Rational engineering of specialized metabolites in bacteria and fungi
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Ramsay Soup Teoua Kamdem
,
Omonike Ogbole
Abstract
Bacteria and fungi have a high potential to produce compounds that display large structural change and diversity, thus displaying an extensive range of biological activities. Secondary metabolism or specialized metabolism is a term for pathways and small molecule products of metabolism that are not mandatory for the subsistence of the organism but improve and control their phenotype. Their interesting biological activities have occasioned their application in the fields of agriculture, food, and pharmaceuticals. Metabolic engineering is a powerful approach to improve access to these treasured molecules or to rationally engineer new ones. A thorough overview of engineering methods in secondary metabolism is presented, both in heterologous and epigenetic modification. Engineering methods to modify the structure of some secondary metabolite classes in their host are also intensively assessed.
Acknowledgements
RSTK and PS acknowledge financial support from the Alexander von Humboldt (AvH) through return fellowship. FNK acknowledges a return fellowship and an equipment subsidy from the Alexander von Humboldt Foundation, Germany. Financial support for this work is acknowledged from a ChemJets fellowship from the Ministry of Education, Youth and Sports of the Czech Republic awarded to FNK.
Glossary of Terms used in Cheminformatics of Natural Products
Dereplication: A strategy used for the previous identification of known compounds in a complex sample, based on comparing spectroscopic and spectrometric data with the previous reported
Molecular Networking: computational tool used to assist dereplication procedures, in which is possible to visualize similarities in compound fragmentation (LC-MS/MS)
Heterologous expression: refers to the expression of a gene or part of a gene in a host organism, which does not naturally have this gene or gene fragment. Insertion of the gene in the heterologous host is performed by recombinant DNA technology.
Combinatorial Biosynthesis: Application of genetic engineering to modify biosynthetic pathways to natural products in order to produce new and altered structures using nature’s biosynthetic machinery
OSMAC: One Strain Many Compounds.
Phylogenetics: is the study of evolutionary relationships among biological entities - often species, individuals or genes.
NRPS: Non-ribosomal polyketides synthetase
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Colored pigments
- Rational engineering of specialized metabolites in bacteria and fungi
- Color fundamentals
- Introduction to pulp and paper industry: Global scenario
- Dissolving pulp production: Cellulases and xylanases for the enhancement of cellulose accessibility and reactivity
- Iron blue pigments
Articles in the same Issue
- Frontmatter
- Colored pigments
- Rational engineering of specialized metabolites in bacteria and fungi
- Color fundamentals
- Introduction to pulp and paper industry: Global scenario
- Dissolving pulp production: Cellulases and xylanases for the enhancement of cellulose accessibility and reactivity
- Iron blue pigments
