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Effects of laser processing conditions on wettability and proliferation of Saos-2 cells on CoCrMo alloy surfaces

  • Afif Batal

    PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

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    , Aleksandra Michalek

    PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

    , Antonio Garcia-Giron

    PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

    , Vahid Nasrollahi

    PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

    ORCID logo
    , Pavel Penchev

    Research fellow part of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

    , Rachel Sammons

    Rachel Sammons is the Programme Lead for the BMedSc (Biomedical Materials Science) programme at the University of Birmingham. She is also the First Year Tutor and Examinations Officer for this Programme. Rachel Sammons research interests focus on surface interactions between mammalian cells and bacteria and biomaterials – especially titanium and hydroxyapatite, for bone repair and the prevention of infection.

    and Stefan Dimov

    Stefan Dimov is a Professor of micro manufacturing at the University of Birmingham. His research interests encompass the broad area of advanced manufacturing with a special focus on micro and nano manufacturing, additive manufacturing and hybrid manufacturing technologies. He established the micro manufacturing and hybrid manufacturing labs which are now widely recognised for their internationally leading research. His academic output includes more than 250 technical papers and 13 books. He has supervised over 20 PhD theses to completion. He has won in excess of £30M in external research grants and contracts.

Published/Copyright: November 14, 2019
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Abstract

Any processing disturbances in laser surface texturing (LST) could compromise the resulting surface topography and their desired functional response. Disturbances such as focal plane offsets and beam incident angle variations are always present in LST processing of 3D parts and can affect the surface morphology. In this research the effects of these laser processing disturbances in producing laser induced surface structures (LIPSS) on CoCrMo alloy substrates were investigated. In particular, these two disturbances were considered as laser processing variables to determine their effects on functional responses of LIPSS treated surfaces, i.e. surface wettability and the proliferation of Saos-2 osteoblast-like cells were evaluated. It was found that the changes of laser processing conditions led to a decrease in surface wettability and Saos-2 cells proliferation. In addition, a correlation between surface wettability and cell proliferation on LIPSS treated surface was identified and conclusions made about the effects of investigated process disturbances on the functional response of LIPSS treated CoCrMo substrates.

About the authors

Afif Batal

PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

Aleksandra Michalek

PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

Antonio Garcia-Giron

PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

Vahid Nasrollahi

PhD student of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

Pavel Penchev

Research fellow part of the laser processing group in the Department of Mechanical Engineering at the University of Birmingham, UK.

Rachel Sammons

Rachel Sammons is the Programme Lead for the BMedSc (Biomedical Materials Science) programme at the University of Birmingham. She is also the First Year Tutor and Examinations Officer for this Programme. Rachel Sammons research interests focus on surface interactions between mammalian cells and bacteria and biomaterials – especially titanium and hydroxyapatite, for bone repair and the prevention of infection.

Stefan Dimov

Stefan Dimov is a Professor of micro manufacturing at the University of Birmingham. His research interests encompass the broad area of advanced manufacturing with a special focus on micro and nano manufacturing, additive manufacturing and hybrid manufacturing technologies. He established the micro manufacturing and hybrid manufacturing labs which are now widely recognised for their internationally leading research. His academic output includes more than 250 technical papers and 13 books. He has supervised over 20 PhD theses to completion. He has won in excess of £30M in external research grants and contracts.

Acknowledgments

The research was supported by two European Commission H2020 projects, the ITN programme ‘European ESRs Network on Short Pulsed Laser Micro/Nanostructuring of Surfaces for Improved Functional Applications’ (Laser4Fun) and the FoF programme ‘High-Impact Injection Moulding Platform for mass-production of 3D and/or large micro-structured surfaces with Antimicrobial, Self-cleaning, Anti-scratch, Anti-squeak and Aesthetic functionalities’ (HIMALAIA). In addition, the work was carried out within the framework of the UKIERI DST programme ‘Surface functionalisation for food, packaging, and healthcare applications’.

  1. Author Contributions

  2. A. Batal

  3. Main author, conducted the laser experiments and functional tests.

  4. A. Michalek

  5. Assisted with LIPSS optimization and characterization.

  6. A. Garcia-Giron

  7. Assisted with sample preparation and wettability analysis.

  8. V. Nasrollahi

  9. Assisted with SEM imaging.

  10. P. Penchev

  11. Assisted with laser machining setup and supervision.

  12. R. Sammons

  13. Supervision and proofreading.

  14. S. Dimov

  15. Supervision and proofreading.

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Received: 2019-09-02
Accepted: 2019-10-19
Published Online: 2019-11-14
Published in Print: 2020-02-25

©2020 THOSS Media & De Gruyter, Berlin/Boston

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