Newtonoate as an active principle of Newtonia griffoniana for anxiolytic activity in Swiss mice
-
Sefirin Djiogue
,
Mc Jesus Kinyok
Abstract
Background: Newtonia griffoniana (Mimosaceae) is a Central African rain forest tree, whose bark extracts are used in Cameroonian folk medicine for the treatment of anxiety and sleep disorders.
Methods: We evaluated the anxiolytic effects of N. griffoniana stem bark methanol extract and its major isolated constituent 2,3,4-trihydroxybutylpentatriacontanoate (newtonoate) on the elevated plus maze.
Results: Significant increases in the percentage of entries into open arms were induced by both N. griffoniana extract (100 and 150 mg/kg BW; p<0.01) and newtonoate (doses of 3 and 15 mg/kg BW; p<0.05). Conversely, decreases in the percentage of entries into closed arms were observed at the same doses. In addition, N. griffoniana methanol extract (100 mg/kg) and the isolated newtonoate (30 mg/kg) induced significant (p<0.01 and p<0.05, respectively) increases in the time spent in the open arms, while inducing a decrease in the time spent in the closed arms. Newtonoate treatment also decreased head dipping number at doses of 3 and 15 mg/kg, while N. griffoniana methanol extract induced the same effect at 200 mg/kg.
Conclusions: These results suggest that N. griffoniana bark extract has anxiolytic properties, which justify its use in folk medicine. Such effects are at least partly mediated by newtonoate.
Acknowledgments
The authors are thankful to Dr Gaetan Bayiha for MS and NMR analysis, and to the members of the Laboratory of Animal Physiology of the University of Yaoundé 1 for their technical assistance.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Articles in the same Issue
- Frontmatter
- Review
- Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis
- Preclinical Studies
- Immunomodulatory activity of methanol leaf extracts of Cameroonian medicinal plants
- Dietary supplementation with green tea extract promotes enhanced human leukocyte activity
- Newtonoate as an active principle of Newtonia griffoniana for anxiolytic activity in Swiss mice
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Articles in the same Issue
- Frontmatter
- Review
- Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis
- Preclinical Studies
- Immunomodulatory activity of methanol leaf extracts of Cameroonian medicinal plants
- Dietary supplementation with green tea extract promotes enhanced human leukocyte activity
- Newtonoate as an active principle of Newtonia griffoniana for anxiolytic activity in Swiss mice
- Antibacterial activity of combination of synthetic and biopolymer non-woven structures
- Amelioration of chronic inflammation and oxidative stress indices in diabetic Wistar rats using methanol leaf extract of Bridelia micrantha (Hochst) Baill. (Euphorbiaceae)
- Possible anti-diarrhoeal potential of ethanol leaf extract of Chromolaena odorata in castor oil-induced rats
- Inhibition of AKT signaling by supercritical CO2 extract of mango ginger (Curcuma amada Roxb.) in human glioblastoma cells
- Clinical Studies
- A cross-sectional study on knowledge and attitude toward Traditional Chinese Medicine (TCM) among adults in selected regions of Malaysia
- Assessment of the impacts of traditional Persian medical schemes and recommendations on functional chronic constipation compared to a classic medicine lactulose, a randomized clinical trial
