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Carotenoid Production by Phaffia rhodozyma Using Raw Glycerol as an Additional Carbon Source
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Carolina M. Silva
Veröffentlicht/Copyright:
23. Oktober 2012
Abstract
This paper presents pure glycerol and raw glycerol as additional carbon sources in the production of carotenoids by Phaffia rhodozyma NRRL Y-17268. Batch cultures in shaken flasks were performed in order to verify the effects of temperature cultivation (20 and 25ºC) and pure glycerol concentration (0, 10 and 40 g.L-1). The increase from 20 to 25ºC led to a significant increase (p<0.05) in cell concentration for all the pure glycerol concentrations, reaching the maximum levels of 8.9 g.L-1 of biomass (168 h), specific production of carotenoids (SPC) of 91.0 μg.g-1 (72 h) and volumetric production of carotenoids (VPC) of 2.0 μg.mL-1 (168 h) with 40 g.L-1 of pure glycerol at 25ºC. The use of 40 g.L-1 of raw glycerol at the same temperature resulted in a carotenogenic production showing no significant difference (p>0.05) in pure glycerol, reaching the maximum values of 8.3 g.L-1 of biomass (168 h) and within 120 h SPC 275.5 μg.g-1 and VPC 1.7 μg.mL-1. Therefore, the pure glycerol and raw glycerol can be used as additional carbon sources in the culture medium of P. rhodozyma.
Published Online: 2012-10-23
©2012 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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- Optimization of Microwave Assisted Extraction Conditions for Total Flavonoids in Toona Sinensis Leaves Using Response Surface Methodology
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