Optimal glucose and inoculum concentrations for production of bioactive molecules by Paenibacillus polymyxa RNC-D
-
Nadja Serrano
,
Ligia Rodrigues
Abstract
The production of antimicrobial metabolites by Paenibacillus polymyxa RNC-D was assessed. Two process variables, glucose and inoculum concentrations, were evaluated at different levels (5–40 g L−1, and at φ r = 2.5–5.0 %, respectively), and their effects on biomass formation, minimal inhibitory concentration (MIC) against Escherichia coli, and surface tension reduction (STR) were studied. When the fermentation process was carried out under non-optimised conditions, the biomass, MIC, and STR achieved the following values: 0.6 g L−1, 1 g L−1, and 18.4 mN m−1, respectively. The optimum glucose (16 g L−1) and inoculum volume ratio (φ r = 5.0 %) were defined in order to maximise the biomass formation, with a low value of MIC and high STR of extract. The experiments carried out under optimal conditions showed the following values for the dependent variables: biomass concentration 2.05 g L−1, MIC 31.2 μg mL−1, and STR 10.7 mN m−1, which represented improvement of 241.7 %, 96.9 %, and 41.9 % for the responses of biomass, MIC, and STR, respectively. This is the first recorded study on the optimisation of culture conditions for the production of antimicrobial metabolites of P. polymyxa RNC-D, and constitutes an important step in the development of strategies to modulate the production of antimicrobial molecules by this microorganism at elevated levels.
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© 2012 Institute of Chemistry, Slovak Academy of Sciences
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Articles in the same Issue
- Quantitative analysis of two adulterants in Cynanchum stauntonii by near-infrared spectroscopy combined with multi-variate calibrations
- Study of deoxynivalenol effect on metallothionein and glutathione levels, antioxidant capacity, and glutathione-S-transferase and liver enzymes activity in rats
- Biodegradation of tobacco waste by composting: Genetic identification of nicotine-degrading bacteria and kinetic analysis of transformations in leachate
- Optimal glucose and inoculum concentrations for production of bioactive molecules by Paenibacillus polymyxa RNC-D
- Electrodialysis of oxalic acid: batch process modeling
- Relationship between the decrease of degree of polymerisation of cellulose and the loss of groundwood pulp paper mechanical properties during accelerated ageing
- Improved hydrothermal synthesis of MoS2 sheathed carbon nanotubes
- Fabrication of a micro-direct methanol fuel cell using microfluidics
- Determination of pK a of benzoic acid- and p-aminobenzoic acid-modified platinum surfaces by electrochemical and contact angle measurements
- Theoretical enthalpies of formation and structural characterisation of halogenated nitromethanes and isomeric halomethyl nitrites
- Assessment of role of rosmarinic acid in preventing oxidative process of low density lipoproteins
