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Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower

  • A. Solodenko EMAIL logo
Published/Copyright: July 5, 2018
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Abstract

Simple sequence repeats (SSR) polymorphism of 34 microsatellite loci (LG1, 8 and 13) was studied in lines carrying the downy mildew resistance genes Pl and lines with no Pl. The microsatellite loci ORS328 and ORS781 were selected as markers for genes Pl6 and Pl8 in lines HA 335 and QHP-1, respectively. Markers were identified for gene PlARG in RHA 419 and some accessions of H. argophyllus. The SSR markers ORS509, ORS605, ORS610, ORS1182 and ORS1039 were proven to reliably identify the parental line carrying PlARG gene, control and select the heterozygous F1 hybrids and identify homozygous genotypes in F2 generations. Obtained results indicate the necessity of validation of the markers in various germplasm pools and breeding collections. The SSR markers that are tightly linked to Pl6, Pl8, PlARG would be useful in the sunflower breeding. PlARG homozygous F2 segregants, developed and identified with marker assisted selection in this study, are recommended for further breeding as a new source of genetically determined resistance to downy mildew.

References

Abratti, G., Bazzalo, M.E., Grondona, M., 2004. Mapping a rust resistance gene and the downy mildew Pl8 gene in sunflower. In: Proceedings of 16th International Sunflower Conference, Fargo, North Dacota, USA, pp. 615–622.Search in Google Scholar

Ajaala, F., 1984. Population and Evolutionary Genetics: A Primer, Mir, Moscow, pp. 232. (in Russian).Search in Google Scholar

Davar, R., Darvishzaden, R., Majd, A., Ghosta, Y., Sarrafi, A., 2010. QTL mapping of partitial resistance to basal stem rot in sunflower using recombinant inbred lines. Phytopatol Mediterranean 49: 330–341.Search in Google Scholar

Doyle, J.J., Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Search in Google Scholar

Duble, C., Hahn, V., Knapp, S., Bauer, E., 2004. PlARG from H. argophyllus is unlinked to other known downy mildew resistance genes in sunflower. Theoretical and Applied Genetics 109: 1083–1086.10.1007/s00122-004-1722-9Search in Google Scholar PubMed

Gegil, M., Slabaugh, M., Berry, S., 2001. Candidate disease resistance genes in sunflower cloned using conserved NBS motifs: Genetic mapping and linkage to the downy mildew resistance gene Pl1. Genome 44(2): 205–212.10.1139/g00-110Search in Google Scholar PubMed

Gulya, T.J., 2007. Distribution of Plasmopara halstedii races from sunflower around the world. In: Lebeda, A., Spencer-Phillips, P.T.N. (eds.) Advances in Downy Mildew Research Proceedings of 2nd International Downy Mildew Symposium, Olomouc, Czech. Republic, Palacky University and JOLA, Kastelec na Hane, Czech Republic, pp. 121–134.Search in Google Scholar

Gupta, P.K., Varshney, R.K., 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113: 163–185.10.1023/A:1003910819967Search in Google Scholar

Heesacker, A., Kishore, V.K., Gao, W., Tang, S., Kolkman, J.M., Gingle, A., Matvienko, M., Kozik, A., Michelmore, R., Lai, Z., 2008. SSRs and INDELs mined from the sunflower EST database: Abundance, polymorphisms and cross-taxa utility. Theoretical and Applied Genetics 117: 1021–1029.10.1007/s00122-008-0841-0Search in Google Scholar PubMed

Jan, C.C., Fernandez-Martinez, J.M., Ruso, J., Munoz-Ruz, J., 2002. Registration of four sunflower germplasms with resistance to Orobanche cumana race F. Crop Sciences 42: 2217–2218.10.2135/cropsci2002.2217Search in Google Scholar

Jan, C.C., Tan, A.S., Gulya, T.J., 2004. Registration of four downy mildew resistant sunflower germplasms. Crop Science 44: 1887.10.2135/cropsci2004.1887Search in Google Scholar

Jocić, S., Cvejić, S., Hladni, N., Miladinović, D., Miklič, V., 2010. Development of sunflower genotypes resistant to downy mildew. Helia 33(53): 173–180.10.2298/HEL1053173JSearch in Google Scholar

Miller, J., Gulya, T., Seiler, G., 2002. Registration of five fertility restorer sunflower germplasms. Crop Science 42: 989–991.10.2135/cropsci2002.0989Search in Google Scholar

