Pioglitazone attenuate level of myeloperoxidases and nitic oxide in psoriatic lesion: a proof-of-concept study in a imiquimod induced psoriasis model in rat
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Oishani Chatterjee
Abstract
Objectives
Psoriasis is a persistent autoimmune inflammatory condition that is primarily affecting the skin. Pioglitazone (PGZ), a peroxisome proliferator activated receptor gamma (PPARγ) agonist, has been reported to have anti-inflammatory effects. However, the role of PGZ in psoriatic disease remains unclear. In this study, we aimed to repurpose the use of the PGZ for the treatment of psoriasis.
Methods
To investigate its efficacy, we employed an imiquimod (IMQ)-induced rat model. Wistar rats are randomly allocated to four different groups. Group, I served as a negative control, Group II IMQ control, Group III was treated with pioglitazone hydrogel and Group IV received standard drug betamethasone cream. PASI score was monitored on every alternative day and on day 7 animals were sacrificed and histopathology of skin was performed. Level of nitric oxide (NO) and myeloperoxidase (MPO) was also performed using established methods.
Results
The results of the experiment revealed that treatment with PGZ significantly (p<0.05) reduced redness, scaling, and skin thickening, surpassing the effectiveness of standard drugs. Our result also indicates that PGZ significantly (p<0.05) inhibits the release of both MPO and NO from the psoriatic lesions.
Conclusions
PGZ effectively reduces the severity of psoriasis possibly by inhibiting the accumulation of neutrophil at the psoriatic area which indirectly regulates the release of NO in the affected area. Our study showed we can repurpose the PGZ for the management of psoriasis.
Acknowledgments
The Director and Principal of Guru Nanak Institute of Pharmaceutical Science and Technology, Kolkata, India, and the management of JIS Group, Kolkata India are highly acknowledged for encouragement and support.
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Research ethics: All the experimental procedures and protocols were reviewed and approved by the Institutional Animal Ethical Committee (CCSEA approval no. GNIPST/IAEC/s/2023/DS-02).
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Informed consent: Not applicable.
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Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors state no conflict of interest.
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Research funding: None declared.
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Data availability: Enquiries about data availability should be directed to the authors.
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Articles in the same Issue
- Frontmatter
- Editorials
- Can small molecule GLP-1 agonists be the next first-line drugs in type-2 diabetes mellitus?
- From Dr. Google to CE-marked medical devices: need for ethical and legal safeguards
- Minireview
- Antioxidant therapy for hepatic diseases: a double-edged sword
- Reviews
- Effect of cannabinoids in mild-to-moderate cases of Crohn’s disease as compared to placebo: a systematic review and meta-analysis of randomised controlled trials
- Evidences and therapeutic advantages of donanemab in the treatment of early Alzheimer’s disease
- Alzheimer’s disease and clinical trials
- Original Articles
- Pioglitazone attenuate level of myeloperoxidases and nitic oxide in psoriatic lesion: a proof-of-concept study in a imiquimod induced psoriasis model in rat
- Protecting cardiomyocytes from hypoxia-reoxygenation injury, empaglifozin and liraglutide alone or in combination?
- Gasotransmitters do not prevent changes in transepithelial ion transport induced by hypoxia followed by reoxygenation
- High-intensity combination exercise has the highest effect on increasing serum irisin and interleukin 6 levels in women with obesity
- Impact of heat stress on thermal balance, hydration and cortical response among outdoor workers in hot environment – an exploratory report from North East India
- Maternal separation influences hepatic drug-metabolizing CYP450 gene expression without pathological changes in adult mice
- Case Report
- Preoperative CT diagnosis of perforated Meckel’s diverticulitis in a young patient: a case report
Articles in the same Issue
- Frontmatter
- Editorials
- Can small molecule GLP-1 agonists be the next first-line drugs in type-2 diabetes mellitus?
- From Dr. Google to CE-marked medical devices: need for ethical and legal safeguards
- Minireview
- Antioxidant therapy for hepatic diseases: a double-edged sword
- Reviews
- Effect of cannabinoids in mild-to-moderate cases of Crohn’s disease as compared to placebo: a systematic review and meta-analysis of randomised controlled trials
- Evidences and therapeutic advantages of donanemab in the treatment of early Alzheimer’s disease
- Alzheimer’s disease and clinical trials
- Original Articles
- Pioglitazone attenuate level of myeloperoxidases and nitic oxide in psoriatic lesion: a proof-of-concept study in a imiquimod induced psoriasis model in rat
- Protecting cardiomyocytes from hypoxia-reoxygenation injury, empaglifozin and liraglutide alone or in combination?
- Gasotransmitters do not prevent changes in transepithelial ion transport induced by hypoxia followed by reoxygenation
- High-intensity combination exercise has the highest effect on increasing serum irisin and interleukin 6 levels in women with obesity
- Impact of heat stress on thermal balance, hydration and cortical response among outdoor workers in hot environment – an exploratory report from North East India
- Maternal separation influences hepatic drug-metabolizing CYP450 gene expression without pathological changes in adult mice
- Case Report
- Preoperative CT diagnosis of perforated Meckel’s diverticulitis in a young patient: a case report
