11 Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite
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Yandri Yandri
Abstract
The stability of enzymes which play an important role as biocatalysts in many industrial processes is a persistent challenge with significant impact on production costs. In this study, improvement of the stability of α-amylase obtained from Aspergillus fumigatus was attempted by immobilizing the enzyme onto zeolite using adsorption method. For purification, the isolated enzyme was subsequently subjected to centrifugation, fractionation, and dialysis. The native enzyme was found to have an optimum temperature of 50 °C, while the immobilized enzyme, the optimum temperature of 60 °C was found. The immobilized enzyme was found to have the KM value of 11.685 ± 0.183mg mL−1 substrate and Vmax of 1.406 ± 0.049 μmol mL−1 min−1, while for the native enzyme, the KM value of 3.478 ± 0.271 mg mL−1 substrate and the Vmax of 2.211 ± 0.096 μmolmL−1 min−1 were obtained. Furthermore, the immobilized enzyme displays the ΔGi of 106.76 ± 0.00 kJ mol−1 and t½ of 90.40 ± 0.00 min, while the native enzyme, the values obtained are ΔGi of 104.35 ± 1.09 kJ mol−1 and t½ of 38.75 ± 1.53 min. As can be seen, the t½ of immobilized enzyme is 2.38 times longer than that of native enzyme, justifying a very significant stability enhancement of the enzyme as a result of. Another important finding is that the immobilized α-amylase enzymewas able to retain its activity as high as 13.80 ± 1.19%activity after five cycles, indicating its potential for industrial use.
Abstract
The stability of enzymes which play an important role as biocatalysts in many industrial processes is a persistent challenge with significant impact on production costs. In this study, improvement of the stability of α-amylase obtained from Aspergillus fumigatus was attempted by immobilizing the enzyme onto zeolite using adsorption method. For purification, the isolated enzyme was subsequently subjected to centrifugation, fractionation, and dialysis. The native enzyme was found to have an optimum temperature of 50 °C, while the immobilized enzyme, the optimum temperature of 60 °C was found. The immobilized enzyme was found to have the KM value of 11.685 ± 0.183mg mL−1 substrate and Vmax of 1.406 ± 0.049 μmol mL−1 min−1, while for the native enzyme, the KM value of 3.478 ± 0.271 mg mL−1 substrate and the Vmax of 2.211 ± 0.096 μmolmL−1 min−1 were obtained. Furthermore, the immobilized enzyme displays the ΔGi of 106.76 ± 0.00 kJ mol−1 and t½ of 90.40 ± 0.00 min, while the native enzyme, the values obtained are ΔGi of 104.35 ± 1.09 kJ mol−1 and t½ of 38.75 ± 1.53 min. As can be seen, the t½ of immobilized enzyme is 2.38 times longer than that of native enzyme, justifying a very significant stability enhancement of the enzyme as a result of. Another important finding is that the immobilized α-amylase enzymewas able to retain its activity as high as 13.80 ± 1.19%activity after five cycles, indicating its potential for industrial use.
Chapters in this book
- Frontmatter I
- Preface of the Book of Proceedings of the Virtual Conference on Chemistry and its Applications (VCCA-2022). V
- Contents VII
- List of contributing authors XIX
- 1 Dipeptidyl peptidase IV: a multifunctional enzyme with implications in several pathologies including cancer 1
- 2 A mini review on the prospects of Fagara zanthoxyloides extract based composites: a remedy for COVID-19 and associated replica? 57
- 3 Triterpenoids of antibacterial extracts from the leaves of Bersama abyssinica Fresen (Francoaceae) 75
- 4 Physicochemical assessment and insilico studies on the interaction of 5-HT2c receptor with herbal medication bioactive compounds used in the treatment of premature ejaculation 87
- 5 Xanthoangelol, geranilated chalcone compound, isolation from pudau leaves (Artocarpus kemando Miq.) as antibacterial and anticancer 121
- 6 Exploration of bioactive compounds from Mangifera indica (Mango) as probable inhibitors of thymidylate synthase and nuclear factor kappa-B (NF-Κb) in colorectal cancer management 137
- 7 Identification of potential inhibitors of thymidylate synthase (TS) (PDB ID: 6QXH) and nuclear factor kappa-B (NF–κB) (PDB ID: 1A3Q) from Capsicum annuum (bell pepper) towards the development of new therapeutic drugs against colorectal cancer (CRC) 165
