Home Adhesion and proliferation of cells and bacteria on microchip with different surfaces microstructures
Article
Licensed
Unlicensed Requires Authentication

Adhesion and proliferation of cells and bacteria on microchip with different surfaces microstructures

  • Yi Zhou , Yu Xiao , Yulei Qiu , Huipin Yuan , Clemens A. van Blitterswijk , Xuedong Zhou , Xiaoming Xu and Chongyun Bao EMAIL logo
Published/Copyright: December 18, 2015

Abstract

Surface microstructure of implant materials is an essential factor for soft tissue healing around the implant. The purpose of this study was to explore the effect of different microchip surface microstructures on the adhesion and proliferation of cells and bacteria. Hydroxyapatite (HA) microchips with different microstructures (linear, decussate, circular and triangular) and their polydimethylsiloxane (PDMS) replica chips were prepared. Myoblast cells (C2C12), Staphylococcus aureus and Porphyromonas gingivalis were seeded on these chips to investigate the effect of different surface microstructures on the adhesion and proliferation. The results indicated that different surface microstructure in the same size did not show much difference on adhesion and proliferation of cell and bacteria; compared to microstructure region (grain ca. 2 μm), the cells preferred to adhesion and proliferate in the blank area (grain ca. 260 nm), in contrast, the bacteria were significantly preferable to the microstructure regions. In conclusion, it might be better for the implant materials to be manufactured in submicron-scale rather than micro-scale to improve the proliferation of cells and to inhibit the adhesion and growth of bacteria.


Corresponding author: Chongyun Bao, PhD, DDS, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China, Fax: +86 28 85501233, E-mail:

Acknowledgments

The authors thank Dr. Max Groenendijk, Lightmotif, Twente University, 7500 AE Enschede, The Netherlands, for his help in fabricating the samples of micro-patterns. This work was supported by Natural Science Foundation of China (81371181, 81171005), Ministry of Education, Fund for the Doctoral(20100181110058).

References

[1] Arima Y, Iwata H. Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials 2007; 28: 3074–3082.10.1016/j.biomaterials.2007.03.013Search in Google Scholar

[2] Baraton MI, Chen X, Gonsalves KE. Ftir study of nanostructured alumina nitride powder surface: determination of the acidic/basic sites by CO, CO2, and acetic acid adsorptions. Nanostruct Mater 1997; 8: 435–445.10.1016/S0965-9773(97)00189-XSearch in Google Scholar

[3] Berry CC, Campbell G, Spadiccino A, Robertson M, Curtis ASG. The influence of microscale topography on fibroblast attachment and motility. Biomaterials 2004; 25: 5781–5788.10.1016/j.biomaterials.2004.01.029Search in Google Scholar

[4] Bos R, van der Mei HC, Busscher HJ. Physico-chemistry of initial microbial adhesive interactions: its mechanisms and methods for study. FEMS Microbiol Rev 1999; 23: 179–230.10.1016/S0168-6445(99)00004-2Search in Google Scholar

[5] Brody S, Anilkumar T, Liliensiek S, et al. Characterizing nanoscale topography of the aortic heart valve basement membrane for tissue engineering heart valve scaffold design. Tissue Eng 2006; 12: 413–421.10.1089/ten.2006.12.413Search in Google Scholar

[6] Curtis ASG, Dalby M, Gadegaard N. Cell signaling arising from nanotopography: implications for nanomedical devices. Nanomdicine 2006; 1; 67–72.10.2217/17435889.1.1.67Search in Google Scholar

[7] Dalby MJ, Riehle MO, Johnstone H, Affrossman S, Curtis ASG. In vitro reaction of endothelial cells to polymer demixed nanotopography. Biomaterials 2002; 23: 2945–2954.10.1016/S0142-9612(01)00424-0Search in Google Scholar

[8] Delgado-Ruiz RA, Calvo-Guirado JL, Abboud M, et al. Porous titanium granules in critical size defects of rabbit tibia with or without membranes. Int J Oral Sci 2014; 6: 105–110.10.1038/ijos.2014.6Search in Google Scholar PubMed PubMed Central

[9] Fujita S, Ohshima M, Iwata H. Time-lapse observation of cell alignment on nanogrooved patterns. J R Soc Interface 2009; 6: 269–277.10.1098/rsif.2008.0428.focusSearch in Google Scholar PubMed PubMed Central

[10] Habibovic P, Yuan H, van den Doel M, et al. Relevance of osteoinductive biomaterials in critical-sized orthotopic defect. J Orthop Res 2006; 24: 867–876.10.1002/jor.20115Search in Google Scholar PubMed

