"ACTIVATION CHROMATOGRAPHY" - A NEW APPROACH FOR DEALING WITH RENATURATION PROBLEMS IN PROTEIN ENGINEERING STUDIES

Chapters in this book

1. Frontmatter I
2. Preface V
3. Contents VII
4. NON-RADIOACTIVE PLAQUE AND COLONY SCREENING WITH DIGOXIGENIN- 11-dUTP TAILED OLIGONUCLEOTIDES 1
5. OLIGONUCLEOTIDE-DIRECTED MUTATION CONSTRUCTION 19
6. DNA DIAGNOSTICS - APPLICATIONS AND NEW ANALYTICAL TECHNIQUES 37
7. FREE FLOW ELECTROPHORESIS FOR THE SEPARATION AND PURIFICATION OF BIOPOLYMERS 69
8. SEPARATION OF PROTEINS AND NUCLEIC ACIDS USING "TENTACLE-TYPE" ION EXCHANGERS 99
9. IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY OF RECOMBINANT PROTEINS 117
10. CAPILLARY ELECTROPHORESIS AND ION SPRAY MASS SPECTROMETRY, NEW POWERFUL METHODS FOR SEPARATION AND CHARACTERIZATION OF PROTEINS AND NUCLEOTIDES 133
11. RENATURATION OF RECOMBINANT, DISULFIDE-BONDED PROTEINS FROH "INCLUSION BODIES" 149
12. "ACTIVATION CHROMATOGRAPHY" - A NEW APPROACH FOR DEALING WITH RENATURATION PROBLEMS IN PROTEIN ENGINEERING STUDIES 173
13. PROGRESS IN THE DEVELOPMENT OF A C—TERMINAL SEQUENCING METHOD FOR PROTEINS AND PEPTIDES 187
14. DETERMINATION OF THE ENANTIOMERIC COMPOSITION OF AMINO ACIDS BY ENANTIOSELECTIVE GAS CHROMATOGRAPHY 213
15. PREDICTION OF PEPTIDE EPITOPES IN PROTEINS 231
16. DEVELOPMENT OF SPECIFIC IMMUNOASSAYS FOR THYMOSINS 251
17. BIOAFFINITY SPIN LABELING OF BIOMEMBRANE PROTEINS 271
18. CROSS-LINKING BETWEEN NUCLEIC ACIDS AND PROTEINS 297
19. PHOTOAFFINITY LABELLING AND ITS COMBINATION WITH BIOTIN/AVIDIN COMPLEXING IN THE ANALYSIS OF PEPTIDE HORMONE RECEPTORS 305
20. HOW DO YOU GET LARGE PROTEIN CRYSTALS? TWO FAMILIAR LABORATORY METHODS IMPROVED AND SIMPLIFIED 343
21. CIRCULAR DICHROISM AS A TOOL FOR INVESTIGATING MACROMOLECULAR STRUCTURE 367
22. MODELLING OF PROTEIN STRUCTURES ON THE BASIS OF SEQUENCE DATA 395
23. ANALYSIS OF PROTEIN STRUCTURE IN SOLUTION BY TWO-DIMENSIONAL NMR SPECTROSCOPY: 2D-¹H NMR INVESTIGATION OF RIBONUCLEASE T AND ITS COMPLEXES WITH 2 ' - and 3'-GUANOSINE MONOPHOSPHATES 423
24. SUBJECT INDEX 439