- 8 Synthesis, characterization and in vitro activity study of some organotin(IV) carboxylates against leukemia cancer cell, L-1210 199
- 9 Phytochemicals from Annona muricata (Sour Sop) as potential inhibitors of SARS-CoV-2 main protease (Mpro) and spike receptor protein: a structure-based drug design studies and chemoinformatics analyses 207
- 10 Identification of novel inhibitors of P13K/AKT pathways: an integrated in-silico study towards the development of a new therapeutic agent against ovarian cancer 231
- 11 Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite 261
- 12 Phytochemical components and GC–MS analysis of Petiveria alliaceae L. fractions and volatile oils 273
- 13 Characterization of crude saponins from stem bark extract of Parinari curatellifolia and evaluation of its antioxidant and antibacterial activities 287
- 14 Physicochemical and free radical scavenging activity of Adansonia digitata seed oil 307
- 15 Photoprotection strategies with antioxidant extracts: a new vision 321
- 16 A systematic DFT study of arsenic doped iron cluster AsFen (n = 1–4) 335
- 17 Effect of case-based learning, team-based learning and regular teaching methods on secondary school students’ self-concept in chemistry in Maara sub-county, Tharaka Nithi county, Kenya 345
- 18 Random and block architectures of N-arylitaconimide monomers with methyl methacrylate 359
- 19 Evaluation of phytochemicals and amino acid profiles of four vegetables grown on a glyphosate contaminated soil in Southwestern Nigeria 399
- Index 411
Chapters in this book
- Frontmatter I
- Preface of the Book of Proceedings of the Virtual Conference on Chemistry and its Applications (VCCA-2022). V
- Contents VII
- List of contributing authors XIX
- 1 Dipeptidyl peptidase IV: a multifunctional enzyme with implications in several pathologies including cancer 1
- 2 A mini review on the prospects of Fagara zanthoxyloides extract based composites: a remedy for COVID-19 and associated replica? 57
- 3 Triterpenoids of antibacterial extracts from the leaves of Bersama abyssinica Fresen (Francoaceae) 75
- 4 Physicochemical assessment and insilico studies on the interaction of 5-HT2c receptor with herbal medication bioactive compounds used in the treatment of premature ejaculation 87
- 5 Xanthoangelol, geranilated chalcone compound, isolation from pudau leaves (Artocarpus kemando Miq.) as antibacterial and anticancer 121
- 6 Exploration of bioactive compounds from Mangifera indica (Mango) as probable inhibitors of thymidylate synthase and nuclear factor kappa-B (NF-Κb) in colorectal cancer management 137
- 7 Identification of potential inhibitors of thymidylate synthase (TS) (PDB ID: 6QXH) and nuclear factor kappa-B (NF–κB) (PDB ID: 1A3Q) from Capsicum annuum (bell pepper) towards the development of new therapeutic drugs against colorectal cancer (CRC) 165
- 8 Synthesis, characterization and in vitro activity study of some organotin(IV) carboxylates against leukemia cancer cell, L-1210 199
- 9 Phytochemicals from Annona muricata (Sour Sop) as potential inhibitors of SARS-CoV-2 main protease (Mpro) and spike receptor protein: a structure-based drug design studies and chemoinformatics analyses 207
- 10 Identification of novel inhibitors of P13K/AKT pathways: an integrated in-silico study towards the development of a new therapeutic agent against ovarian cancer 231
- 11 Immobilization of α-amylase from Aspergillus fumigatus using adsorption method onto zeolite 261
- 12 Phytochemical components and GC–MS analysis of Petiveria alliaceae L. fractions and volatile oils 273
- 13 Characterization of crude saponins from stem bark extract of Parinari curatellifolia and evaluation of its antioxidant and antibacterial activities 287
- 14 Physicochemical and free radical scavenging activity of Adansonia digitata seed oil 307
- 15 Photoprotection strategies with antioxidant extracts: a new vision 321
- 16 A systematic DFT study of arsenic doped iron cluster AsFen (n = 1–4) 335
- 17 Effect of case-based learning, team-based learning and regular teaching methods on secondary school students’ self-concept in chemistry in Maara sub-county, Tharaka Nithi county, Kenya 345
- 18 Random and block architectures of N-arylitaconimide monomers with methyl methacrylate 359
- 19 Evaluation of phytochemicals and amino acid profiles of four vegetables grown on a glyphosate contaminated soil in Southwestern Nigeria 399
- Index 411
