Potential therapeutic properties of broccoli extract and soy isoflavones on improvement endometriosis and involved oxidative parameters

Soudabeh Sabetian; Parimah Archin Dialameh; Nader Tanideh; Behrooz Gharesifard; Moslem Ahmadi; Maryam Valibeigi; Perikala Vijayananda Kumar; Sarah Siahbani; Bahia Namavar Jahromi

doi:10.1515/hmbci-2023-0071

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Potential therapeutic properties of broccoli extract and soy isoflavones on improvement endometriosis and involved oxidative parameters

Soudabeh Sabetian , Parimah Archin Dialameh , Nader Tanideh , Behrooz Gharesifard , Moslem Ahmadi , Maryam Valibeigi , Perikala Vijayananda Kumar , Sarah Siahbani and Bahia Namavar Jahromi

Published/Copyright: July 23, 2024

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 45 Issue 3

Abstract

Objectives

In Endometriosis is a gynecological disorder characterized by the growth of endometrial tissue outside the uterine cavity that is associated with chronic pelvic pain and subfertility. The purpose of the study was to investigate the effect of broccoli extract (BE) alone and in combination with soy isoflavones (SI) on endometrial implants in female rat.

Methods

In this study, endometriosis was induced surgically in 40 mature female rats. The rats were divided into 5 groups that were treated by oral gavage for 6 weeks with 0.5 mL of saline 0.9 %/day (control group), BE (3,000 mg/kg/day), SI (50 mg/kg/day), BE/soy isoflavones (BE 3000 mg/kg/day + soy isoflavones 50 mg/kg/day) and diphereline as a standard medication (3 mg/kg) intramuscularly. At the end of treatments, the volume and histopathology of the endometrial implants were compared among the 5 groups. The serum levels of oxidative parameters including superoxide dismutase (SOD), malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were also compared between the groups. The volume of the implants significantly decreased in diphereline group (p=0.002).

Results

The histopathological grade of endometrial implants in BE/SI and diphereline group were significantly decreased compared to the control group (p=0.001). The serum levels of SOD in BE group were enhanced significantly in comparison to the control group (p=0.034).

Conclusions

BE in combination with SI decreased the growth and histopathologic grades of transplanted endometrial implants. These herbal compounds may have the potential therapeutic effect to be used as an alternative medication for the treatment of endometriosis.

Lay summary

Endometriosis happens when tissue similar to the uterus tissue grows outside of the uterus. It can be associated with severe pelvic pain and make several difficulties to get pregnant. Since the use of hormonal drugs is prohibited when planning to get pregnant and due to the common recurrence of lesions after surgery, scientists have been encouraged to study regarding the efficacies of natural products on improvement of endometriosis. In this study, it is represented that the dietary consumption of broccoli extract combined with soy isoflavones may have potential beneficial properties on clinical symptoms of endometriosis.

Keywords: endometriosis; herbal compounds; broccoli extract; soy isoflavones

Corresponding author: Bahia Namavar Jahromi, Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Hazrat-e-Zeynab Hospital, Shiraz University of Medical Sciences, Shiraz, Iran, E-mail: namavarprc@gmail.com

Funding source: Vice Chancellor for Research of Shiraz University of Medical Sciences

Award Identifier / Grant number: 97-01-50-19167

Acknowledgments

We would like to thank the Vice-Chancellor for Research of Shiraz University of Medical Sciences for their financial support with grant number 97-01-50-19167.

Research ethics: The Medical Ethics Committee of Shiraz University of Medical Sciences approved this study with the reference number IR.SUMS.REC.1398.176.
Informed consent: Not applicable.
Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors state no conflict of interest.
Research funding: This article has been financially supported by the Vice Chancellor for Research of Shiraz University of 266 Medical Sciences with the grant number of 97-01-50-19167.
Data availability: The raw data can be obtained on request from the corresponding author.

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Received: 2024-01-08

Accepted: 2024-07-02

Published Online: 2024-07-23

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2023-0071

Keywords for this article

endometriosis; herbal compounds; broccoli extract; soy isoflavones