[11] Hadrich A, Lautié A, Mhiri T. Vibrational study and fluorescence bands in the FT-Raman spectra of Ca(10-x)Pb(x)(PO4)6(OH)2 compounds. Spectrochim Acta A 2001; 57: 1673–1681.10.1016/S1386-1425(01)00402-4Search in Google Scholar

[12] Han J, Zheng S. Organic-inorganic hybrid brushes consisting of macrocyclic oligomeric silsesquioxane and poly(ε-caprolactone): synthesis, characterization, and supramolecular inclusion complexation with α-cyclodextrin. J Polym Sci A Pol Chem 2009; 47: 6894–6907.10.1002/pola.23729Search in Google Scholar

[13] Harrison RG. On the stereotropism of embryonic cells. Science 1911; 34: 279–281.10.1126/science.34.870.279Search in Google Scholar

[14] Karuri NW, Liliensiek S, Teixeira AI, et al. Biological length scale topography enhances cell-substratum adhesion of human corneal epithelial cells. J Cell Sci 2004; 117: 3153–3164.10.1242/jcs.01146Search in Google Scholar

[15] Kidambi S, Lee I, Chan C. Controlling primary hepatocyte adhesion and spreading on protein-free polyelectrolyte multilayer films. J Am Chem Soc 2004; 126: 16286–16287.10.1021/ja046188uSearch in Google Scholar

[16] Klabunde KJ, Strak J, Koper O, et al. Nanocrystals as stoichiometric reagents with unique surface chemistry. J Phys Chem 1996; 100: 12141–12153.10.1021/jp960224xSearch in Google Scholar

[17] Liu DM, Troczynski T, Tseng WJ. Water-based sol-gel synthesis of hydroxyapatite: process development. Biomaterials 2001; 22: 1721–1730.10.1016/S0142-9612(00)00332-XSearch in Google Scholar

[18] Milner KR, Siedlecki CA. Submicron poly(L-lactic acid) pillars affect fibroblast adhesion and proliferation. J Biomed Mater Res A 2007; 82A: 80–91.10.1002/jbm.a.31049Search in Google Scholar PubMed

[19] Parent CA, Devreotes PN. A cell’s sense of direction. Science 1999; 284: 765–770.10.1126/science.284.5415.765Search in Google Scholar PubMed

[20] Park MR, Banks MK, Applegate B, et al. Influence of nanophase titania topography on bacterial attachment and metabolism. Int J Nanomedicine 2008; 3: 497–504.Search in Google Scholar

[21] Puckett SD, Taylor E, Raimondo T, et al. The relationship between the nanostructure of titanium surfaces and bacterial attachment. Biomaterials 2010; 31: 706–713.10.1016/j.biomaterials.2009.09.081Search in Google Scholar PubMed

[22] Rimondini L, Farè S, Brambilla E, et al. The effect of surface roughness on early in vivo plaque colonization on titanium. J Periodontol 1997; 68: 556–562.10.1902/jop.1997.68.6.556Search in Google Scholar

[23] Spatz JP, Geige B. Environmental sensing through focal adhesions. Method Cell Biol 2007; 83: 89–111.10.1016/S0091-679X(07)83005-6Search in Google Scholar

[24] Stanto MM, Rankenberg JM, Park BW, McGimpsey WG, Malcuit C, Lambert CR. Cell behavior on surface modified polydimethylsiloxane (PDMS). Macromol Biosci 2014; 14: 953–964.10.1002/mabi.201300504Search in Google Scholar

[25] Turner AMP, Dowell N, Turner SWP, et al. Attachment of astroglial cells to microfabricated pillar arrays of different geometries. J Biomed Mater Res 2000; 51: 430–441.10.1002/1097-4636(20000905)51:3<430::AID-JBM18>3.0.CO;2-CSearch in Google Scholar

[26] Wan YQ, Wang Y, Liu ZM, et al. Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(l-lactide). Biomaterials 2005; 26: 4453–4459.10.1016/j.biomaterials.2004.11.016Search in Google Scholar

[27] Wilkinson CDW, Riehle M, Wood M, Gallagher J, Curtis SG. The use of materials patterned on a nano- and micro-metric scale in cellular engineering. Mater Sci Eng C 2002; 19: 263–269.10.1016/S0928-4931(01)00396-4Search in Google Scholar

[28] Wu SJ, De Jonghe LC, Rahaman MN. Sintering of nanophase g-Al2O3 powder. J Am Ceram Soc 1996; 79: 2207–2211.10.1111/j.1151-2916.1996.tb08962.xSearch in Google Scholar

Received: 2015-4-21
Accepted: 2015-11-9
Published Online: 2015-12-18
Published in Print: 2016-10-1

©2016 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 25.11.2025 from https://www.degruyterbrill.com/document/doi/10.1515/bmt-2015-0075/html
Scroll to top button