1.3 Vectors for Gene Transfer and Expression in Animal Cells

Chapters in this book

1. I-IV I
2. Preface V
3. Contents VII
4. Contributors List XVII
5. 1. Genetic Manipulation of Mammalian Cells
6. 1.1 Heterologous Expression of Genes in Mammalian Cells 3
7. 1.2 Permanent Gene Expression in Mammalian Cells: Gene Transfer And Selection 33
8. 1.3 Vectors for Gene Transfer and Expression in Animal Cells 65
9. 1.4 Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells 87
10. 1.5 Isolation of Recombinant Cell Clones Exhibiting High-Level Expression of the Introduced Gene 121
11. 1.6 Genetic Engineering of Antibodies and Derivatives from Mammalian Cells 139
12. 1.7 Safety Evaluation of Products Derived From Mammalian Cell Lines 171
13. 2. Biological Aspects of Animal Cells
14. 2.1 Metabolic Control of Animal Cell Culture Processes 193
15. 2.2 Glycosylation: A Post-Translational Modification 233
16. 3. Cell Cultivation Technology
17. 3.1 Bioreactors Designed for Animal Cells 279
18. 3.2 Hydrodynamic Properties in Bioreactors 319
19. 3.3 Kinetics and Simulation of Animal Cell Processes 345
20. 3.4 On-line and Off-line Process Analysis 373
21. 4. Down-Stream Processing
22. 4.1 Principles of Product Extraction from Cell Culture and Purification for Pharmaceutical Proteins 413
23. 4.2 Validation of Downstream Processes 453
24. Index 483
25. 492 492