ENHANCEMENT OF COMPLEX PATTERNS IN ISOELECTRIC FOCUSING WITH IMPROVED ISOGEL PERFORMANCE

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. CONTENTS VII
4. SECTION I. THEORY AND METHODS
5. ON THE PORE SIZE AND SHAPE OF HYDROPHILLIC GELS FOR ELECTROPHORETIC ANALYSIS 1
6. GENERALIZATION OF THE DEBYE-HUCKEL THEORY TO MACROION SOLUTIONS OF FINITE SIZE AND CONCENTRATION AS APPLIED TO THEIR ELECTROPHORETIC MOBILITY AND TRANSLATIONAL DIFFUSION COEFFICIENT 17
7. CALCULATION OF THE THERMODYNAMIC CONSTANTS OF CONCANAVALIN A - CARBOHYDRATE INTERACTIONS BY MEANS OF AFFINITY ELECTROPHORESIS 33
8. HIGH RESOLUTION ELECTROPHORESIS OF PROTEINS IN SDS POLYACRYLAMIDE GELS 41
9. CONVENIENT PROCEDURES FOR SDS AND CONVENTIONAL DISC ELECTROPHORESIS 49
10. THE DESIGN AND APPLICATIONS OF A UNIVERSAL GEL ELECTROPHORESIS APPARATUS 65
11. A SIMPLE METHOD FOR CASTING POLYACRYLAMIDE GELS OF VARIOUS THICKNESS AND SIZE FOR FLAT-BED ISOELECTRIC FOCUSING 73
12. ISOELECTRIC FOCUSING ON CELLULOSIC MEMBRANES 77
13. A METHOD FOR PHENOTYPING OF ALPHA-1-ANTITRYPSIN VARIANTS USING SEPARATOR ISOELECTRIC FOCUSING ON AGAROSE 83
14. QUANTIFICATION OF PROTEINS WITH ZONE IMMUNOELECTROPHORESIS 89
15. SPECIFIC AND EFFICIENT PROCEDURES FOR QUANTIFICATION OF PROTEINS SEPARATED IN GEL ELECTROPHORETIC METHODS USING ZONE IMMUNOELECTROPHORETIC ASSAY (ZIA) 103
16. A SIMPLIFIED POLYACRYLAMIDE DENSITY GRADIENT GEL ELECTROPHORESIS WITH A NEW STAINING METHOD, ELECTROSTAINING 117
17. ENHANCEMENT METHODS IN THE LOCALIZATION OF PROTEINS FOLLOWING ELECTROPHORESIS OR ISOELECTRIC FOCUSING 127
18. ONE AND TWO-DIMENSIONAL MICROELECTROPHORESIS AND STAINING OF PROTEINS WITH A SILVER METHOD 133
19. "COLD FOCUS" ISOELECTRIC FOCUSING OF SMALL SAMPLES OF CEREBROSPINAL FLUID 149
20. A UNIQUE SILVER STAINING PROCEDURE FOR COLOUR CHARACTERIZSATION OF POLYPEPTIDES 155
21. AFFINITY-IMMUNODELETION (AID) ISOELECTRIC FOCUSING ON ULTRATHIN GELS APPLIED TO THE IDENTIFICATION OF SWEAT, SALIVA AND BLOOD PROTEINS USING SILVER-DIAMINE STAINING 167
22. ULTRATHIN LAYER ISOELECTRIC FOCUSING IN 20-50 um POLYACRYLAMIDE GELS: COMPARISON OF CONVENTIONAL AND MINIATURE SYSTEMS 181
23. ULTRATHIN LAYER PAGIF: A COST BENEFIT ANALYSIS OF THE FOCUSING DISTANCE 191
24. ISOELECTRIC FOCUSING IN AGAROSE GELS 205
25. SEAPREP^TM 15/45.. A NEW AGAROSE WITH LOW GELLING AND REMELTING PROPERTIES FOR PREPARATIVE ELECTROPHORESIS 213
26. AGAROSE GEL ELECTROENDOSMOSIS: ITS ENHANCEMENT, REDUCTION AND PRACTICAL RAMIFICATIONS 219
27. ENHANCEMENT OF COMPLEX PATTERNS IN ISOELECTRIC FOCUSING WITH IMPROVED ISOGEL PERFORMANCE 229
28. THE SIEVING OF VIRUSES AND VIRAL CAPSIDS DURING AGAROSE GEL ELECTROPHORESIS 237
