Determination of Kinetic and Stoichiometric Parameters of Pseudomonas putida F1 by Chemostat and In Situ Pulse Respirometry
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Catarina S Oliveira
The applicability of pulse respirometry, for the estimation of kinetic and stoichiometric parameters in pure cultures was evaluated by comparison with traditional chemostat method. Pseudomonas putida F1 was cultured in a continuous stirred tank reactor, using glucose as sole carbon source. The reactor was operated under steady-state with six dilution rates, ranging from 0.06 to 0.35 h-1. Substrate and biomass concentration were measured and used to estimate kinetic and stoichiometric parameters, according to the Monod model. An in situ respirometry method was also applied to the reactor, with the injection of pulses of glucose from 19 to 97 mg L-1. The respirograms obtained were used to estimate the kinetic and stoichiometric parameters according to ASM1 and ASM3 models. No significance difference was observed between parameters estimated by chemostat and respirometric methods. The glucose affinity constant was from 0.4 to 0.7 mg L-1, the maximum specific growth rate was from varying from 0.14 to 0.20 h-1, and the growth yield was from 0.41 to 0.67. These results confirm that in situ pulse respirometry is a suitable method for kinetic and stoichiometric parameters estimation.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Articles in the same Issue
- Article
- Process Modeling & Control: A Special Issue of Chemical Product & Process Modeling
- Determination of Kinetic and Stoichiometric Parameters of Pseudomonas putida F1 by Chemostat and In Situ Pulse Respirometry
- Exponential Observer Design for State Estimation in a Class of Anaerobic Sulfate Reducing Bioreactor
- From Reacting to Predicting Technologies: A Novel Performance Monitoring Technique Based on Detailed Dynamic Models
- Nonlinear Analysis of Heterogeneous Model for an Industrial Ammonia Reactor
- Iterative Design of Dynamic Experiments in Modeling for Optimization of Innovative Bioprocesses
- Dynamic Process and Accident Simulations as Tools to Prevent Industrial Accidents
- Modeling Disturbance Dynamics to Improve Controller Performance in Industrial Loops
- On the Design of an In-Line Control System for a Vial Freeze-Drying Process: The Role of Chamber Pressure
- A Takagi-Sugeno Fuzzy Dynamic Model of a Concentric-Tubes Heat Exchanger
- Design of Dynamics of a Recuperative Catalytic Combustor: Enhancement in Operation and Control
- Formulation and Spectral Reduction of the Dynamical Model of a Circulating Fluidized Bed Combustor