Miller, J.F., Gulya, T.J., 1991. Inheritance of resistance to race 4 of downy mildew derived from interspecific crosses in sunflower. Crop Science 31: 40–43.10.2135/cropsci1991.0011183X003100010009xSearch in Google Scholar

Miller, J.F., Seiler, G.J., 2003. Registration of five oilseed maintainer (HA 429-Ha 433) sunflower germplasm lines. Crop Science 43: 2313–2314.10.2135/cropsci2003.2313Search in Google Scholar

Mulpuri, S., Liu, Z., Femg, J., 2009. Inheritance and molecular mapping of a downy mildew resistance gene, Pl13 in cultivated sunflower (Helianthus annuus L.). Theoretical and Applied Genetics 119: 795–803.10.1007/s00122-009-1089-zSearch in Google Scholar PubMed

Paniego, N., Eschaide, M., Munoz, M., Fernandez, L., Torales, S., Faccoi, P., Fuxan, I., Carrera, M., Zandomeny, R., Suarez, E., Hopp, H., 2002. Microsatellite isolation and characterization in sunflower (Helianthus annuus L.). Genome 45: 34–43.10.1139/g01-120Search in Google Scholar PubMed

Panković, D., Radovanović, N., Jocić, S., Šatović, Z., Škorić, D., 2007. Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730. Plant Breeding 126(4): 440–444.10.1111/j.1439-0523.2007.01376.xSearch in Google Scholar

Qi, L.L., Long, Y.M., Ma, G.J., Markell, S.G., 2015. Map saturation and SNP marker development for the rust resistance genes (R4, R5, R13a, and R13b) in sunflower (Helianthus annuus L.). Molecular Breeding 35: 196–220.10.1007/s11032-015-0380-8Search in Google Scholar

Radwan, O., Bouzidi, M., Vear, F., Philippon, J., Tourvieille De Labrouche, D., Nicolas, P., Mouzeyar, S., 2003. Identification of non-TIR-NBS-LRR markers linked to Pl5/Pl8 locus for resistance to the downy mildew in sunflower. Theoretical and Applied Genetics 106: 1438–1446.10.1007/s00122-003-1196-1Search in Google Scholar PubMed

Seiler, G., Christie, B., Choo, T., 1991. Registration of 13 downy mildew tolerant interspecific sunflower germplasm lines derived from wild annual species. Crop Science 31: 1714–1716.10.2135/cropsci1991.0011183X003100060093xSearch in Google Scholar

Solodenko, A.Y., Varenyuk, B.F., Alexandrova, O.Y., Sivolap, Y.M., 2013. Race composition of downy mildew and resistance of sunflower lines. PBGI Scientific Papers Digest 22(62): 117–123. (In Ukrainian).Search in Google Scholar

Talia, P., Nishinakamasu, V., Hopp, H.E., Heinz, R.A., Paniego, N., 2010. Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in previously developed sunflower map. Electronic Journal Biotechnology 13: 1–14.10.2225/vol13-issue6-fulltext-14Search in Google Scholar

Tang, S., Heesacker, A., Kishore, V.K., Fernandez, A., Sadik, E.S., Cole, G., Knapp, S.J., 2003. Genetic mapping of the Or 5 gene for resistance to Orobanche race E in sunflower. Crop Science 43: 1021–1028.10.2135/cropsci2003.1021Search in Google Scholar

Tang, S., Yu, J.K., Slabaugh, M.B., Shintani, D.K., Knapp, S.J., 2002. Simple sequences repeat map of the sunflower genome. Theoretical and Applied Genetics 105: 1124–1136.10.1007/s00122-002-0989-ySearch in Google Scholar PubMed

Wieckhorst, S., Bachlava, E., Duble, C., Tang, S., Gao, W., Saski, C., Knapp, S., Schön, C.-C., Hahn, V., Bauer, E., 2010. Fine mapping of the sunflower resistance locus PlARG introduced from the wild species Helianthus argophyllus. Theoretical and Applied Genetics 121: 1633–1644.10.1007/s00122-010-1416-4Search in Google Scholar PubMed PubMed Central

Yu, J., Tang, S., Slaubaugh, M.B., Heesacker, A., Cole, G., Herring, M., 2003. Towards a saturated molecular genetic linkage map for cultivated sunflower. Crop Science 43: 367–387.10.2135/cropsci2003.3670Search in Google Scholar

Received: 2017-11-03
Accepted: 2018-04-02
Published Online: 2018-07-05
Published in Print: 2018-07-26

© 2018 Walter de Gruyter GmbH, Berlin/Boston

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