TWO-PHOTON REACTIONS OF DIHYDROFLAVIN MONONUCLEOTIDE IN GLYCOLATE OXIDASE, STUDIED BY NANOSECOND LASER PHOTOLYSIS

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. CONTENTS IX
4. Introductory Lecture
5. FLAVINS AND FLAVOPROTEINS - PAST, PRESENT, AND FUTURE 3
6. Flavin Chemistry and Physico-Chemical Studies of Flavoproteins
7. SPECTRAL PROPERTIES OF CYANOALLOXAZINES 19
8. PHOTOTAUTOMERISM OF ALLOXAZINES IN THE PRESENCE OF ACETIC ACID IN DIFFERENT MEDIA 23
9. IONISATION PROPERTIES OF REDUCED, 1,5-DIHYDROFLAVIN, RATES OF N(5)-H EXCHANGE WITH SOLVENT 27
10. PHOTOPRODUCT OF 8-HYDROXYFLAVIN 33
11. THE BREAKDOWN OF C(4A)-FLAVIN-PEROXIDES INTO FLAVIN AND A HYDROPEROXIDE IN DIFFERENT ENVIRONMENTS 37
12. FLUORESCENCE SPECTRA OF CRYSTALLINE ALLOXAZINE AND ITS METHYL DERIVATIVES 41
13. COMPARATIVE FLUORESCENCE STUDY OF PHOTOTAUTOMERISM OF LUMICHROME IN DODECYLAMMONIUM PROPIONATE REVERSED MICELLES 45
14. PICOSECOND FLUORESCENCE DYNAMICS OF REDUCED FLAVINS 49
15. REACTION MECHANISM OF FLAVIN-PHOTOSENSITIZED OXIDATION AND REDUCTION OF c-TYPE CYTOCHROMES 55
16. A SIMPLE METHOD FOR THE DETERMINATION OF REDOX POTENTIALS 59
17. ELECTRON SPIN RESONANCE SPECTRAL STUDIES OF 15N-2H FLAVIN COENZYME SEMIQUINONES IN FLAVOENZYMES 67
18. INTERFLAVIN ENERGY TRANSFER AS A TOOL TO DETERMINE GEOMETRICAL PARAMETERS IN FLAVOPROTEINS 73
19. Biosynthesis of Flavins and Flavoproteins
20. CLONING AND MOLECULAR CHARACTERIZATION OF THE RIBOFLAVIN SYNTHASE-ENCODING GENE OF Saccharomyces cerevisiae 81
21. FAD SYNTHETASE FROM BREVIBACTERIUM AMMONIAGENES : PROGRESS REPORT ON CLONING AND EXPRESSION IN ESCHERICHIA COLI 85
22. RAT BRAIN FLAVOKINASE: PURIFICATION, PROPERTIES, AND COMPARISON TO THE ENZYME FROM LIVER AND SMALL INTESTINE 89
23. FLAVIN-DEPENDENT EXPRESSION AND MODIFICATION OF 6-HYDROXY-D-NICOTINE OXIDASE 93
24. COVALENT FLAVINYLATION OF APO-6-HYDROXY-D-NICOTINE OXIDASE 101
25. Oxidases
26. THE STRUCTURE OF GLYCOLATE OXIDASE 107
27. TWO-PHOTON REACTIONS OF DIHYDROFLAVIN MONONUCLEOTIDE IN GLYCOLATE OXIDASE, STUDIED BY NANOSECOND LASER PHOTOLYSIS 115
28. CHARACTERIZATION OF GLYOOLATE OXIDASE AND AN ACTIVE SITE MUTANT 119
29. L-LACTATE MONOOXYGENASE, PROPOSED ACTIVE SITE STRUCTURE AND MECHANISM 123
30. LACTATE OXIDASE: MUTAGENESIS AND EXPRESSION OF THE MYCOBACTERIAL GENE 131