29. NEW ELECTROPHORETIC TECHNIQUES FOR THE SEPARATION AND CHARACTERIZATION OF POLIO VIRUS PROTEINS 245
30. SECTION II. HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS
31. SDS-GEL GRADIENT ELECTROPHORESIS, ISOELECTRIC FOCUSING AND HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS IN HORIZONTAL, ULTRATHIN-LAYER POLYACRYLAMIDE GELS 259
32. TWO-DIMENSIONAL ELECTROPHORESIS ON CELLULOSE ACETATE MEMBRANES 271
33. ISOELECTRIC FOCUSING, SDS GEL GRADIENT ELECTROPHORESIS AND TWO-DIMENSIONAL ELECTROPHORESIS OF TROPICAL AND EUROPEAN LEGUME PROTEINS IN ULTRATHIN POLYACRYLAMIDE LAYERS 281
34. HIGH RESOLUTION POLYPEPTIDE MAPPING OF HUMAN HAIR ROOTS FROM HEALTHY INDIVIDUALS AND PATIENTS WITH GENETIC DEFECTS 289
35. ANALYSIS OF CULTURED SKIN FIBROBLASTS FROM PATIENTS WITH DUCHENNE MUSCULAR DYSTROPHY USING ELECTROPHORETIC TECHNIQUES 295
36. STUDIES OF GENE EXPRESSION IN HUMAN LYMPHOCYTES USING HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS 309
37. ISOELECTRIC FOCUSING AND TWO DIMENSIONAL MAPS OF NORMAL AND CYSTIC FIBROSIS SALIVA 317
38. ANALYSIS OF HUMAN AMNIOTIC FLUID PROTEINS BY TWODIMENSIONAL ELECTROPHORESIS 329
39. QUANTITATIVE TWO-DIMENSIONAL ELECTROPHORESIS AS A SCREEN FOR GENETIC DISEASE MARKERS 343
40. A QUANTITATIVE TWO-DIMENSIONAL ELECTROPHORETIC SURVEY OF PROTEINS AFFECTED BY CHROMOSOME 21 355
41. MULTISPECTRAL DIGITAL IMAGE ANALYSIS OF COLOR TWODIMENSIONAL ELECTROPHORETOGRAMS 371
42. A COMPUTERIZED SYSTEM FOR MATCHING AND STRETCHING TWO-DIMENSIONAL GEL PATTERNS REPRESENTED BY PARAMETER LISTS 383
43. GELLAB: MULTIPLE 2D ELECTROPHORETIC GEL ANALYSIS 401
44. IMPLICIT MODELING OF SPOTS FOR THE EVALUATION OF TWO-DIMENSIONAL ELECTROPHORETOGRAMS 413
45. SECTION III. BIOMEDICAL AND BIOLOGICAL APPLICATIONS
46. EVALUATION OF IMMUNOGLOBULIN DIVERSITY 427
47. STUDIES OF ANTIBODY CLONOTYPE PATTERNS IN RABBIT ANTIMICROCOCCAL SERA BY ANALYTICAL ISOELECTRIC FOCUSING IN AGAROSE GELS 433
48. MONOCLONAL GAMMOPATHY WITH FREE LIGHT CHAINS 439
49. ISOELECTRIC FOCUSING OF IMMUNOGLOBULINS IN ULTRATHIN LAYERS OF AGAROSE 443
50. ELECTROPHORETIC ABNORMALITIES OF HIGH DENSITY LIPOPROTEINS IN LIVER DISEASES 447
51. SDS-PAGE OF URINARY PROTEINS: CORRELATION OF DIFFERENT PROTEINS TO RENAL CLEARANCE AND TO RENAL AND EXTRARENAL DISEASES 453
52. HEPATIC IMPLICATIONS IN THE METABOLISM OF HUMAN SALIVARY AMYLASE ISOENZYME 463
53. POSTTRANSLATIONAL MODIFICATION OF PANCREATIC AMYLASE IN ACUTE PANCREATITIS: POSSIBLE ROLE OF PANCREATIC DEAMIDASE 471
54. EFFECTS OF DIALYZABLE LEUKOCYTE EXTRACTS WITH TRANSFER FACTOR ACTIVITY ON LEUKOCYTE MIGRATION IN VITRO: VI. STUDIES ON THE PRIMARY STRUCTURE OF TRANSFER FACTOR 479
