STUDIES ON LECTIN-CARBOHYDRATE INTERACTIONS USING DIFFERENTIAL SCANNING CALORIMETRY

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. Contents XI
4. Part I. Lectins: Distribution, Isolation, Characterization, and Function
5. LECTINS - AN INTRODUCTION 3
6. LECTINS: SUGAR-SPECIFIC OR RECEPTOR-SPECIFIC PROTEINS? 11
7. AFFIHITINS: COMBINING SITES COffTAIHING PROTEUS 17
8. ISOLATION OF LECTIN-LÏKE SUBSTANCES FROM CONNECTIVE TISSUE AND INVOLVEMENT IN CELL-CELL RECOGNITION AND CELL-MATRIX INTERACTIONS 23
9. RAT PERITONEAL MACROPHAGES EXPRESS TWO DIFFERENT LECTIN-LIKE RECEPTORS ON THE CELL SURFACE 33
10. HOST-MICROORGANISM INTERACTION: DEVELOPMENTALLY REGULATED LECTIN-MEDIATED CAPTURE OF NEMATODES BY NEMATODE-TRAPPING FUNGI 43
11. SEXUAL CELL RECOGNITION IN CHLAMYDOMONAS EUGAMETOS 51
12. THE LECTINS OF CLOSTRIDIUM BOTULINUM AND THEIR RELATIONSHIP TO TOXIN SPECIFICITY 59
13. ON THE SPECIFICITY AND HYDROPHOBICITY OF LECTINS 73
14. ENZYMIC PHYTOHEMAGGLUTININS : THEIR RELATION TO "CLASSIC" LEGUME P H Y T O H E M A G G L U T I N I NS 81
15. A COMPARISON OF THE TWO LECTINS FROM VICIA CRACCA 93
16. PURIFICATION AND CHARACTERIZATION OF VICIA GRAMINEA LECTIN. INTERACTION OF LABELLED LECTIN WITH NATIVE AND ENZYME-MODIFIED HUMAN M AND N ERYTHROCYTES 101
17. STUDIES OF THE LATHYRUS ODORATUS (LATH) LECTIN 111
18. MISTLETOE LECTINS: CHEMICAL MODIFICATION AND CARBOHYDRATE INTERACTION OF THE D-GALACTOSE SPECIFIC LECTIN I 115
19. MITOGENIC LECTINS OCCUR ONLY IN FEW SEED SAMPLES OF VIGNA (COW PEA) 125
20. LECTIN BINDING PROTEINS FROM PLANTS 135
21. INTERACTIONS BETWEEN SEED PROTEINS AND THEIR RESPECTIVE LECTINS 145
22. SEED LECTINS FROM 4 LINES OF PEA 151
23. ACTIVATION OF ACID PHOSPHATASE FROM POA PRATENSIS BY BINDING TO LECTINS 157
24. EFFECTS OF LECTINS ON ENZYMATIC PROPERTIES PLANT ACID PHOSPHATASES AND RIBONUCLEASES 169
25. ISOLATION OP LECTINS WITH DIFFERENT SPECIFICITIES USING IMMOBILIZED IMMUNOGLOBULIN 179
26. Part II. Methods Based on Reactions of Lectins
27. LECTIN BINDING MEASURED BY FLUORESCEIN FLUORESCENCE POLARIZATION (FFP) TECHNIQUES 187
28. STUDIES ON LECTIN-CARBOHYDRATE INTERACTIONS USING DIFFERENTIAL SCANNING CALORIMETRY 199
29. BIOCHEMICAL ANALYSIS BASED ON THE USE OF IMMOBILIZED LECTINS PLACED IN CONTINUOUS FLOW SYSTEMS 207
30. LECTINS AS PROBES FOR MEMBRANE ASYMMETRY AND COMPARTMENTALIZATION OF SACCHARIDE MOIETIES IN CELLS 215