31. MOLECULAR BIOLOGICAL STUDIES ON STRUCTURE-FUNCTION RELATIONSHIP OF D-AMINO ACID OXIDASE 135
32. GENOMIC ORGANIZATION AND EXPRESSION OF D-AMINO ACID OXIDASE GENE 143
33. EXPRESSION OF MOUSE KIDNEY D-AMINO ACID OXIDASE IN ESCHERICHIA COLI: PURIFICATION AND CHARACTERIZATION OF THE RECOMBINANT PROTEIN 147
34. PURIFICATION OF RECOMBINANT MUTANT PORCINE D-AMINO ACID OXIDASES 151
35. LIMITED PROTEOLYSIS STUDIES ON THE APO- , HOLO-, HOLOENZYMEBENZOATE FORMS OF PIG KIDNEY D-AMINO ACID OXIDASE 155
36. REINVESTIGATION OF THE ROLE OF LYSINE RESIDUES IN D-AMINO ACID OXIDASE 159
37. STRUCTURAL CHANGE OF A LIGAND UPON COMPLEXATION WITH D-AMINO ACID OXIDASE 163
38. D-AMINO ACID OXIDASE EXPRESSED UNDER INDUCTION CONDITIONS IS ENZYMATICALLY ACTIVE IN MICROPEROXISOMES OF Rhodotorula gracilis 167
39. ON THE RECOMBINATION PROCESS OF APO-D-AMINO ACID OXIDASE FROM Rhodotorula gracilis 171
40. FAD ANALOGUES AS ACTIVE SITE PROBES OF Rhodotorula gracilis D-AMINO ACID OXIDASE 175
41. STRUCTURAL STUDIES ON BEEF KIDNEY D-ASPARTATE OXIDASE 179
42. MODIFICATION OF SUBSTRATE SPECIFICITY OF D-ASPARTATE OXIDASE CHEMICALLY MODIFIED BY PHENYLGLYOXAL 189
43. KINETIC ISOTOPE EFFECTS ON FLAVOPROTEIN OXIDASES 193
44. STRUCTURAL AND KINETIC ANALYSIS OF FLAVINE ADENINE DINUCLEOTIDE MODIFICATION IN ALCOHOL OXIDASE FROM METHYLOTROPHIC YEASTS 197
45. STUDIES ON YEAST-DERIVED AND MUTANT GLUCOSE OXIDASES 201
46. EFFECT OF DEGLYCOSYLATION ON THE ACTIVITY AND STABILITY OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER 205
47. ₁₅N- AND ₁₃C-NMR INVESTIGATIONS OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER 209
48. EFFECT OF HYDROGEN PEROXIDE ON BAKER'S YEAST PYRIDOXAMINEPHOSPHATE OXIDASE UNDER AEROBIC AND ANAEROBIC CONDITIONS 213
49. Monooxygenases
50. CHEMICAL FUNCTIONS OF AMINO ACID RESIDUES IN THE ACTIVE SITE OF PARAHYDROXYBENZOATE HYDROXYLASE AS STUDIED BY SITE DIRECTED MUTAGENESIS AND BIOPHYSICAL TECHNIQUES 219
51. SITE-DIRECTED MUTAGENESIS OF PARA-HYDROXYBENZOATE HYDROXYLASE FROM PSEUDOMONAS FLUORESCENS 231
52. PARTIAL SEQUENCES OF PHENOL HYDROXYLASE. AND SIMILARITIES WITH OTHER FLAVOENZYMES 235
53. 2-AMINOBENZOYL-COA MONOOXYGENASE/REDUCTASE, A NOVEL TYPE OF FLAVOPROTEIN HYDROXYLASE 239
54. STRUCTURAL STUDIES ON THE PORCINE LIVER MULTISUBSTRATE FLAVIN-CONTAINING MONOOXYGENASE 243
