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Eco-favourable Mobile Phase in Thin Layer Chromatographic Analysis of Surfactants: Resolution of Coexisting Alkyl Dimethylbenzyl Ammonium Chloride, Cetyltrimethyl Ammonium Bromide and Triton X 100

  • Ali Mohammad and Rizwana Mobin
Published/Copyright: September 15, 2015
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Tenside Surfactants Detergents
From the journal Tenside Surfactants Detergents Volume 52 Issue 5

Abstract

A thin-layer chromatographic system comprising of silica gel as stationary phase and a mixture of methanol, 0.1 % aqueous sodium thiocyanate, acetone and ethyl acetate in 7 : 3 : 2 : 3 ratio as eco-favourable mobile phase has been found most efficient for the separation of ternary mixture of surfactants (alkyldimethylbenzylammonium chloride (ADBAC) + cetyltrimethylammonium bromide (CTAB) + Triton X 100). The separation pattern has been presented as densitogram. Chromatographic parameters like ΔRF, separation factor (α) and resolution (RS) for the separation have been calculated. The effect of the presence of foreign substances such as metal cations, metal anions, amino acids and vitamins as impurities on the separation has been examined. The mobility trend of the separated surfactants was also examined by replacing methanol with other alcohols. The detection limits for ADBAC, CTAB and Triton X 100 have been determined. The proposed method has been successfully applied for the identification of ADBAC in the household cleaning product “Lizol”.

Kurzfassung

Für die Trennung ternärer Tensid-Mischungen (Alkyldimethylbenzylammoniumchlorid (ADBAC) + Cetyltrimethylammoniumbromid (CTAB) + Triton X 100) wurde ein sehr effizientes dünnschichtchromatographisches System bestehend aus Kieselsäuregel als stationäre Phase und der Mischung aus Methanol, wässriger Natriumthio-cyanat-lösung (0.1 %), Aceton und Ethylacetat im Verhältnis 7 : 3 : 2 : 3 als umweltfreundliche mobile Phase gefunden. Das Trennungsbild wurde als Densitogramm aufgezeichnet. Die chromatographischen Größen wie ΔRF, der Trennungsfaktor (α) und die Auflösung der Trennung (RS) wurden berechnet. Der Einfluss anwesender Fremdsubstanzen wie Metallkationen, Metallanionen, Aminosäuren und Vitamine auf die Trennung wurde untersucht. Der Mobilitätsverlauf der getrennten Tenside wurde bestimmt, indem das Methanol gegen andere Alkohole ersetzt wurde. Die Detektionsgrenzen für ADBAC, CTAB Triton X 100 wurden bestimmt. Das Verfahren wurde erfolgreich zur Identifikation von ADBAC in dem Haushaltsreiniger „Lizol“ eingesetzt.

Key words: Surfactants; thin-layer chromatography; separation; densitogram; Lizol
Stichwörter: Dünnschichtchromatographie; Trennung; Densitogramm; Lizol
*Correspondence address, Dr. Ali Mohammad, Ms. Rizwana Mobin, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh-202002, Tel.: +91-5 71-2 70 09 20, Extn. −30 02, +91-98 97 78 54 25 (Cell), E-Mail: ,

Dr. Ali Mohammad is presently working as the UGC-BSR Faculty Fellow in the Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, India after serving as professor and Chairman in the same department accumulating about 35 years teaching and research experience. Dr. Mohammad obtained his M. Sc. (1972), M.Phil. (1975), Ph. D. (1978) and D.Sc. (1996) degrees from Aligarh Muslim University, Aligarh, India. After completion of Ph. D. degree, Dr. Ali joined the Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh as lecturer, later on became reader (associate professor) in 1990 and promoted to the post of Professor in 1998. His scientific interests include physicoanalytical aspects of solid state reactions, micellar thin layer chromatography, surfactants analysis and green chromatography. He has supervised 55 students for Ph. D./M.Phil./M.Tech. degrees. He is an author or a coauthor of over 235 scientific papers and book chapters in the area of chromatography. A book in two volumes on Green Solvents (Edited with Dr. Inamuddin) has been published in 2012 by Springer publisher. Dr. Mohammad has served as editor of Journal, Chemical and Environmental Research published from India since 1992 till 2010. He has also served as the Associate Editor for Analytical Chemistry section of Journal of Indian Chemical Society and presently on the editorial boards of Acta Chromatographica, Acta Universitatis Cibiniensis Seria F. Chemia and Annals of Agrarian Science. Recently he has been nominated as Visiting Professor to King Saud University, Riyadh, Saudi Arabia. Prof. Mohammad has attended several national and international conferences, delivered lectures and chaired sessions. He is the member of many scientific bodies, Ph. D. thesis adjudicator in various universities and reviewer of several foreign and Indian journals.