31. STRUCTURAL BASIS FOR THE AFFINITY OF FOUR INSOLUBILIZED LECTINS, WITH A SPECIFICITY FOR ct-D-MANNOSE, TOWARDS VARIOUS GLYCOPEPTIDES WITH THE N-GLYCOSYLAMINE LINKAGE AND RELATED OLIGOSACCHARIDES 221
32. INTERACTIONS OF PHA WITH HUMAN NORMAL SERUM PROTEINS 231
33. PARAMETERS OF ELECTROPHORETIC DETERMINATION OF DISSOCIATION CONSTANTS BETWEEN GLYCOPROTEINS AND LECTINS 241
34. AN AUTORADIOGRAPHICAL APPROACH TO THE STUDY OF BINDING OF LECTINS AND THEIR CORRESPONDING ANTIBODIES TO GLYCOPROTEINS IN CROSSED IMMUNOELECTROPHORESIS 259
35. Part III. Glycoprotein studied by Reactions with Lectins
36. STUDY OF THE MOLECULAR HETEROGENEITY OF ALPHA-FETOPROTEIN BY LECTIN-AFFINITY IMMUNOELECTROPHORESIS 271
37. CARBOHYDRATE MICROHETEROGENEITY OF HUMAN ALPHA-FETOPROTEIN. ONCODEVELOPMENTAL ASPECTS 283
38. THE DIAGNOSTIC SIGNIFICANCE OF CONCANAVALIN A REACTIVITY PATTERN OF HUMAN ALPHA-FETOPROTEIN IN AMNIOTIC FLUID 293
39. APPLICATION OF LECTIN AFFINITY ELECTROPHORESIS FOR STUDIES OF MICROHETEROGENEITY OF HUMAN ALPHAFETOPROTEIN 303
40. THREE-DIMENSIONAL AFFINITY ELECTROPHORESIS OF HUMAN ALPHAFETO-PROTEIN 315
41. H U R I N E ALPHA-FETOPROTEIN - DEMONSTRATION AND DYNAMICS OF FOUR MOLECULAR FORMS IN CONCANAVALIN A CROSSED AFFINO-IMMUNOELECTROPHORESIS 327
42. THE DETECTION OF HORMONE-ASSOCIATED VARIATIONS IN a ALPHA-ACID GLYCOPROTEIN USING CONCANAVALIN A CROSSED IMMUNO-AFFINOELECTROPHORESIS 339
43. IDENTIFICATION AND CHARACTERIZATION OF PLATELET GLYCOPROTEINS BY CROSSED AFFINITY IMMUNOELECTROPHORESIS 347
44. LECTINS AND THE RECEPTOR FOR EPIDERMAL GROWTH FACTOR 355
45. IDENTIFICATION OF HUMAN B-LYMPHOCYTE HISTOCOMPATIBILITY ANTIGEN HLA-DR IN CROSSED IMMUNOELECTROPHORESIS OP TRITON X-lOO SOLUBILIZED LYMPHOCYTE MATERIAL 363
46. LECTIN BINDING PROPERTIES OF CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX CODED LYMPHOCYTE PLASMA MEMBRANE GLYCOPROTEINS 375
47. SEQUENTIAL LECTIN AFFINITY CHROMATOGRAPHY OF SOLUBLE CHICKEN LYMPHOCYTE PLASMA MEMBRANE PROTEINS AND EVALUATION OF SPECIFICITY OF RABBIT ANTISERA AGAINST THE LECTIN BINDING GLYCOPROTEINS 387
48. AFFINITY OF MEASLES VIRUS PROTEINS TO CONCANAVALIN A AS EVALUATED BY CROSSED IMMUNOELECTROPHORESIS 395
49. ISOLATION OF ANTIGENIC SUBUNITS FROM BOVINE VIRAL DIARRHOEA VIRUS BY MEANS OF IMMDBILISED CROTALARIA JUNCEA LECTIN 401
50. RAPID CHANGES IN THE AFFINITY BETWEEN CON-A-SEPHAROSE AND LYSOSOMAL ENZYMES OF HUMAN SALIVA DURING THE DAY 407
51. AUTHOR INDEX 409
52. INDEX 411
53. Backmatter 415