Multiplexed testing in the autoimmunity laboratory
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José M. González-Buitrago
Abstract
A variety of technologies for autoantibody profiling have been developed. The main techniques are line-blot immunoassays, bar-coded nanoparticle immunoassays, and bead-based assays with flow cytometry detection and antigen microarrays. Some of these technologies are only able to measure a limited number of autoantibodies, while others can detect elevated numbers. Assays for antinuclear antibody specificities using line-blot immunoassays and bead-based assays with flow cytometry detection are already commercialised. Antigen microarrays for autoantibody measurement are only in the development phase, although in the not too distant future these assays will probably appear on the market. Multiplexed testing in the autoimmunity laboratory appears to have a promising future, since this technique permits a reduction in analytical time, with a shorter turnaround time.
Clin Chem Lab Med 2006;44:1169–74.
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©2006 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Multiplexed testing in the autoimmunity laboratory
- Uncertainty calculation for calibrators and controls of laboratory diagnostic assays
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- Frequency of a single nucleotide (A2317G) and 56-bp variable number of tandem repeat polymorphisms within the deoxyribonuclease I gene in five ethnic populations
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- Apolipoprotein B gene 3′VNTR polymorphism: association with plasma lipids and coronary heart disease in Han Chinese
- Paraoxonase-1 (PON1) activity, but not PON1Q192R phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population
- A modified method of prothrombin time/International Normalised Ratio determination in capillary blood and monitoring oral anticoagulant therapy
- Inhibition of the ubiquitin-proteasome system: a new avenue for atherosclerosis
- Lipid peroxidation and antioxidative status in β-thalassemia major patients with or without hepatitis C virus infection
- Role of serum S100B as a predictive marker of fatal outcome following isolated severe head injury or multitrauma in males
- Preanalytical and analytical variation of surface-enhanced laser desorption-ionization time-of-flight mass spectrometry of human serum
- Effect of nitrogen mustard, a vesicant agent, on lymphocyte energy metabolism
- Partitioning reference values of several Gaussian subpopulations with unequal prevalence – a procedure with computer program support
- Biovariability of plasma adiponectin
- Multicentre evaluation of a new point-of-care test for the determination of NT-proBNP in whole blood
- Innotrac Aio!: a point-of-care or a routine analyzer? Analytical performance and plasma/whole blood comparison
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