Chapter 2 Metabolic engineering of microbes
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Birthe Halmschlag
Abstract
Microbes have developed a multitude of possibilities to utilize CO2 as carbon source. While the CO2 fixation pathways are optimized through evolution, the production of valuable products thereof is rarely a natural trait. To efficiently use autotrophic microbes in industrial biotechnology, metabolic engineering can contribute to further yield and to rate improvement of CO2 fixation as well as to the exploitation of microbial synthesis capacities. The optimized metabolic engineering workflow includes consecutive design-built-test cycles. The computational design of metabolic networks built on genome sequencing data can direct strain engineering and thereby reduce experimental work. With growing numbers of available gene editing methods and expanding molecular toolkits, the construction of genetically engineered organisms greatly accelerated. The development of high-throughput screening systems for clone identification and phenotyping is a prerequisite to keep pace with the progress in cell factory design. The herein described metabolic engineering techniques will greatly expand the potential of autotrophic microbes in industrial biotechnology processes.
Abstract
Microbes have developed a multitude of possibilities to utilize CO2 as carbon source. While the CO2 fixation pathways are optimized through evolution, the production of valuable products thereof is rarely a natural trait. To efficiently use autotrophic microbes in industrial biotechnology, metabolic engineering can contribute to further yield and to rate improvement of CO2 fixation as well as to the exploitation of microbial synthesis capacities. The optimized metabolic engineering workflow includes consecutive design-built-test cycles. The computational design of metabolic networks built on genome sequencing data can direct strain engineering and thereby reduce experimental work. With growing numbers of available gene editing methods and expanding molecular toolkits, the construction of genetically engineered organisms greatly accelerated. The development of high-throughput screening systems for clone identification and phenotyping is a prerequisite to keep pace with the progress in cell factory design. The herein described metabolic engineering techniques will greatly expand the potential of autotrophic microbes in industrial biotechnology processes.
Chapters in this book
- Frontmatter I
- Contents V
- List of authors VII
- Chapter 1 A short recapitulation of the autotrophic metabolism 1
- Chapter 2 Metabolic engineering of microbes 19
- Chapter 3 Protein engineering 47
- Chapter 4 Gas fermentation 85
- Chapter 5 Introduction to autotrophic cultivation of microalgae in photobioreactors 113
- Chapter 6 Synthetic biology of cyanobacteria 131
- Chapter 7 Algal biotechnology 173
- Chapter 8 Biocatalytic applications of autotrophic organisms 207
- Chapter 9 Photocatalysis to promote cell-free biocatalytic reactions 247
- Chapter 10 Electroautotrophs: feeding microbes with current for CO2 fixation 277
- Chapter 11 Cupriavidus necator – a broadly applicable aerobic hydrogen-oxidizing bacterium 297
- Chapter 12 Poly(3-hydroxybutyrate) as renewable resource 319
- Chapter 13 Applications of mixed microbial cultures in industrial biotechnology 353
- Chapter 14 Economic framework of autotrophic processes 385
- Index 397
Chapters in this book
- Frontmatter I
- Contents V
- List of authors VII
- Chapter 1 A short recapitulation of the autotrophic metabolism 1
- Chapter 2 Metabolic engineering of microbes 19
- Chapter 3 Protein engineering 47
- Chapter 4 Gas fermentation 85
- Chapter 5 Introduction to autotrophic cultivation of microalgae in photobioreactors 113
- Chapter 6 Synthetic biology of cyanobacteria 131
- Chapter 7 Algal biotechnology 173
- Chapter 8 Biocatalytic applications of autotrophic organisms 207
- Chapter 9 Photocatalysis to promote cell-free biocatalytic reactions 247
- Chapter 10 Electroautotrophs: feeding microbes with current for CO2 fixation 277
- Chapter 11 Cupriavidus necator – a broadly applicable aerobic hydrogen-oxidizing bacterium 297
- Chapter 12 Poly(3-hydroxybutyrate) as renewable resource 319
- Chapter 13 Applications of mixed microbial cultures in industrial biotechnology 353
- Chapter 14 Economic framework of autotrophic processes 385
- Index 397