55. CHARACTERIZATION OF HORSE PLASMA PROTEINS BY SPECTROPHOTOMETRY AND ISOELECTRIC FOCUSING 487
56. ALPHA-1-ANTITRYPSIN DEFICIENCY AND DISEASE 495
57. RARE TYPES OF ALPHA-1-ANTITRYPSIN ASSOCIATED WITH DEFICIENCY 505
58. ISOLATION AND CHARACTERIZATION OF AN ALPHA-1"ANTITRYPSIN -RELATED GLYCOPROTEIN FROM HUMAN LIVER 511
59. CK-MB BY AN IMPROVED ELECTROPHORESIS METHOD 523
60. DETECTION AND CHARACTERIZATION OF CYSTIC FIBROSIS PROTEIN EMPLOYING ISOELECTRIC FOCUSING AND IMMUNOELECTROPHORETIC TECHNIQUES 529
61. IMMUNOFIXATION-AGAROSE ISOELECTRIC FOCUSING TECHNIQUES FOR SCREENING GLIAL TUMOR CELL CULTURES FOR GLIAL MARKERS 537
62. AGAROSE ISOELECTRIC FOCUSING AND RELATED IMMUNOCHEMICAL TECHNIQUES IN THE ANALYSIS OF SERUM GC-GLOBULIN 549
63. ISOELECTRIC FOCUSING IN FORENSIC SEROLOGY 563
64. ISOELECTRIC FOCUSING OF THE COMMON TRANSFERRIN0 ALLELE SUBTYPES 573
65. ISOELECTRIC FOCUSING OF HAIR PROTEINS 585
66. ISOENZYME TYPING OF DRIED BLOODSTAINS USING MYLAR BACKED CELLULOSE ACETATE MEMBRANES 591
67. CHANGES IN THE ELECTROPHORETIC PATTERNS OF STRUCTURAL PROTEINS AND ENZYMES OF HALIBUT (HIPPOGLOSSUS HIPPOGLOSSUS L.) MUSCLE AFTER PROLONGED (TWENTY-FIVE YEARS" FROZEN STORAGE 599
68. ELECTROPHORETIC BEHAVIOR OF CROSSLINKED DNA 607
69. ELECTROPHORETIC MIGRATION OF FORM IV-DNA AND TOPOISOMERASE MONITORING 611
70. PROPERTIES OF NUCLEIC ACIDS PURIFIED FROM AGAROSE 617
71. FRACTIONATION OF CONCATEMERIC DNA OF BACTERIOPHAGE T7 BY AGAROSE GEL ELECTROPHORESIS 627
72. DNA SEQUENCING ON VERY THIN (0,2mm) GELS 635
73. THE DETECTION OF HUMAN INTRACELLULAR DEXOXYRIBONUCLEASE ACTIVITIES USING ONE DIMENSIONAL POLYNUCLEOTIDE— POLYACRYLAMIDE GEL ELECTROPHORESIS 647
74. RECENT DEVELOPMENTS IN TITRATION CURVES OF PROTEINS BY ISOELECTRIC FOCUSING-ELECTROPHORESIS 655
75. A STUDY OF THE STRUCTURAL CONVERSIONS OF TWO CARBOXYL PROTEINASES EMPLOYING ELECTROPHORESIS ACROSS A pH GRADIENT 667
76. ELASTASE DETECTION IN ELECTROPHORETOGRAMS OF COMPLEX ANTIGEN MIXTURES 677
77. POSITIVE PERIODIC ACID-SCHIFF STAINING OF PROTEOLYTIC ENZYMES FOLLOWING ZONE ELECTROPHORESIS IN POLYACRYLAMIDE GEL 685
78. ELECTROPHORETIC AND STAIN METHOD FOR PYRUVATE KINASE ISOENZYME PATTERNS 693
79. ISOENZYMES OF SUPEROXIDE DISMUTASE IN SERRATIA MARCESCENS 699
80. ISOENZYMES OF SUPEROXIDE DISMUTASE IN LIVER FROM LACERTA STEHLINII 703
81. ELECTROPHORETIC MULTIPLICITIES OF γ-GLUTAMYLTRANSFERASES IN HUMAN SERUM AND THEIR CHARACTERIZATION BY AFFINITY CHROMATOGRAPHY 709
82. ISOAMYLASE BY ISOELECTRIC FOCUSING ON MODIFIED CELLULOSE ACETATE 715
83. MULTIPLE FORMS OF TREHALASE FROM AGING SOROCARPS OF THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM 721
84. PURIFICATION OF GRANULOCYTE COLONY STIMULATING FACTOR MONITORED BY ULTRATHIN LAYER ISOELECTRIC FOCUSING 729
