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A Fluorometric Fullerenol Sensor for Rapid Detection of Ionic and Non-Ionic Surfactants

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Published/Copyright: April 5, 2013
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Tenside Surfactants Detergents
From the journal Tenside Surfactants Detergents Volume 47 Issue 1

Abstract

A fullerenol sensor utilizing fluorescence quenching of a labeled protein was developed for rapid detection and quantification of ionic (cetyltriammonium bromide (CTAB), sodium dodecyl sulphate, (SDS)) and non-ionic (Tween 20 and Triton X-100) surfactants in solution. The sensor is based on the competitive adsorption of the surfactants and the fluorescently labeled protein to fullerenols – hydroxylated buckminsterfullerenes. The fluorescence quenching between fullerenols and the fluorochrome-labeled protein was detected and related to the replacement of the labeled protein by the surfactants. The non-ionic surfactants could be measured at sub to low micromolar concentrations whereas significantly higher concentration of ionic surfactants was required to efficiently cover the fullerenol surface. The combination of the novel fullerenol sensor with existing micro titer plate fluorometric instrumentation can find use as a versatile and rapid probe for tracing various types of surface active molecules.

Kurzfassung

Ein Fullerenol Sensor, der die Fluoreszenzlöschung markierter Proteine nutzt, wurde für den Schnellnachweis und die Quantifizierung von ionischen (Cetyltriammoniumborimid [CTAB] und Natriumdodecylsulfat [NaDS]) und von nicht-ionischen Tensiden (Tween 20 und Triton X-100) in Lösung entwickelt. Der Sensor beruht auf der Konkurrenzadsorption der Tenside und der fluoreszenzmarkierten Proteine an die Fullerenole – die hydroxylierten Buckminsterfullerene. Die nicht-ionischen Tenside können bis in den sub- und gering mikromolaren Konzentrationsbereich gemessen werden, wohingegen deutlich höhere Konzentrationen der ionischen Tenside erforderlich waren, um die Fullerenoloberfläche effizient zu bedecken. Die Kombination des neuen Fullerenolsensors mit der bereits existierenden Mikrotiterplatten-Fluoreszenzmethode kann als eine vielseitige und schnelle Methode zur Verfolgung verschiedener Typen von grenzflächenaktiven Molekülen eingesetzt werden.

Key words:: fluorometric fullerenol sensor; rapid detection; buckminsterfullerenes; cationic curfactant; anionic surfactant; nonionic surfactant; labeled protein; cetyltriammonium bromide (CTAB); sodium dodecyl sulphate (SDS); Tween 20; Triton X-100
Stichwörter:: fluorimetrischer Fullerenol-Sensor; Schnellnachweis; Buckminsterfullerene; kationisches Tensid; anionisches Tensid; nicht-ionisches Tensid; markiertes Protein; Cetyltriammoniumbromid (CTAB); Natriumdodecylsulfate (SDS); Tween 20; Triton X-100
∗ H. Härmä, Laboratory of Biophysics and Medicity, University of Turku, Tykistökatu 6A, FI-20520, Turku, Finland

Harri Härmä was born in 1966 and graduated at the University of Turku from the Department of Biotechnology. He is a group leader at the University of Turku in the Laboratory of Biophysics and his research interest is focused on assay and sensor technologies based on non-specificity, nanotechnology and fluorescence.

Susana Laakso was born in 1966. She obtained her PhD in Physiology and Biochemistry from the Lisbon Classical University in 1996. In the Laboratory of Biophysics, she works as a Senior Scientist developing non-specific binding methods.

Sari Pihlasalo was born in 1981. She obtained her master degree in physical chemistry from the University of Turku in 2006. She is a doctoral student in the Laboratory of Biophysics and her main field of research is focused on the development of quantitative assays based on fluorescence resonance energy transfer and non-specific binding.

Pekka Hänninen was born in 1962 and studied computer science and electrical engineering at the Tampere University of Technology. His university career started at Tampere and in 1988 he moved to European Molecular Biology Laboratory to develop confocal microscopic imaging. In 1992 he moved back to Finland to newly formed group: Laboratory of Biophysics at University of Turku. Since 1992 he has been developing instrumentation and methods for high-resolution microscopy, multiphoton fluorescence and bioanalytics. After his PhD thesis in 1995 he continued as a post-doc in the same group. Since 2002 he leads the laboratory of Biophysics and has been appointed as professor of Medical Physics and Engineering with research field in Biomedical imaging. His current research includes methods and instrumentation in biomedical research with special emphasis in utilisation of fluorescence.

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Received: 2009-09-05
Revised: 2009-10-30
Published Online: 2013-04-05
Published in Print: 2010-01-01

© 2010, Carl Hanser Publisher, Munich

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  1. Contents/Inhalt
  2. Contents
  3. Abstracts
  4. Abstracts
  5. Review
  6. Development of Cleavable Surfactants
  7. Environmental Chemistry
  8. Removal of Tartrazine from Aqueous Solution by Activated Coal
  9. Novel Surfactants
  10. A Process for the Production of Φ-Sulfo Fatty Methyl Ester Sulfonates (Φ-MES)
  11. Synthesis of 3-Dehydroabietylamino-2-Hydroxypropyl Trimethylammonium Chloride and its Antibacterial Activity
  12. Physical Chemistry
  13. Octanol-Water Partition Coefficients of Surfactants: Slow Stirring/Surface Tension Method
  14. Synthesis
  15. Synthesis and Properties Evaluation of Sodium Fatty Alcohol Polyoxyethylene Ether Sulfonate
  16. Short Communication
  17. A Fluorometric Fullerenol Sensor for Rapid Detection of Ionic and Non-Ionic Surfactants
  18. GDCh-News
  19. Fifth European Detergents Conference Report
https://doi.org/10.3139/113.110052
Keywords for this article
fluorometric fullerenol sensor; rapid detection; buckminsterfullerenes; cationic curfactant; anionic surfactant; nonionic surfactant; labeled protein; cetyltriammonium bromide (CTAB); sodium dodecyl sulphate (SDS); Tween 20; Triton X-100

Articles in the same Issue

  1. Contents/Inhalt
  2. Contents
  3. Abstracts
  4. Abstracts
  5. Review
  6. Development of Cleavable Surfactants
  7. Environmental Chemistry
  8. Removal of Tartrazine from Aqueous Solution by Activated Coal
  9. Novel Surfactants
  10. A Process for the Production of Φ-Sulfo Fatty Methyl Ester Sulfonates (Φ-MES)
  11. Synthesis of 3-Dehydroabietylamino-2-Hydroxypropyl Trimethylammonium Chloride and its Antibacterial Activity
  12. Physical Chemistry
  13. Octanol-Water Partition Coefficients of Surfactants: Slow Stirring/Surface Tension Method
  14. Synthesis
  15. Synthesis and Properties Evaluation of Sodium Fatty Alcohol Polyoxyethylene Ether Sulfonate
  16. Short Communication
  17. A Fluorometric Fullerenol Sensor for Rapid Detection of Ionic and Non-Ionic Surfactants
  18. GDCh-News
  19. Fifth European Detergents Conference Report
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