Rizwana Mobin is a research scholar at Department of Applied Chemistry, Aligarh Muslim University, working currently on Ph. D. entitled “Studies on thin layer chromatographic analysis of surfactants”. She has completed her Graduation and -Masters in Industrial Chemistry from A.M.U., Aligarh. She has been gold medalist in Masters (2011). She can be mailed at .

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Received: 2014-07-11
Accepted: 2014-10-29
Published Online: 2015-09-15
Published in Print: 2015-09-15

© 2015, Carl Hanser Publisher, Munich

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  1. Contents/Inhalt
  2. Contents
  3. Review Article
  4. How to Improve Sustainability and Environmentally Friendly Behaviour in Automatic Dishwashing? Example: Germany
  5. Physical Chemistry
  6. αs-Casein-PE6400 Mixtures: Thermodynamics of Micelle Formation
  7. Effect of Alcohols on Phase Stability of Ionic Liquid Microemulsions
  8. The Effect on Solution Properties of Replacing a Hydrogen Atom with a Methyl Group in a Surfactant
  9. Application
  10. New Approach of Sucrose Myristate as a Promising Penetration Enhancer in Dermal Preparations
  11. Environmental Chemistry
  12. Identification of Non-ionic Surfactants in Elements of the Aquatic Environment
  13. Synthesis
  14. Synthesis and Properties of 1,2,3,4-Butanetetracarboxylic Acid Type Gemini Surfactants with Semifluoroalkyl Chain as Hydrophobic Group
  15. Solvent Free Synthesis of Four Cationic Surfactants: Evaluation of Their Corrosion Inhibition Properties on Zinc Phosphate Conversion Coating on Mild Steel
  16. Synthesis and Spectrophotometric Study of Toxic Metals Extraction by Novel Thio-Based Non-Ionic Surfactant
  17. Analysis
  18. Eco-favourable Mobile Phase in Thin Layer Chromatographic Analysis of Surfactants: Resolution of Coexisting Alkyl Dimethylbenzyl Ammonium Chloride, Cetyltrimethyl Ammonium Bromide and Triton X 100
https://doi.org/10.3139/113.110393
Keywords for this article
Surfactants; thin-layer chromatography; separation; densitogram; Lizol

Articles in the same Issue

  1. Contents/Inhalt
  2. Contents
  3. Review Article
  4. How to Improve Sustainability and Environmentally Friendly Behaviour in Automatic Dishwashing? Example: Germany
  5. Physical Chemistry
  6. αs-Casein-PE6400 Mixtures: Thermodynamics of Micelle Formation
  7. Effect of Alcohols on Phase Stability of Ionic Liquid Microemulsions
  8. The Effect on Solution Properties of Replacing a Hydrogen Atom with a Methyl Group in a Surfactant
  9. Application
  10. New Approach of Sucrose Myristate as a Promising Penetration Enhancer in Dermal Preparations
  11. Environmental Chemistry
  12. Identification of Non-ionic Surfactants in Elements of the Aquatic Environment
  13. Synthesis
  14. Synthesis and Properties of 1,2,3,4-Butanetetracarboxylic Acid Type Gemini Surfactants with Semifluoroalkyl Chain as Hydrophobic Group
  15. Solvent Free Synthesis of Four Cationic Surfactants: Evaluation of Their Corrosion Inhibition Properties on Zinc Phosphate Conversion Coating on Mild Steel
  16. Synthesis and Spectrophotometric Study of Toxic Metals Extraction by Novel Thio-Based Non-Ionic Surfactant
  17. Analysis
  18. Eco-favourable Mobile Phase in Thin Layer Chromatographic Analysis of Surfactants: Resolution of Coexisting Alkyl Dimethylbenzyl Ammonium Chloride, Cetyltrimethyl Ammonium Bromide and Triton X 100
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