85. PROTEOLYTIC DIGESTION OF THE M PROTEIN OF INFLUENZA A VIRUSES AND ISOELECTRIC FOCUSING OF ITS PEPTIDES 735
86. SECTION IV. ISOTACHOPHORESIS AND FREE FLOW ELECTROPHORESIS
87. QUANTITATIVE ANALYSIS IN ISOTACHOPHORESIS 743
88. THE ANALYSIS OF HUMAN SERUM PROTEINS BY CAPILLARY ISOTACHOPHORESIS 753
89. ISOTACHOPHORETIC ASSESSMENT OF ENZYME IMMUNOGLOBULIN CONJUGATES USED IN ENZYME IMMUNOASSAY 767
90. ANALYTICAL CAPILLARY ISOTACHOPHORESIS APPLIED TO THE STUDY OF NUCLEOTIDE-DEPENDENT ENZYMATIC PROCESSES 781
91. CONDITIONS FOR THE SEPARATION OF ADENINE NUCLEOTIDES AND THEIR 3 ^-PHOSPHATE DERIVATIVES IN ANALYTICAL CAPILLARY ISOTACHOPHORESIS 797
92. ISOTACHOPHORETIC ANALYSIS OF HIGH DENSITY LIPOPROTEINS 809
93. CAPILLARY ISOTACHOPHORESIS AS AN ANALYTICAL MONITOR DURING THE SYNTHESIS OF THE C-TERMINAL PENTAPEPTIDE OF BOMBININ 821
94. AN ALTERNATIVE OUTLOOK ON ELECTROKINETIC CELL SEPARATIONS 827
95. CHARACTERIZATION OF THE SURFACE PHENOTYPE OF ELECTROPHORECTICALLY FRACTIONATED MOUSE LYMPHOCYTES BY FLOW MICROFLUOREMETRY ANALYSIS 831
96. EVALUATION OF THE ELECTROPHORETIC SEPARABILITY OF TRYPANOSOMA CRUZI PARASITE STAGES 841
97. SEPARATION AND CHARACTERIZATION OF HUMAN BONE MARROW CELLS BY DENSITY GRADIENT ELECTROPHORESIS 851
98. FREE FLOW ELECTROPHORESIS IN MALARIA RESEARCH 859
99. LIBERATION OF INTRAERYTHROCYTIC PARASITES (PLASMODIUM VINCKEI, PLASMODIUM BERGHEI) FROM ERYTHROCYTES AND ELECTROPHORETIC SEPARATION OF FREE PARASITES FROM HOST CELL CONSTITUENTS 860
100. FREE-FLOW ELECTROPHORESIS SEPARATIONS OF FREE PARASITES (PLASMODIUM FALCIPARUM)ACCORDING TO STAGES 862
101. FREE-FLOW ELECTROPHORESIS ISOLATION OF WHOLE BODY PLASMODIUM BERGHEI SPOROZOITES 866
102. SEPARATION OF FUNCTIONING MAMMALIAN CELLS BY DENSITYGRADIENT ELECTROPHORESIS 871
103. REVIEW OF THE NASA ELECTROPHORESIS PROGRAM 883
104. NUMERICAL ANALYSIS OF CONTINUOUS FLOW ELECTROPHORESIS 899
105. HIGH-RESOLUTION CONTINUOUS-FLOW ELECTROPHORESIS IN THE REDUCED GRAVITY ENVIRONMENT 919
106. APPLICATION OF THE AUTOMATED ELECTROPHORESIS MICROSCOPE SYSTEM TO VARIOUS BIOLOGICAL TESTS 933
107. OPERATIONAL PARAMETERS FOR CONTINUOUS FLOW ELECTROPHORESIS OF CELLS 947
108. REDUCTION OF DROPLET FORMATION AND SEDIMENTATION OF FIXED ERYTHROCYTES IN STATIONARY AND FLOWING SYSTEMS 961
109. DESIGN CONSIDERATIONS OF A THERMALLY STABILIZED CONTINUOUS FLOW ELECTROPHORESIS CHAMBER 971
110. THE SHEEP ERYTHROCYTES ELECTROPHORETIC MOBILITY: EFFECT OF SUPERNATANTS OF LYMPHOCYTES STIMULATED WITH VARIOUS IMMUNOPOTENTIATORS 983
111. ELECTRICAL INSULATION OF ELECTROPHORETIC FLOWS AND A "RAIN BOX" 995
112. Author Index 1005
113. Subject Index 1009
114. Backmatter 1022