55. THE COFACTOR DEPENDENT INTERACTION OF MOLECULAR OXYGEN WITH PHENYLALANINE HYDROXYLASE 247
56. Flavin Dependent Bioluminescence
57. MECHANISMS OF BACTERIAL LUCIFERASE AND AROMATIC HYDROXYLASES 253
58. RANDOM AND SITE DIRECTED MUTAGENESIS OF BACTERIAL LUCIFERASE 261
59. Xenorhabdus luminescens LUCIFERASE: CLONING, SEQUENCING, AND OVEREXPRESSION OF THE ENCODING GENES AND SUBSTRATE INHIBITION OF THE ENZYME 265
60. ON THE MECHANISM OF BACTERIAL LUCIFERASE.4a,5-DIHYDROFLAVINS AS MODEL COMPOUNDS FOR REACTION INTERMEDIATES 269
61. KINETIC AND MECHANISTIC INVESTIGATION OF THE BACTERIAL LUCIFERASE REACTION 273
62. ELECTRON (CHARGE) TRANSFERS IN FLAVIN-MEDIATED LUMINESCENCE 277
63. ON THE MECHANISM OF DITHIONITE/H₂0₂-INDUCED BACTERIAL BIOLUMINESCENCE 281
64. STRUCTURE OF FP₃₉₀ INCLUDING ITS PROSTHETIC GROUP (Q-FLAVIN): PHYSIOLOGICAL SIGNIFICANCE OF LIGHT EMITTING REACTION IN LUMINOUS BACTERIA 285
65. Dehydrogenases and Electron Transferases
66. CRYSTALLOGRAPHIC STUDIES OF MEDIUM CHAIN ACYL-CoA DEHYDROGENASE FROM PIG LIVER MITOCHONDRIA 291
67. MODULATION OF FLAVIN REACTIVITY IN THE ACYL-CoA DEHYDROGENASES 299
68. DEUTERIUM SOLVENT ISOTOPE EFFECTS ON THE DISSOCIATION OF THE CHARGE TRANSFER PRODUCT COMPLEX OF MEDIUM CHAIN ACYL-CoA DEHYDROGENASE AND FPCoA 307
69. AROMATIC SUBSTRATE ANALOGUES AS MECHANISTIC PROBES FOR MEDIUM-CHAIN ACYL-COA DEHYDROGENASES 311
70. INACTIVATION OF MEDIUM-CHAIN ACYL-CoA DEHYDROGENASE FROM PIG KIDNEY BY METHYLENECYCLOPROPYL-ACETYL-CoA: IDENTIFICATION OF A NEW TYPE OF FLAVIN-INHIBITOR ADDUCT 315
71. SOME PROPERTIES OF GLU-376-GLN ACTIVE SITE MUTANT OF HUMAN MEDIUM-CHAIN ACYL-COA DEHYDROGENASE 319
72. ON THE ROLE OF GLU³⁷⁶ IN CATALYSIS OF ACYL-CoA DEHYDROGENASES 325
73. RAT LIVER MEDIUM-CHAIN ACYL CoA DEHYDROGENASES DIRECTED BY COMPLEMENTARY DNAs DIFFERING IN THEIR 5'- REGION 329
74. PURIFICATION AND SOME PROPERTIES OF FIVE DISTINCT ACYL-COA DEHYDROGENASES FROM BOVINE LIVER 333
75. THE INVOLVEMENT OF A GREEN FAD-CONTAINING ENZYME IN THE DEHYDRATION OF 5-HYDROXYVALERYL-CoA TO 4-PENTENOYL-CoA IN CLOSTRIDIUM AMINOVALERICUM 337
76. IS GLUTARYL-CoA DEHYDROGENASE FROM PARACOCCUS DBNITRIFICANS REGULATED BY SUBSTRATE AND PRODUCT BINDING? 341
77. TWO FLAVO-DEHYDROGENASES CATALYZING DESATURATION OF A-RING OF 3-KETOSTEROIDS: 3-KETOSTEROID-Δ¹-DEHYDROGENASE AND 3-KETOSTEROID- Δ⁴-DEHYDROGENASE FROM NOCARDIA CORALLINA 345
