THE DETERMINATION OF PTERINS, BIOGENIC AMINE METABOLITES, AND AROMATIC AMINO ACIDS IN CEREBROSPINAL FLUID USING ISOCRATIC REVERSE PHASE LIQUID CHROMATOGRAPHY WITH IN SERIES DUAL CELL COULOMETRIC ELECTROCHEMICAL AND FLUORESCENCE DETECTION:- USE IN THE STUDY OF INBORN ERRORS OF DIHYDROPTERIDINE REDUCTASE AND 5,lO-METHYLENETETRAHYDROFOLATE REDUCTASE

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. LIST OF PARTICIPANTS VII
4. CONTENTS XIII
5. 1. CHEMISTRY AND ANALYSIS OF PTERIDINES
6. ELECTROLYSIS OF PTERIDINES 3
7. NITRITE OXIDATION OF TETRAHYDROPTERINS 17
8. ACYLATION OF TETRAHYDROPTERINS 27
9. SYNTHESIS AND PROPERTIES OF 5,6-DIHYDROPTERIDINES 33
10. SYNTHESIS AND PROPERTIES OF NEW TETRAHYDROPTERIDINES 45
11. INTER- AND INTRAMOLECULAR REDOX-REACTIONS BY 5,6- RESP. 7,8-DIHYDROPTERINES; DO THESE COMPOUNDS BEHAVE AS REDOX COFACTORS DURING THE BIOSYNTHESIS OF 5,6,7,8-TETRAHYDRO-L-BIOPTERIN ? 57
12. DETERMINATION OF NEOPTERIN AND REDUCED NEOPTERINS BY RADIOIMMUNOASSAY 73
13. THE DETERMINATION OF PTERINS, BIOGENIC AMINE METABOLITES, AND AROMATIC AMINO ACIDS IN CEREBROSPINAL FLUID USING ISOCRATIC REVERSE PHASE LIQUID CHROMATOGRAPHY WITH IN SERIES DUAL CELL COULOMETRIC ELECTROCHEMICAL AND FLUORESCENCE DETECTION:- USE IN THE STUDY OF INBORN ERRORS OF DIHYDROPTERIDINE REDUCTASE AND 5,lO-METHYLENETETRAHYDROFOLATE REDUCTASE 85
14. 2. BIOCHEMISTRY AND METABOLISM OF PTERIDINES
15. TETRAHYDROBIOPTERIN METABOLISM IN THE CENTRAL NERVOUS SYSTEM 103
16. ENZYMATIC FORMATION OF H₄-BIOPTERIN IN DROSOPHILA MELANOGASTER 119
17. TETRAHYDROBIOPTERIN BIOSYNTHESIS. STUDIES ON THE REACTION OF DIHYDRONEOPTERIN TRIPHOSPHATE IN THE PRESENCE OF PHOSPHATE-ELIMINATING ENZYME FROM HUMAN LIVER 133
18. BIOSYNTHESIS OF TETRAHYDROBIOPTERIN: INCORPORATION OF LABEL FROM REDUCED PYRIDINE NUCLEOTIDE DURING FORMATION FROM DIHYDRONEOPTERIN TRIPHOSPHATE 143
19. TETRAHYDROBIOPTERIN BIOSYNTHESIS IN MAN 151
20. PHOSPHORYLATION OF UNPROTECTED D-NEOPTERIN 159
21. MEASUREMENT OF PHOSPHATE-ELIMINATING ENZYME (PEE) ACTIVITY IN LIVER BIOPSIES 169
22. INHIBITION OF THE TETRAHYDROPTERIN PATHWAY FOR BIOPTERIN COFACTOR BIOSYNTHESIS BY N-ACETYLSEROTONIN IN CELL CULTURES AND IN ENZYME PREPARATIONS AND STUDIES ON THE MECHANISM OF TETRAHYDROPTERIN FORMATION 175
23. ACTH STIMULATION OF TETRAHYDROBIOPTERIN BIOSYNTHESIS AND STEROIDOGENESIS IN ADRENAL CORTICAL TUMOR CELLS IN CULTURE 195
24. ENDOCRINE AND METABOLIC REGULATION OF GTP-CYCLOHYDROLASE ACTIVITY AND TETRAHYDROBIOPTERIN LEVELS IN RAT LIVER 209
25. TETRAHYDROBIOPTERIN SYNTHESIS AND THE ROLE OF THE COFACTOR IN THE REGULATION OF TYROSINE HYDROXYLATION IN ADRENOMEDULLARY CHROMAFFIN CELLS 221
26. BIOPTERIN METABOLISM IN HEPATOMA CELLS 237
27. HIGH BIOPTERIN CONCENTRATION IN NUCLEAR FRACTIONS OF REGIONAL RAT-BRAIN PREPARATIONS 245
28. 3. TETRAHYDROBIOPTERIN DEFICIENCIES, DIAGNOSIS AND THERAPY
29. TRANSIENT NEONATAL HYPERPHENYLALANINEMIA DUE TO DEFECTIVE SYNTHESIS OF 7,8-DIHYDROBIOPTERIN 259
30. TETRAHYDROBIOPTERIN ANALOGUES AND THEIR ABILITY TO ENTER THE RAT STRIATUM AFTER PERIPHERAL ADMINISTRATION 269
31. 4. PTERIDINES IN IMMUNOLOGY
32. IMMUNE RESPONSE- ASSOCIATED NEOPTERIN RELEASE: AN OVERVIEW 279
