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Rab1 interacts directly with the β2-adrenergic receptor to regulate receptor anterograde trafficking
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Maha M. Hammad
Published/Copyright:
June 1, 2012
Abstract
Very little is understood about the trafficking of G protein-coupled receptors (GPCRs) from the endoplasmic reticulum (ER) to the plasma membrane. Rab guanosine triphosphatases (GTPases) are known to participate in the trafficking of various GPCRs via a direct interaction during the endocytic pathway, but whether this occurs in the anterograde pathway is unknown. We evaluated the potential interaction of Rab1, a GTPase known to regulate β2-adrenergic receptor (β2AR) trafficking, and its effect on export from the ER. Our results show that GTP-bound Rab1 interacts with the F(x)6LL motif of β2AR. Receptors lacking the interaction motif fail to traffic properly, suggesting that a direct interaction with Rab1 is required for β2AR anterograde trafficking.
Received: 2011-12-12
Accepted: 2012-1-23
Published Online: 2012-06-01
Published in Print: 2012-06-01
©2012 by Walter de Gruyter Berlin Boston
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Keywords for this article
β2-adrenergic receptor;
ER export;
G protein-coupled receptor;
Rab GTPase;
trafficking
Articles in the same Issue
- Review
- The critical role of adrenomedullin and its binding protein, AMBP-1, in neuroprotection
- Minireview
- Ubiquilins in the crosstalk among proteolytic pathways
- Protein Structure and Function
- A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A
- The composite nature of the interaction between nuclear receptors EcR and DHR38
- Tmem27 dimerization, deglycosylation, plasma membrane depletion, and the extracellular Phe-Phe motif are negative regulators of cleavage by Bace2
- New ketomethylene inhibitor analogues: synthesis and assessment of structural determinants for N-domain selective inhibition of angiotensin-converting enzyme
- Cadherin-related protein 24 induces morphological changes and partial cell polarization by facilitating direct cell-cell interactions
- Molecular Medicine
- Analysis of tissue and salivary nicotinamide N-methyltransferase in oral squamous cell carcinoma: basis for the development of a noninvasive diagnostic test for early-stage disease
- Cell Biology and Signaling
- Mitochondrial protein import pathways are functionally conserved among eukaryotes despite compositional diversity of the import machineries
- Small molecule inhibitors of the c-Jun N-terminal kinase (JNK) possess antiviral activity against highly pathogenic avian and human pandemic influenza A viruses
- Matriptase-2 (TMPRSS6) is directly up-regulated by hypoxia inducible factor-1: identification of a hypoxia-responsive element in the TMPRSS6 promoter region
- Rab1 interacts directly with the β2-adrenergic receptor to regulate receptor anterograde trafficking
- Masthead
- Masthead