78. THE CLONING OF OLD YELLOW ENZYME 349
79. CRYSTALLIZATION AND CHARACTERIZATION OF OLD YELLOW ENZYME 353
80. STUDIES ON OLD YELLOW ENZYME RECONSTITUTED WITH THE ACTIVE SITE PROBE, 8-FLUORO-8-DEMETHYL FMN. COVALENT MODIFICATION AND 19F-NMR 357
81. TWO-DIMENSIONAL NMR STUDIES OF DESULFOVIBRIO VULGARIS AND MEGASPHAERA ELSDENII FLAVODOXIN 361
82. ¹³C-NMR STUDIES ON CYANYLATED MEGASPHAERA ELSDENII FLAVODOXIN 369
83. FLAVIN BINDING SITE GEOMETRY IN ANABAENA 7120 FLAVODOXIN. PROGRESS IN DETERMINING THE FLAVODOXIN SOLUTION STRUCTURE 377
84. ¹³C-NMR STUDY OF NADPH-ADRENODOXIN REDUCTASE RECONSTITUTED WITH ¹³C-ENRICHED FAD 381
85. COVALENT PHOSPHORUS GROUPS IN FLAVOPROTEINS: THE PHOSPHODIESTER LINKAGE IN AZOTOBACTER (OP) FLAVODOXIN 385
86. DIFFERENCES IN FLAVIN MOTIONAL DYNAMICS IN OXIDIZED AND REDUCED CLOSTRIDIAL FLAVODOXIN AS ASSESSED BY MOLECULAR DYNAMICS SIMULATIONS AND FLUORESCENCE ANISOTROPY 393
87. A WORKING PROPOSAL FOR THE ROLE OF THE APOPROTEIN IN DETERMINING THE REDOX POTENTIAL OF THE FLAVIN IN FLAVOPROTEINS: CORRELATIONS BETWEEN POTENTIALS AND FLAVIN PKS 399
88. POLARIZED ABSORPTION SPECTRA OF FLAVIN MONONUCLEOTIDE IN FLAVODOXIN CRYSTALS 405
89. STRUCTURAL ANALYSIS OF FULLY REDUCED A. NIDULANS FLAVODOXIN 409
90. THE SITE-DIRECTED MUTAGENESIS OF BACTERIAL FLAVODOXINS 415
91. STRUCTURAL CHARACTERIZATION OF SITE MUTANTS OF CLOSTRIDIAL FLAVODOXIN 423
92. REDOX PROPERTIES OF WILD-TYPE AND MUTANT FLAVODOXINS FROM DBSULFOVIBRIO VULGARIS (HILDENBOROUGH) 429
93. CHARACTERIZATION OF FLRUODOHIN FROM THE EUKARIOTE Chlorella fusca 437
94. COMMON STRUCTURAL FEATURES OF A RED ALGAL FLAVODOXIN AND CYANOBACTERIAL FERREDOXINS 441
95. CLONING OF THE FERREDOXIN-NADP⁺ -OXIDOREDUCTASE GENE AND OVEREXPRESSION OF A SYNTHETIC FLAVODOXIN GENE FROM THE CYANOBACTERIA ANABAENA PCC 7119 445
96. STRUCTURE/FUNCTION OF SPINACH FERREDOXIN:NADP⁺ OXIDOREDUCTASE 449
97. SPINACH FERREDOXIN-NADP⁺ REDUCTASE: CHARACTERIZATION OF THE ENZYMES EXPRESSED IN ESCHERICHIA COLI 457
98. PURIFICATION AND CHARACTERIZATION OF FERREDOXIN-NADP⁺ OXIDOREDUCTASE FROM NON-PHOTOSYNTHETIC TISSUES 461
99. FERREDOXIN BINDING SITE OF FERREDOXIN-NADP+ REDUCTASE AS EXPLORED BY LIMITED PROTEOLYSIS AND MUTAGENESIS 465
