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Micelle Based Spectrofluorimetric Determination of Chloroquine Phosphate in Commercial Formulation and Human Plasma

  • M. Rasul Jan , Jasmin Shah , M. Aamir Javed and Nageen Yousaf
Published/Copyright: November 17, 2014
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Tenside Surfactants Detergents
From the journal Tenside Surfactants Detergents Volume 51 Issue 6

Abstract

Simple, sensitive and economic spectrofluorimetric method has been described for the determination of chloroquine phosphate in pure form, pharmaceutical formulations and spiked human plasma. In the developed method, fluorescence intensity of chloroquine phosphate was enhanced with 0.4 M sodium dodecyl sulfate in basic media of pH 10 at room temperature. The excitation and emission wavelengths of the fluorescent chloroquine phosphate are 330 nm and 369 nm respectively. The developed approach has a broad linear range (0.03–5 μg mL−1) with a correlation coefficient of 0.9976. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 3.38 × 10−3 μg mL−1 and 1.12 × 10−2 μg mL−1 respectively. The common additives and co-administered medications were investigated for their interferences effect in the assay. The method was validated statistically through recovery studies and successfully applied to chloroquine phosphate determination in bulk powder, pharmaceutical preparation and spiked human plasma samples. The percent recoveries were found to be in the range of 99.42–100.46 % for bulk powder, 97.48–101.21 % for pharmaceutical formulations and 96.49–98.48 % for spiked human plasma.

Kurzfassung

Es wird eine einfache, empfindliche und wirtschaftliche spektrofluorimetrische Methode für die Bestimmung von Chloroquindiphosphat in reiner Form, in pharmazeutischen Formulierungen und im menschlichen Plasma beschrieben. Mit der entwickelten Methode konnte die Intensität der Fluoreszenz von Chloroquindiphosphat durch Zugabe von 0,4 M Natriumdodecylsulfat im basischen Medium (pH 10) bei Raumtemperatur gesteigert werden. Die Anregungs- und Emissionswellenlängen von Chloroquindiphosphat sind 330 nm und 369 nm. Die entwickelte Methode hat einen breiten linearen Bereich (0.03–5 μg mL−1) mit einem Korrelationskoeffizienten von 0,9976. Die Nachweisgrenze (limit of detection LOD) und die Bestimmungsgrenze (limit of quantification LOQ) betrugen 3.38 × 10−3 μg mL−1 bzw. 1.12 × 10−2 μg mL−1. Die herkömmlichen Additive und Kombinationsmedikamente wurden im Hinblick auf Interferenzen in der Probe untersucht. Die Methode wurde mittels Studien zur Wiederfindung von Chloroquindiphosphat in reinem Pulver, in pharmazeutischen Zubereitungen und in aufgestockten Humanplasmaproben statistisch validiert. Die prozentualen Wiederfindungsraten lagen zwischen 99.42–100.46 % beim reinen Pulver, zwischen 97.48–101.21 % bei den pharmazeutischen Zubereitungen und zwischen 96.49–98.48 % bei den aufgestockten Humanplasmaproben.

Key words: Spectrofluorimetry; micelle; sodium dodecyl sulfate (SDS); chloroquine phosphate; spiked human plasma sample
Stichwörter: Spektrofluorimetrie; Mizelle; Natriumdodecylsulfat (SDS); Chloroquindiphosphat; aufgestockten Humanplasmaprobe
* Correspondence address, Mrs. Prof. J. Shah, Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan. Fax: 92-91-9216652, E-Mail:

Professor Jasmin Shah, PhD: Professor of Analytical Chemistry in the Institute of Chemical Sciences, University of Peshawar. Actively involved in method development for quantification of drugs and different types of organic and inorganic species.

Professor Muhammad Rasul Jan: Professor of Analytical and Environmental Chemistry. Actively involved in method development for pharmaceutical drugs, pesticides and solid phase extraction of organic compounds.

Mr. Aamir Javed: Ph. D. scholar working under the supervion of Professor Rasul Jan and Professor Jasmin Shah.

Ms. Nageen Yousaf: Ph. D. scholar working under the supervion of Professor Jasmin Shah and Professor Rasul Jan.

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Received: 2013-12-18
Accepted: 2014-07-11
Published Online: 2014-11-17
Published in Print: 2014-11-17

© 2014, Carl Hanser Publisher, Munich

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  2. Contents
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  4. Rapid Assessment of Glass Etching/Corrosion using the Quartz Crystal Microbalance with Dissipation Monitoring, QCM-D
  5. Micelle Based Spectrofluorimetric Determination of Chloroquine Phosphate in Commercial Formulation and Human Plasma
  6. Novel Surfactants
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  8. Synthesis and Properties of Guerbet Hexadecyl Sulfate
  9. Synthesis and Characterization of N-alkyl-N′-glucosylhexanediamine Surfactant
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  15. Percolative Behavior Models Based on Artificial Neural Networks for Electrical Percolation of AOT Microemulsions in the Presence of Crown Ethers as Additives
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https://doi.org/10.3139/113.110333
Keywords for this article
Spectrofluorimetry; micelle; sodium dodecyl sulfate (SDS); chloroquine phosphate; spiked human plasma sample

Articles in the same Issue

  1. Contents/Inhalt
  2. Contents
  3. Application
  4. Rapid Assessment of Glass Etching/Corrosion using the Quartz Crystal Microbalance with Dissipation Monitoring, QCM-D
  5. Micelle Based Spectrofluorimetric Determination of Chloroquine Phosphate in Commercial Formulation and Human Plasma
  6. Novel Surfactants
  7. Synthesis of Rice Bran Fatty Acids (RBFAs) Based Cationic Surfactants and Evaluation of Their Performance Properties in Combination with Nonionic Surfactant
  8. Synthesis and Properties of Guerbet Hexadecyl Sulfate
  9. Synthesis and Characterization of N-alkyl-N′-glucosylhexanediamine Surfactant
  10. Mizellar Catalysis
  11. Methanesulfonic Acid as a More Efficient Catalyst for the Synthesis of Lauraldehyde Glycerol Acetal
  12. Surfactant Assisted Enhancement of Bioremediation Rate for Hexavalent Chromium by Water Extract of Siris (Albizia lebbeck) Sawdust
  13. Physical Chemistry
  14. Synergistic Interactions in Mixed W/O Microemulsions of Cationic Gemini and Anionic Surfactants
  15. Percolative Behavior Models Based on Artificial Neural Networks for Electrical Percolation of AOT Microemulsions in the Presence of Crown Ethers as Additives
  16. Junction Characteristics System Based on Composite Organic Semiconductors: Polystyrene/Polyaniline Doped by [BMIM] [BF4] Ionic Liquid
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