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Soft lithography detects partial mechano-sensoric blindness to micrometre topography in cultured aged and diseased cells

  • Dieter Kaufmann , Yi Su , Sarah Biela , Simon Jungbauer , Joachim P. Spatz and Ralf Kemkemer
Published/Copyright: June 11, 2013
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International Journal of Materials Research
From the journal International Journal of Materials Research Volume 102 Issue 7

Abstract

Cells sense their physical environment and translate mechanical forces or geometries into biochemical signals enabling them to adapt to cues in their surroundings. In this in-vitro study, cell responses to surface topography were investigated in human fibroblasts from differently old donors and in fibroblasts from patients with a common tumour-associated genetic disorder, Neurofibromatosis 1 (NF1). As a response indicator we systematically examined the mean cell orientation along micro-structured grooves with heights of 100 and 200 nm. Fibroblasts from aged donors (> 30 years) and NF1 patients were significantly less affected by the topography than those from young and healthy donors (< 30 years). The results suggest that mechano-transduction can be altered by ageing and genetic disease resulting in a partial mechano-sensoric cellular blindness.

Keywords: Soft lithography; Topography; Contact guidance; Ageing; Neurofibromatosis Type 1
* Correspondence address Dr. R. Kemkemer Max Planck Institute for Intelligent Systems (formerly Max Planck Institute for Metals Research) Heisenbergstr. 3, D-70569 Stuttgart, Germany Tel.: +49 711 689 3516 E-mail:

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Received: 2011-2-2
Accepted: 2011-4-15
Published Online: 2013-06-11
Published in Print: 2011-07-01

© 2011, Carl Hanser Verlag, München

Articles in the same Issue

  1. Contents
  2. Contents
  3. Editorial
  4. Max Planck Institute for Metals Research – 90 Years of Excellence in Materials Science
  5. Words of Welcome
  6. Words of Welcome
  7. Feature
  8. Magnetism studied with circularly polarized X-rays
  9. Solid state phase transformation kinetics: Evaluation of the modular transformation model
  10. Intelligent induction of active biosystem responses at interfaces
  11. Empirical Inference
  12. The Stuttgart Center for Electron Microscopy at the Max Planck Institute for Metals Research
  13. The filamentary growth of metals
  14. Kinetics of interface-controlled phase transformations: atomistic and mesoscopic simulations
  15. Breakthroughs in understanding elastic grain interaction and whisker formation made possible by advances in X-ray powder diffraction
  16. Ordered and disordered states in NiAs/Ni2In-type Ni1+δSn: Crystallography and order formation
  17. Of fluctuations and cross-correlations: finding order in disorder
  18. Experimental characterization of tumor spheroids for studies of the energetics of tumor growth
  19. Soft lithography detects partial mechano-sensoric blindness to micrometre topography in cultured aged and diseased cells
  20. The sweet coat of living cells – from supramolecular structure and dynamics to biological function
  21. Nonlinear spectroscopy of bio-interfaces
  22. Dedicated beamlines for in-situ investigations of materials in reduced dimensions
  23. People
  24. Laudationes
  25. Review
  26. Book review
  27. DGM News
  28. DGM News
https://doi.org/10.3139/146.110533
Keywords for this article
Soft lithography; Topography; Contact guidance; Ageing; Neurofibromatosis Type 1

Articles in the same Issue

  1. Contents
  2. Contents
  3. Editorial
  4. Max Planck Institute for Metals Research – 90 Years of Excellence in Materials Science
  5. Words of Welcome
  6. Words of Welcome
  7. Feature
  8. Magnetism studied with circularly polarized X-rays
  9. Solid state phase transformation kinetics: Evaluation of the modular transformation model
  10. Intelligent induction of active biosystem responses at interfaces
  11. Empirical Inference
  12. The Stuttgart Center for Electron Microscopy at the Max Planck Institute for Metals Research
  13. The filamentary growth of metals
  14. Kinetics of interface-controlled phase transformations: atomistic and mesoscopic simulations
  15. Breakthroughs in understanding elastic grain interaction and whisker formation made possible by advances in X-ray powder diffraction
  16. Ordered and disordered states in NiAs/Ni2In-type Ni1+δSn: Crystallography and order formation
  17. Of fluctuations and cross-correlations: finding order in disorder
  18. Experimental characterization of tumor spheroids for studies of the energetics of tumor growth
  19. Soft lithography detects partial mechano-sensoric blindness to micrometre topography in cultured aged and diseased cells
  20. The sweet coat of living cells – from supramolecular structure and dynamics to biological function
  21. Nonlinear spectroscopy of bio-interfaces
  22. Dedicated beamlines for in-situ investigations of materials in reduced dimensions
  23. People
  24. Laudationes
  25. Review
  26. Book review
  27. DGM News
  28. DGM News
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