100. FERREDOXINS AND FERREDOXIN-NADP+ REDUCTASES FROM ANABAENA PCC 7119 AND SPINACH: ELECTROSTATIC EFFECTS ON INTRACOMPLEX ELECTRON TRANSFER 469
101. COVALENTLY STABILIZED ELECTRON-TRANSFER COMPLEXES: A REDOX ACTIVE COMPLEX BETWEEN SPINACH FERREDOXIN-NADP+ REDUCTASE AND DESULFOVIBRIO VULGARIS FLAVODOXIN 475
102. STRUCTURAL ANALYSIS OF THE REQUIREMENTS FOR THE FORMATION OF AN ELECTRON TRANSFER COMPLEX 479
103. INTRAMOLECULAR ELECTRON TRANSFER BETWEEN FERREDOXIN-NADP⁺ REDUCTASE AND FLAVODOXIN: A LASER PHOTOLYSIS STUDY 483
104. EVALUATION OF SECONDARY STRUCTURE PREDICTIONS FOR ENZYMES RELATED TO FERREDOXIN REDUCTASE 487
105. ROLES OF FOUR CYSTEINE RESIDUES AND LYSINE 110 IN HUMAN NADHCYTOCHROME b₅ REDUCTASE STUDIED BY SITE-DIRECTED MUTAGENESIS 491
106. Disulfide Reductases
107. PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES - OVERVIEW OF THE FAMILY AND SOME PROPERTIES OF THIOREDOXIN REDUCTASE ALTERED BY SITE DIRECTED MUTAGENESIS: C135S AND C138S 497
108. STRUCTURAL CHANGES ON BINDING FAD AND FAD-ANALOGUES AND ON SITE-DIRECTED MUTAGENESIS OF GLUTATHIONE REDUCTASE 505
109. EXPLORATION OF THE COENZYME AND SUBSTRATE SPECIFICITY OF GLUTATHIONE REDUCTASE AND ITS SUBUNIT ASSEMBLY 513
110. SUBUNIT INTERACTIONS IN THE GLUTATHIONE REDUCTASE FROM ESCHERICHIA COLI 521
111. ACTIVE-SITE MUTANTS OF THE GLUTATHIONE REDUCTASE FROM ESCHERICHIA COLI 525
112. YEAST GLUTATHIONE REDUCTASE: DETERMINATION OF THE REDOX POTENTIAL USING NADH AND NAD⁺ 529
113. GLUTATHIONE .REDUCTASE PHOTOREDUCTION BY ONE-ELECTRON DONORS: STABILIZATION OF FAD SEMIQUINONE SPECIES BY COMPLEXATION 533
114. MOUSE GLUTATHIONE REDUCTASE cDNA. ALIGNMENT OF THE DEDUCED AMINO ACID SEQUENCE WITH THE STRUCTURAL DOMAINS OF THE HUMAN ENZYME 537
115. FAD BINDING PROPERTIES OF BRAIN GLUTATHIONE REDUCTASE 545
116. THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF LIPOAMIDE DEHYDROGENASE FROM Azotobacter vinelandii AT 2 . 2 A RESOLUTION 549
117. SITE-DIRECTED MUTAGENESIS STUDIES ON LIPOÄMIDE DEHYDROGENASE FROM AZOTOBACTER VINELANDII 557
118. LIPOAMIDE DEHYDROGENASE FROM AZOTOBACTER VINELANDII - KINETIC STUDIES ON WILD TYPE AND MUTANT ENZYMES 565
119. BINDING STUDIES OF THE DIHYDROLIPOAMIDE DEHYDROGENASE COMPONENT (E3) IN THE PYRUVATE DEHYDROGENASE COMPLEX FROM AZOTOBACTER VI NELANDII 569
120. PROPERTIES OF LIPOAMIDE DEHYDROGENASE FROM E. COLI MODIFIED BY SITE-DIRECTED MUTAGENESIS: K53R AND I184Y 573
