Fetal brain injury in experimental intrauterine asphyxia and inflammation in Göttingen minipigs
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Kristin Lyng
Abstract
Objective: To examine fetal brain injury in the Göttingen minipig following intrauterine asphyxia and infection/inflammation induced at 3/4 of gestational length.
Methods: We performed laparotomy after anesthesia in six pregnant sows. We randomized 29 fetuses to one of four groups: pretreatment with saline or endotoxin followed by 30 min of umbilical cord occlusion or no occlusion. After 48 h we performed a re-laparotomy and examined the fetal brains.
Results: After total asphyxia, brain stem injury was present in the group pretreated with saline (P<0.01 vs. controls) and with endotoxin (P<0.005 vs. controls). Microglia activation was more marked in the brain stem (P<0.05) and posterior white matter (P<0.05) in the asphyxia group than in controls. Two of five fetuses in the asphyxia group had white matter injury, while no white matter lesions were found in the asphyxia/inflammation or endotoxin only groups.
Conclusions: In this Göttingen minipig model, a species closer to humans than animals commonly used in experimental studies of perinatal brain injuries, intrauterine asphyxia following pretreatment with saline caused brain stem and white matter injury. This model can be further developed to study the impact of other intrauterine exposures on brain injury.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
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Articles in the same Issue
- The chemical erosion of human health: adverse environmental exposure and in-utero pollution – determinants of congenital disorders and chronic disease
- Cesarean section in term breech presentations: do rates of adverse neonatal outcomes differ by hospital birth volume?
- Antibiotic therapy for preterm premature rupture of membranes – results of a multicenter study
- Relation between grief and subsequent pregnancy status 13 months after perinatal bereavement
- Vaginal birth after cesarean section: X-ray pelvimetry at term is informative
- Vaginal birth after cesarean section: X-ray pelvimetry at term is informative
- Dilatation of the abdominal umbilical vein is associated with increased risk of thrombotic complications
- Retrospective diagnosis of hypoxic myocardial injury in premature newborns
- Fetal brain injury in experimental intrauterine asphyxia and inflammation in Göttingen minipigs
- Intrauterine growth restriction induces increased capillary density and accelerated type I fiber maturation in newborn pig skeletal muscles
- Closing arguments for gastroschisis: management with silo reduction
- Mean platelet and red blood cell volume measurements to estimate the severity of hypertension in pregnancy
- Torsion of a pedunculated accessory hepatic lobe: differential diagnosis of projectile vomiting in a neonate
- Massive fetomaternal hemorrhage following failed external cephalic version: case report
- Resolution of peripheral tissue ischemia secondary to arterial vasospasm following treatment with a topical nitroglycerin device in two newborns: case reports
- Echocardiography and N-terminal pro BNP
- Frontal-dominant white matter lesions following congenital rubella and cytomegalovirus infection
- Perinatal Programming
- Congress Calendar