33. NEOPTERIN ESTIMATION an in vivo test measuring the cell mediated immune status 287
34. NEOPTERIN IN SERUM OF MAMMALS? 313
35. The Relations of HTLV-III antibodies to Neopterin and Beta-2- Microglobulin in the Serum of Patients with AIDS or Persons at Risk 319
36. INCREASE IN SERUM NEOPTERIN LEVELS IN PATIENTS RECEIVING RECOMBINANT INTE RLE UK IN-2 AND RECOMBINANT INTERFERON CC A 335
37. CLINICAL RESOLVEMENT OF MALARIA INFECTION PARALLELS DECREASE OF HIGHLY ELEVATED SERUM NEOPTERIN LEVEL 341
38. ACCUMULATION AND RELEASE OF PTERIDINES DURING STIMULATION OF HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS: PARTICIPATION IN THE CONTROL OF ANTIGEN INDUCED T-CELL EXPANSION 347
39. INTRACELLULAR CHANGES IN PTERIN RELEASING PERIPHERAL BLOOD MONONUCLEAR CELLS AFTER STIMULATION WITH INTERFERON-GAMMA 363
40. METABOLISM OF PTERINS IN HUMAN PERIPHERAL BLOOD MONONUCLEAR 369
41. URINARY NEOPTERIN EXCRETION IN THE FOLLOW UP OF BONE MARROW TRANSPLANT RECIPIENTS 379
42. PTERIDINES IN BONE MARROW TRANSPLANTATION: THE PROBLEM OF PHARMACOLOGICAL. INTERACTIONS 387
43. SERUM NEOPTERIN DETERMINATION IN BONE MARROW TRANSPLANTATION IN CHILDHOOD 403
44. RADIOIMMUNOLOGICAL DETERMINATION OF SERUM NEOPTERIN IN PATIENTS FOLLOWING BONE MARROW TRANSPLANTATION - FIRST RESULTS 411
45. PTERIDINES IN URINE AND SERUM IN KIDNEY ALLOGRAFT RECIPIENTS 419
46. RELATION OF NEOPTERIN TO INTERFERON GAMMA SERUM LEVELS IN PATIENTS WITH KIDNEY TRANSPLANTS 429
47. SERUM NEOPTERIN IN PATIENTS WITH KIDNEY ALLOGRAFTS: CLINICAL IMPLICATIONS 441
48. URINARY NEOPTERIN IN ACUTE AND CHRONIC LIVER DISEASES 453
49. URINARY NEOPTERIN EXCRETION AS AN ACTIVITY PARAMETER IN CROHN'S DISEASE 461
50. INTERFERON-GAMMA-INDUCED IN VITRO-EXCRETION OF NEOPTERIN AND 3-HYDROXYANTHRANILIC ACID BY HUMAN MACROPHAGES 473
51. 5. PTERIDINES IN CANCER AND OTHER DISEASES
52. INTERPRETATION OF THE VARIABLE NEOPTERIN EXCRETION IN PATIENTS WITH DIFFERENT TUMOR TYPES - A HYPOTHESIS 487
53. URINARY NEOPTERIN IN CHILDREN WITH MALIGNANT NEOPLASM 501
54. THE CLINICAL RELEVANCE OF THE NEOPTERIN-DETERMINATION IN PATIENTS WITH CERVICAL AND OVARIAN CANCER 515
55. DETERMINATION OF SERUM NEOPTERIN IN PATIENTS WITH ACUTE LEUKEMIA AND MALIGNANT LYMPHOMAS 525
56. NEOPTERIN - A MARKER OF THE CELLULAR IMMUNE RESPONSE IN PATIENTS WITH LUNG CANCER 535
57. Neopterin levels in different human tumors under treatment with antitumoral peptides (Factor AF2), course, response rate, prognosis 545
58. PTERIDINES IN PLASMA AND IN BLOOD CELLS FROM LEUKAEMIA PATIENTS 559
59. PTERIDINES FROM BONE MARROW AND BODY FLUIDS FROM HEALTHY DONORS AND FROM DIFFERENT PATIENTS 577
60. PTERIDINE LEVELS IN PATIENTS WITH AIDS (ACQUIRED IMMUNODEFICIENCY SYNDROME) AND WITH KAPOSI'S SARCOMA 593
61. Comparative Studies on Urinary Neopterin Excretion and other Laboratory Parameters 607
62. 6. MISCELLANEOUS
63. EFFECT OF PTERIDINES ON HYDROXY-INDOLE-O-METHYLTRANSFERASE HIOMT) 621
64. PTERIDINE VALUES IN CEREBROSPINAL FLUID IN SICK CHILDREN 635
65. EXOGENOUS FACTORS INFLUENCING THE EXCRCETION OF NEOPTERIN 643
66. NEONATAL MEGALOBLASTIC ANEMIA CAUSED BY AN INHERITED DEFECT IN THE METHIONINE SYNTHETASE REACTION IN VIVO 653
67. AUTHOR INDEX 661
68. SUBJECT INDEX 663
69. Backmatter 687