121. REPLACEMENT OF THE ACTIVE SITE BASE IN E. COLI LIPOAMIDE DEHYDROGENASE: SPECTRAL AND KINETIC CHARACTERIZATION 577
122. CHARACTERIZATION OF THE ACTIVE SITE MUTANTS (C44S, C49S) OF ESCHERICHIA COLI LIPOAMIDE DEHYDROGENASE 581
123. FAD-INDUCED DIMERIZATION OF APO-LIPOAMIDE DEHYDROGENASE FROM AZOTOBACTER VINELANDII AND PSEUDOMONAS FLUORESCENS 585
124. PIG HEART LIPOAMIDE DEHYDROGENASE: SOLVENT KINETIC ISOTOPE EFFECTS 589
125. Km EFFECTS ASSOCIATED WITH SITE-DIRECTED MUTATIONS THAT REDUCE THE VELOCITY OF ONE HALF REACTION OF A PING PONG MECHANISM 593
126. HUMAN DIHYDROLIPOAMIDE DEHYDROGENASE: EXPRESSION AND FUNCTIONAL ASPECTS OF NORMAL AND MODIFIED ENZYMES 599
127. STRUCTURE, FUNCTION AND EVOLUTION OF MULTIPLE LIPOAMIDE DEHYDROGENASES OF PSEUDOMONAS PUTIDA 603
128. DIHYDROLIPOAMIDE DEHYDROGENASE FROM HALOBACTERIUM VOLCANII: PURIFICATION, CHARACTERISATION AND MOLECULAR CLONING 607
129. ELECTRON-TRANSFERRING FLAVOPROTEINS FROM GLYCINEMETABOLIZING ANAEROBIC BACTERIA 611
130. THE THREE-DIMENSIONAL STRUCTURE OF MERCURIC ION REDUCTASE FROM BACILLUS SP. STRAIN RC607 615
131. ON THE MECHANISM OF MERCURIC REDUCTASE: AN ALTERNATING SITES HYPOTHESIS 627
132. THE STREPTOCOCCAL NADH PEROXIDASE AND NADH OXIDASE: STRUCTURAL AND MECHANISTIC ASPECTS 639
133. SEQUENCE FINGERPRINTS FOR THE DISULFIDE REDUCTASES: APPLICATION TO THE STREPTOCOCCAL NADH PEROXIDASE 647
134. THE STRUCTURE OF NADH PEROXIDASE FROM STREPTOCOCCUS FAECALIS 651
135. PEROXIDE MODIFICATION OF MONOALKYLATED GLUTATHIONE REDUCTASE: EVIDENCE FOB STABILIZATION OF AN ACTIVE-SITE CYSTEJUE-SULFENIC ACID INTERMEDIATE 655
136. ARTIFICIAL FLAVINS AS PROBES OF THE ACTIVE-SITE ENVIRONMENT AND REDOX BEHAVIOR OF THE STREPTOCOCCAL NADH PEROXIDASE 659
137. REACTIVITY OF THE STREPTOCOCCAL NADH OXIDASE RECONSTITUTED WITH ARTIFICIAL FLAVINS 663
138. NADH PEROXIDASE: pH PROFILES AND KINETIC ISOTOPE EFFECTS 667
139. Complex Flavoproteins
140. AN ACTIVE SITE PROBE (6-N3-FAD) STUDY OF MILK XANTHINE OXIDASE 673
141. ELECTRON TRANSFER IN XANTHINE OXIDASE CONTAINING CHEMICALLY MODIFIED FLAVINS 677
142. STUDIES OF ELECTRON TRANSFER IN XANTHINE OXIDASE 681
143. EVIDENCE FOR PARTICIPATION OF THE PHOSPHOSERYL RESIDUE OF XANTHINE OXIDASE IN THE HYDROXYLATION EVENT DURING ENZYME CATALYSIS 685
