Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry
-
Michael Vogeser
Abstract
In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isobaric with ions commonly used for the detection of cyclosporin A and cyclosporin D, respectively. In this article it is demonstrated that limited chromatography with co-elution of such metabolites together with cyclosporin A and cyclosporin D can lead to incorrect results.
References
1 Vogeser M, Fleischer C, Meiser B, Groetzner J, Spöhrer U, Seidel D. Quantification of sirolimus by liquid chromatography-tandem mass spectrometry using on-line solid-phase extraction. Clin Chem Lab Med 2002; 40: 40–5. 10.1515/CCLM.2002.008Search in Google Scholar
2 Volosov A, Napoli KL, Soldin SJ. Simultaneous simple and fast quantification of three major immunosuppressants by liquid chromatography-tandem mass-spectrometry. Clin Biochem 2001; 34: 285–90. 10.1016/S0009-9120(01)00235-1Search in Google Scholar
3 Keevil BG, Tierney DP, Cooper DP, Morris MR. Rapid liquid chromatography-tandem mass spectrometry method for routine analysis of cyclosporin A over an extended concentration range. Clin Chem 2002; 48: 69–76. 10.1093/clinchem/48.1.69Search in Google Scholar
4 Ceglarek U, Lembcke J, Fiedler GM, Werner M, Witzigmann H, Hauss JP, et al. Rapid simultaneous quantification of immunosuppressants in transplant patients by turbulent flow chromatography combined with tandem mass spectrometry. Clin Chim Acta 2004; 346: 181–90. 10.1016/j.cccn.2004.03.017Search in Google Scholar
5 Koal T, Deters M, Casetta B, Kaever V. Simultaneous determination of four immunosuppressants by means of high speed and robust on-line solid phase extraction-high performance liquid chromatography-tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 2004; 805: 215–22. 10.1016/j.jchromb.2004.02.040Search in Google Scholar
6 Tuominen J, Suortti T, Ishikawa K, Lundell J, Koga Y. Separation of cyclosporins by high-performance liquid chromatography and mass spectrometric study of cyclosporin metabolites. Rapid Commun Mass Spectrom 1998; 12: 1085–91. 10.1002/(SICI)1097-0231(19980831)12:16<1085::AID-RCM284>3.0.CO;2-LSearch in Google Scholar
7 Streit F, Armstrong VW, Oellerich M. Mass interferences in quantification of cyclosporine using tandem mass spectrometry without chromatography [abstract]. Ther Drug Monit 2003; 25: 506Search in Google Scholar
8 Streit F, Armstrong VW, Oellerich M. Rapid liquid chromatography-tandem mass spectrometry routine method for simultaneous determination of sirolimus, everolimus, tacrolimus, and cyclosporin A in whole blood. Clin Chem 2002; 48: 955–8. 10.1093/clinchem/48.6.955Search in Google Scholar
9 Vogeser M, Zachoval R, Spöhrer U, Jacob K. Potential lack of specificity using electrospray tandem-mass spectrometry for the analysis of mycophenolic acid in serum. Ther Drug Monit 2001; 23: 722–4. 10.1097/00007691-200112000-00021Search in Google Scholar
© by Walter de Gruyter Berlin New York
Articles in the same Issue
- Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent
- Antibody titer against malondialdehyde-modified LDL compares with HDL cholesterol concentration in identifying angiographically verified coronary artery disease. Comparison of tests by ROC analysis
- Fucosylation of serum glycoproteins in lung cancer patients
- Association of cytochrome P450 2E1 genetic polymorphisms with squamous cell carcinoma of the oesophagus
- α-Tocopherol as an antiretroviral therapy supplement for HIV-1-infected patients for increased lymphocyte viability
- The effect of antioxidant supplementation on superoxide dismutase activity, Cu and Zn levels, and total antioxidant status in erythrocytes of patients with Graves' disease
- Concentrations of calcium, copper, iron, magnesium, potassium, sodium and zinc in adult female hair with different body mass indexes in Taiwan
- Estimation of the limit of detection with a bootstrap-derived standard error by a partly non-parametric approach. Application to HPLC drug assays
- Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry
- How accurate are clinical activity indices for scoring of disease activity in inflammatory bowel disease (IBD)?
- An iterative method for improved estimation of the mean of peer-group distributions in proficiency testing
- Impact of a common CV evaluation scheme on overall laboratory performance: 8-year experience of a large national proficiency testing program in Japan
- Significance of small dense low-density lipoproteins as coronary risk factor in diabetic and non-diabetic Korean populations
- Evaluation of a rapid bedside test for the quantitative determination of C-reactive protein
- Power function of the reference change value in relation to cut-off points, reference intervals and index of individuality
- Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay
- Vitamin C and aberrant electrolyte results
- POX-ACT assay and d-ROMs test: comparison impossible
- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
Articles in the same Issue
- Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent
- Antibody titer against malondialdehyde-modified LDL compares with HDL cholesterol concentration in identifying angiographically verified coronary artery disease. Comparison of tests by ROC analysis
- Fucosylation of serum glycoproteins in lung cancer patients
- Association of cytochrome P450 2E1 genetic polymorphisms with squamous cell carcinoma of the oesophagus
- α-Tocopherol as an antiretroviral therapy supplement for HIV-1-infected patients for increased lymphocyte viability
- The effect of antioxidant supplementation on superoxide dismutase activity, Cu and Zn levels, and total antioxidant status in erythrocytes of patients with Graves' disease
- Concentrations of calcium, copper, iron, magnesium, potassium, sodium and zinc in adult female hair with different body mass indexes in Taiwan
- Estimation of the limit of detection with a bootstrap-derived standard error by a partly non-parametric approach. Application to HPLC drug assays
- Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry
- How accurate are clinical activity indices for scoring of disease activity in inflammatory bowel disease (IBD)?
- An iterative method for improved estimation of the mean of peer-group distributions in proficiency testing
- Impact of a common CV evaluation scheme on overall laboratory performance: 8-year experience of a large national proficiency testing program in Japan
- Significance of small dense low-density lipoproteins as coronary risk factor in diabetic and non-diabetic Korean populations
- Evaluation of a rapid bedside test for the quantitative determination of C-reactive protein
- Power function of the reference change value in relation to cut-off points, reference intervals and index of individuality
- Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay
- Vitamin C and aberrant electrolyte results
- POX-ACT assay and d-ROMs test: comparison impossible
- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
