Effect of Cellulase Enzyme on Cellulose Nano-topography

A. Calvimontes; M. Stamm; V. Dutschk

doi:10.3139/113.110044

Article

Effect of Cellulase Enzyme on Cellulose Nano-topography

A. Calvimontes , M. Stamm and V. Dutschk

Published/Copyright: April 5, 2013

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From the journal Tenside Surfactants Detergents Volume 46 Issue 6

Abstract

In this study, modification effects of cellulose foil by cellulase enzyme are studied in respect to topography changes by means of atomic force microscopy (AFM). The results provide useful information to understand the enzyme action in the amorphous and crystalline regions of cellulose. It was revealed, that the treatment of cellulose with the cellulase enzyme depends on its concentration, which selectively attacks amorphous cellulose regions. At lower concentration, a random surface roughening of the cellulose foil was observed. With increasing enzyme concentration, the cellulose surface became smoother. On the basis of this knowledge, a conceptual model to describe the conditioning effect by cellulase on the cleanability of cotton fabrics was developed. With the help of this model, we are able to estimate changes in the nanoporosity of cotton fibres.

Kurzfassung

Im Rahmen dieser Arbeit wurden die Effekte der Modifikation von Cellulose mit dem Enzym Cellulase auf die Topographieänderungen mittels Rasterkraftmikroskopie (AFM) untersucht. Die Ergebnisse bieten ausreichende Auskunft zum Verständnis der Enzymwirkung auf die Änderungen in amorphen und kristallinen Regionen der Cellulose. Es wurde gezeigt, dass die Modifizierung der Cellulose mit Cellulase abhängig von der Enzymkonzentration selektiv amorphe Bereiche der Cellulose angreift. Bei niedrigen Konzentrationen wurde eine zufällige Oberflächenanrauung der Cellulose-Folie beobachtet. Bei höheren Konzentrationen wird die Celluloseoberfläche glatter. Auf der Basis der gewonnenen Ergebnisse wurde ein konzeptuelles Modell zur Beschreibung des Aufbereitungseffekts der Cellulose bei Cellulase auf die Auswaschbarkeit von Baumwolle entwickelt. Unter Zuhilfenahme des Modells ist eine Bewertung von Änderungen der Nanoporosität von Baumwollfasern möglich.

Key words:: Cellulose; enzyme cellulase; Celluclean; Cellophan; topography; morphology; porosity

Stichwörter:: Cellulose; Cellulase-Enzym; Celluclean; Cellophan; Topographie; Morphologie; Porosität

^∗ Alfredo Calvimontes, Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, 01069 Dresden, Germany, E-Mail: calvimontes@ipfdd.de

M. Sc. Alfredo Calvimontes was born in 1965 and graduate from the La Salle University (Mexico), Faculty of Chemical Engineering in 1989. During twelve years he worked as a manufacturing engineer in the chemical and textile industries in Mexico and Bolivia. In 2004 he received his Master Degree in Textile and Clothing Engineering from the Dresden University of Technology. At the present time he works at the Leibniz Institute of Polymer Research Dresden.

Prof. Dr. Manfred Stamm studied Physics from 1968 to 1974 at the University of Frankfurt am Main, received his PhD at the Institute of Physical Chemistry of the University of Mainz and finalised his Habilitation on Investigation of polymer surfaces and interfaces with nanometer resolution. Between 1979 and 1985 he worked as staff scientist at Institute of Solid State Research in Jülich. Between 1984 and 1985 he was visiting scientist at Brookhaven national laboratory in the United States. Between 1985 and 1999 he was staff scientist and project leader at the Max-Planck Institute of Polymer Research in Mainz. From 1999 Manfred Stamm is professor for Physical Chemistry of Polymeric Materials at the Technical University Dresden and head of IPF-Institute of Physical Chemistry and Physics of Polymers at Leibniz Institute of Polymer Research in Dresden. Prof. Stamm is member of editorial board of Coll. Interf. Sci., referee of several journals and funding agencies, member of board of “Dresden Center of Science and Art” DZWK and of “Dresden Materials Research Science Association” MFD. In 2004 received the International Belgian Polymer Group Award.

Dr.-Ing. Victoria Dutschk was born in 1966 in Bendery (Moldova) and graduate from the Odessa State University, Faculty of Mechanics and Mathematics in 1988. She obtained her PhD in 2000 at the Technische Universität Dresden, Faculty of Mechanical Engineering, Department Material Science under the guidande of Prof. Wolfgang Pompe. Since 1994 she has been working as a research associate at the Leibniz Institute of Polymer Research Dresden in the field interfacial engineering focussing specifically on surface active agents.

References

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Received: 2009-05-19

Revised: 2009-08-18

Published Online: 2013-04-05

Published in Print: 2009-11-01

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Articles in the same Issue

https://doi.org/10.3139/113.110044

Keywords for this article

Cellulose; enzyme cellulase; Celluclean; Cellophan; topography; morphology; porosity