144. ENDOR STUDIES OF THE MOLYBDENUM CENTRE OF XANTHINE OXIDASE 691
145. MILK XANTHINE DEHYDROGENASE 695
146. MOLECULAR CLONING OF cDNA FOR RAT LIVER XANTHINE DEHYDROGENASE AND ITS PRIMARY STRUCTURE 699
147. CYSTEINE RESIDUES RESPONSIBLE FOR DEHYDROGENASE-OXIDASE CONVERSION OF RAT LIVER XANTHINE DEHYDROGENASE 703
148. TOWARDS AN EXPRESSION SYSTEM FOR SITE-DIRECTED MUTAGENESIS STUDIES OF XANTHINE DEHYDROGENASE (Drosophila melanogaster rosy GENE) 707
149. FUMARATE REDUCTASE FROM ESCHERICHIA COLI: MOLECULAR APPROACHES TO THE UNDERSTANDING OF THE FUNCTION OF ITS PROSTHETIC GROUPS 711
150. SITE DIRECTED MUTAGENESIS OF THE PUTATIVE LIGANDS OF THE [3Fe-4S] CLUSTER OF FUMARATE REDUCTASE OF ESCHERICHIA COLI 719
151. SITE-DIRECTED MUTAGENESIS OF THE ACTIVE SITE OF Escherichia coli FUMARATE REDUCTASE 723
152. CLONING OF THE FLAVO-SUBUNIT OF HUMAN SUCCINATE DEHYDROGENASE 727
153. THE FLAVOPROTEIN FRACTION OF NADH-UBIQUINONE REDUCTASE FROM BOVINE MITOCHONDRIA: RELATIONSHIP TO A BACTERIAL NAD-REDUCING HYDROGENASE 731
154. TWO RELATED FORMS OF THE RESPIRATORY CHAIN NADH DEHYDROGENASE (COMPLEX I) IN NEOROSPORA MITOCHONDRIA 735
155. INTRAMOLECULAR ELECTRON TRANSFER IN TRIMETHYLAMINE DEHYDROGENASE FROM BACTERIUM W₃A₁ 739
156. STRUCTURE DETERMINATION OF AN IRON-SULFUR FLAVOPROTEIN 743
157. GLUTAMATE SYNTHASE FROM AZOSPIRILLUM BRASILENSE: ROLE OF FLAVINS AND IRON SULFUR CENTERS 749
158. KINETIC AND STRUCTURAL STUDIES ON GLUTAMATE SYNTHASE FROM AZOSPIRILLUM BRASILENSE 755
159. SEQUENCE MOTIFS IN THE FLAVIN DOMAIN OF NADH:NITRATE REDUCTASE 761
160. THERMODYNAMICS AND ELECTRON TRANSFER IN NITRATE REDUCTASE 765
161. REDOX INTERCONVERSION OF NITRATE REDUCTASE ACTIVITY CATALYZED BY PHOTOEXCITED FLAVINS 769
162. ON SOME ASPECTS OF THE CATALYSIS OF LACTATE DEHYDROGENATION BY FLAVOCYTOCHROME b₂ 773
163. SUBSTITUTION OF TYR143 BY PHE IN FLAVOCYTOCHROME b₂ AFFECTS ELECTRON TRANSFER FROM FLAVIN TO ACCEPTORS 783
164. ISOLATION AND CHARACTERIZATION OF THE FLAVIN DOMAIN OF FLAVOCYTOCHROME b₂ EXPRESSED INDEPENDENTLY IN E. cot 787
165. DIRECT MEASUREMENT OF INTRAMOLECULAR ELECTRON TRANSFER BETWEEN THE FMN AND HEME COFACTORS OF YEAST FLAVOCYTOCHROMES b₂ BY FLASH PHOTOLYSIS: EVIDENCE FOR PRODUCT-MEDIATED CONFORMATIONAL GATING 791
166. CLONING AND SEQUENCING OF THE HANSENULA ANOMALA FLAVOCYTOCHROME b₂ GENE 797
