTITHROMBIN III IN THE PRESENCE OF HEPARIN-AGAROSE 733
73. DETERMINATION OF STREPTOKINASE ANTIBODY TITRE IN PLASMA OR SERUM BY AN IMMUNOELECTROPHORETIC METHOD 743
74. TWO DIMENSIONAL IMMÜNOELECTPOPHORESIS APPLIED TO THE SEMI-QUANTITATIVE DETERMINATION OF BENCE JONES IN PROTEIN IN PATHOLOGICAL URINES 753
75. APPLICATION OP PHA IN ELECTROPHORETIC DIFFERENTIATION OF HUMORAL IMMUNOGLOBULINS 761
76. FEASIBILITY OF STUDYING THE FUNCTIONAL NATURE OF HUMAN IgG SUBCLASS RESPONSES BY MEANS OF ANALYTICAL ISOTACHOPHORESIS 765
77. IN VITRO INVESTIGATIONS OF THE GLUCURONIC ACID PATHWAY WITH THE AID OF ANALYTICAL CAPILLARY ISOTACHOPHORESIS 775
78. QUANTITATIVE RESOLUTION OF 1- AND 2-NAPHTHYLGLUCURONIDES FROM SINGLE MIXED ZONES IN ISOTACHOPHEROGRAMS 781
79. A RAPID METHOD FOR QUANTITATIVE DETERMINATION OF URINARY OXALATE BY ANALYTICAL ISOTACHOPHORESIS 791
80. STUDIES ON SERUM CHOLINESTERASE ISOZYMES AND GENETIC VARIANTS IN HUMAN TISSUES USING POLYACRYLAMIDE GEL ELECTROPHORESIS 797
81. MATERNAL PLASMA PROTEIN VARIATIONS DURING NORMAL AND PATHOLOGIC PREGNANCY: A COMPARATIVE STUDY BY MEANS OF SDS-PAA ELECTROPHORESIS AND OSMOMETRIC EVALUATION OF NUMBER AVERAGE MOLECULAR WEIGHT 805
82. SERUM PROTEIN ANALYSIS BY MOLECULAR INTERPRETATION OF CELLULOSE ACETATE ELECTROPHORESIS: EVALUATION OF ITS CLINICAL USEFULNESS 811
83. GENETICAL AND BIOCHEMICAL CHARACTERIZATION OF A DOMINANT MUTATION OF MOUSE LACTATE DEHYDROGENASE 817
84. VERTEBRATE MITOCHONDRIAL 55S RIBOSOMES: COMPARISON OF PROTEIN PATTERNS WITH BACTERIAL AND CYTOPLASMIC R-PROTEINS BY 2-D PAGE 825
85. ISOELECTRIC PEROXIDASE PATTERNS.AS INDICATORS FOR GROWTH AND FOR THE EFFECT OF GROWTH REGULATORS IN TOBACCO TISSUE CULTURES 833
86. STUDY OF HEMOGLOBIN AND A SOLUBLE HEART PEROXIDASE IN LACERTA GALLOTI AND LACERTA STHELINI BY ISOELECTRIC FOCUSING 841
87. AUTHOR INDEX 847
88. SUBJECT INDEX 849
89. Backmatter 860