Raw Glycerol as Substrate for the Production of Yeast Biomass
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Elisane Odriosolla Santos
,
Mariano Michelon
Abstract: The possibility of using raw glycerol as a substrate for yeast biomass production as a source of proteins was investigated. Biomass concentration, protein content and total protein content of four yeasts (Yarrowia lipolytica NRRL YB-423, Candida lipolytica NRRL Y-1095, Candida utilis NRRL Y-900 and Candida rugosa NRRL Y-95) were determinate, comparing analytical grade glycerol and raw glycerol. Y. lipolytica NRRL YB-423 has been selected as promising for cultivation in a raw glycerol-based medium, mainly due to the higher biomass concentration in relation to the other strains. For this strain, four different culture media were tested. The best results were obtained with 50 g/L glycerol, 5.5 g/L ammonium phosphate, 5.5 potassium dihydrogen phosphate, 1 g/L ammonium sulphate, 0.25 g/L magnesium sulphate, 0.021 g/L calcium chloride dehydrate, 1 g/L yeast extract, 1 g/L peptone, pH adjusted to 5.5. In these conditions, it was possible to obtain 17.8 ± 0.6 g/L maximum biomass concentration, 18.2 ± 1.0% protein content and 3.1 ± 0.1 g/L total protein production. These results represent a 1.2-fold increase in biomass concentration, a 1.5-fold increase in protein content and a 1.9-fold increase in total protein production in relation to the results obtained with the previously medium composition.
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©2013 by Walter de Gruyter Berlin / Boston
Articles in the same Issue
- Masthead
- Masthead
- Drying Kinetics, Biochemical and Functional Properties of Products in Convective Drying ofAnchovy (Engraulis anchoita) Fillets
- A Rheological Model for Cupuassu (Theobroma grandiflorum) Pulp at Different Concentrations and Temperatures
- Pear Drying: Thermodynamics Studies and Coefficients of Convective Heat and Mass Transfer
- Evaluation of Thin-Layer Drying Models and Artificial Neural Networks for Describing Drying Kinetics of Canola Seed in a Heat Pump Assisted Fluidized Bed Dryer
- Relating Rice Grain Quality to Conditions during Sun Drying
- Modeling of Basil Leaves Drying by GA–ANN
- Effect of Pulsed Vacuum Treatment on Mass Transfer and Mechanical Properties during Osmotic Dehydration of Pineapple Slices
- Raw Glycerol as Substrate for the Production of Yeast Biomass
- Effect of Aminoethoxyvinylglycine and Methyl Jasmonate on Individual Phenolics and Post-harvest Fruit Quality of Three Different Japanese Plums (Prunussalicina Lindell)
- Process Optimization for Foam Mat-Tray Drying of Passiflora edulis Flavicarpa Pulp and Characterization of the Dried Powder
- Evaluation of the Freezing and Thawing Cryoconcentration Process on Bioactive Compounds Present in Banana Juice from Three Different Cultivars
- Effects of Defatted Flaxseed Addition on Rheological Properties of Wheat Flour Slurry
- Disease Identification and Grading of Pomegranate Leaves Using Image Processing and Fuzzy Logic
- Calculation of the Effective Diffusion Coefficients in Drying of Chemical and Mechanical Pretreated Rosehip Fruits (Rosa eglanteria L.) with Selected Mass Transfer Models
- Assessment of the Physico-mechanical, Chemical and Colour Characteristics of Potatoes Depending on Tuber Size and Cultivar
- Moisture Sorption Characteristics of Dakuwa (Nigerian Cereal/Groundnut Snack)
- A New Alternative Real-Time Method to Monitoring Dough Behavior during Processing Using Wireless Sensor Technology
Articles in the same Issue
- Masthead
- Masthead
- Drying Kinetics, Biochemical and Functional Properties of Products in Convective Drying ofAnchovy (Engraulis anchoita) Fillets
- A Rheological Model for Cupuassu (Theobroma grandiflorum) Pulp at Different Concentrations and Temperatures
- Pear Drying: Thermodynamics Studies and Coefficients of Convective Heat and Mass Transfer
- Evaluation of Thin-Layer Drying Models and Artificial Neural Networks for Describing Drying Kinetics of Canola Seed in a Heat Pump Assisted Fluidized Bed Dryer
- Relating Rice Grain Quality to Conditions during Sun Drying
- Modeling of Basil Leaves Drying by GA–ANN
- Effect of Pulsed Vacuum Treatment on Mass Transfer and Mechanical Properties during Osmotic Dehydration of Pineapple Slices
- Raw Glycerol as Substrate for the Production of Yeast Biomass
- Effect of Aminoethoxyvinylglycine and Methyl Jasmonate on Individual Phenolics and Post-harvest Fruit Quality of Three Different Japanese Plums (Prunussalicina Lindell)
- Process Optimization for Foam Mat-Tray Drying of Passiflora edulis Flavicarpa Pulp and Characterization of the Dried Powder
- Evaluation of the Freezing and Thawing Cryoconcentration Process on Bioactive Compounds Present in Banana Juice from Three Different Cultivars
- Effects of Defatted Flaxseed Addition on Rheological Properties of Wheat Flour Slurry
- Disease Identification and Grading of Pomegranate Leaves Using Image Processing and Fuzzy Logic
- Calculation of the Effective Diffusion Coefficients in Drying of Chemical and Mechanical Pretreated Rosehip Fruits (Rosa eglanteria L.) with Selected Mass Transfer Models
- Assessment of the Physico-mechanical, Chemical and Colour Characteristics of Potatoes Depending on Tuber Size and Cultivar
- Moisture Sorption Characteristics of Dakuwa (Nigerian Cereal/Groundnut Snack)
- A New Alternative Real-Time Method to Monitoring Dough Behavior during Processing Using Wireless Sensor Technology