167. HANSENULA ANOMALA FLAVOCYTOCHROME b₂ : LOSS OF ELECTRON DONOR REACTIVITY OF THE FLAVO SEMI QUI NONE BY PYRUVATE BINDING 801
168. CRYSTAL STRUCTURE OF P-CRESOL METHYLHYDROXYLASE AT 3.0 A RESOLUTION 805
169. INTERACTIONS IN MULTI-SUBUNIT REDOX PROTEINS: NMR STUDY OF THE CYTOCHROME SUBUNIT OF p-CRESOLMETHYLHYDROXYLASE 809
170. Folate Dependent Flavoproteins
171. STEREOCHEMISTRY OF REDUCTION OF METHYLENETETRAHYDROFOLATE TO METHYLTETRAHYDROFOLATE CATALYZED BY MAMMALIAN METHYLENETETRAHYDROFOLATE REDUCTASE 815
172. RECONSTITUTION OF APOPHOTOLYASE WITH PTERIN AND/OR FLAVIN DERIVATIVES 819
173. CHEMICAL MODIFICATION OF CORYNEBACTERIUM SARCOSINE OXIDASE HITH IODOACETAMIDE 827
174. Flavoproteins of Medical Relevance
175. MOLECULAR STUDIES OF TRYPANOTHIONE REDUCTASE: AN ANTIPARASITIC TARGET ENZYME 833
176. LIPOAMIDE DEHYDROGENASE AND TRYPANOTHIONE REDUCTASE FROM TRYPANOSOMA CRUZI. THE CAUSATIVE AGENT OF CHAGAS'DISEASE 843
177. BINDING OF FAD TO BCNU-TREATED APOGLUTATHIONE REDUCTASE 847
178. THE EGRAC AS A MEASURE OF THE RIBOFLAVIN STATUS IN MAN. TITRATION OF HEMOLYS ATE FAD WITH APOGLUTATHIONE REDUCTASE 851
179. STUDIES ON THE ROLE OF SERINE-127 IN NADH-CYTOCHROME b₅ REDUCTASE BY SITE-DIRECTED MUTAGENESIS 855
180. ADRENODOXIN REDUCTASE OF MITOCHONDRIAL CYTOCHROME P450 SYSTEMS: STRUCTURE AND REGULATION OF EXPRESSION 859
181. SQUALENE EPOXIDASE: THE ELUSIVE FLAVOENZYME OF STEROL BIOSYNTHESIS 865
182. EXPRESSION OF MEMBRANE-BOUND FLAVOENZYMES IN YEAST 869
183. BINDING OF MPP⁺ AND ITS ANALOGS TO THE ROTENONE/PIERICIDIN SITE OF NADH DEHYDROGENASE (NADH-UBIQUINONE OXIDOREDUCTASE) 873
184. MEMBRANE-BOUND AND CYTOSOLIC 45 kDa PROTEINS IN HUMAN NEUTROPHILS AND HL-60 CELLS 877
185. COVALENTLY-BOUND FLAVIN IN PEROXISOMAL L-PIPECOLIC ACID OXIDASE FROM PRIMATES 881
186. DIFFERENCES IN PROPERTIES OF XANTHINE DEHYDROGENASE AND XANTHINE OXIDASE 885
187. BIOCHEMICAL CHARACTERIZATION OF A MUTANT HUMAN MEDIUM-CHAIN ACYL-COA DEHYDROGENASE PRESENT IN PATIENTS HAVING DEFICIENT ACTIVITY 895
188. RESTRICTION ANALYSIS OF THE HUMAN MEDIUM-CHAIN ACYL COA DEHYDROGENASE (ACADM) GENOMIC REGION 901
189. Non Redox-Active Flavoproteins
190. ACETOLACTATE SYNTHASE: A DEVIANT FLAVOPROTEIN 907
191. List of Participants 915
192. Author Index 921
193. Subject Index 927
194. Backmatter 947