Home Influence of the ionic form of a cation-exchange adsorbent on chromatographic separation of galactooligosaccharides
Article
Licensed
Unlicensed Requires Authentication

Influence of the ionic form of a cation-exchange adsorbent on chromatographic separation of galactooligosaccharides

  • Juraj Moravčík EMAIL logo , Michal Gramblička , Łukasz Wiśniewski , Katarína Vaňková and Milan Polakovič
Published/Copyright: April 11, 2012
Become an author with De Gruyter Brill

Abstract

Separation performance of sodium, lithium, potassium and hydrogen ionic forms of a commercial cation exchanger, Diaion UBK 530, based on a styrene-divinylbenzene copolymer resin support was studied. The adsorbents were applied to the separation of galactooligosaccharides from their mixture with lactose, glucose and galactose. Separation performance of the hydrogen ionic form of the adsorbent was superior to that of other adsorbent forms. A much higher yield of galactooligosaccharides of the requested purity was achieved for this adsorbent form owing to its better kinetic properties. Adsorption properties of individual adsorbents were discussed in relation to possible adsorption mechanisms.

[1] Antošová, M., Polakovič, M., & Báleš, V. (1999). Separation of fructooligosaccharides on a cation-exchange HPLC column in silver form with refractometric detection. Biotechnology Techniques, 13, 889–892. DOI: 10.1023/a:1008986426849. http://dx.doi.org/10.1023/A:100898642684910.1023/A:1008986426849Search in Google Scholar

[2] Chen, C. W., Ou-Yang, C. C., & Yeh, C.W. (2003). Synthesis of galactooligosaccharides and transgalactosylation modeling in reverse micelles. Enzyme and Microbial Technology, 33, 497–507. DOI: 10.1016/s0141-0229(03)00155-8. http://dx.doi.org/10.1016/S0141-0229(03)00155-810.1016/S0141-0229(03)00155-8Search in Google Scholar

[3] Garcia, F. A. P., & Pires, E. M. V. (1993). Chromatography. In J. F. Kennedy, & M. S. Cabral (Eds.), Recovery processes for biological materials (pp. 415–452). Chichester, UK: Wiley. Search in Google Scholar

[4] Gibson, G. R., & Roberfroid, M. B. (1995). Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. Journal of Nutrition, 125, 1401–1412. 10.1093/jn/125.6.1401Search in Google Scholar

[5] Gramblička, M., & Polakovič, M. (2007). Adsorption equilibria of glucose, fructose, sucrose, and fructooligosaccharides on cation exchange resins. Journal of Chemical & Engineering Data, 52, 345–350. DOI: 10.1021/je060169d. http://dx.doi.org/10.1021/je060169d10.1021/je060169dSearch in Google Scholar

[6] Ishikawa, F., Takayama, H., Matsumoto, K., Ito, M., Chonan, O., Deguchi, Y., Kikuchi-Hayakawa, H., & Watanuki, M. (1995). Effects of β1–4 linked galacto-oligosaccharides on human fecal microflora. Bifidus-Flores, Fructus et Semina, 9, 5–18. (in Japanese) Search in Google Scholar

[7] Krishnamoorthy, C., & Desai, A. D. (1953). Kinetics of ion exchange between two adsorbents: I. Monovalent ion systems. Soil Science, 76, 307–316. http://dx.doi.org/10.1097/00010694-195310000-0000710.1097/00010694-195310000-00007Search in Google Scholar

[8] Li, Z. Y., Xiao, M., Lu, L. L., & Li, Y. M. (2008). Production of non-monosaccharide and high-purity galactooligosachcarides by immobilized enzyme catalysis and fermentation with immobilized yeast cells. Process Biochemistry, 43, 896–899. DOI: 10.1016/j.procbio.2008.04.016. http://dx.doi.org/10.1016/j.procbio.2008.04.01610.1016/j.procbio.2008.04.016Search in Google Scholar

[9] Matsumoto, K., Kobayashi, Y., Ueyama, S., Watanabe, T., Tanaka, R., Kan, T., Kuroda, A., & Sumihara, Y. (1990). Galactooligosaccharides. In T. Nakanuki (Ed.), Japanese technology reviews (pp. 90–106). Tokyo, Japan: Gordon and Breach. Search in Google Scholar

[10] Morishita, Y., Oowada, T., Ozaki, A., & Mizutani, T. (2002). Galactooligosaccharides in combination with Bifidobacterium and Bacteroides affects the population of Clostridium perfringens in the intestine of gnotobiotic mice. Nutrition Research, 22, 1333–1341. DOI: 10.1016/s0271-5317(02)00455-4. http://dx.doi.org/10.1016/S0271-5317(02)00455-410.1016/S0271-5317(02)00455-4Search in Google Scholar

[11] Sako, T., Matsumoto, K., & Tanaka, R. (1999). Recent progress on research and applications of non-digestible galactooligosaccharides. International Dairy Journal, 9, 69–80. DOI: 10.1016/s0958-6946(99)00046-1. http://dx.doi.org/10.1016/S0958-6946(99)00046-110.1016/S0958-6946(99)00046-1Search in Google Scholar

[12] Schmidt-Traub, H. (2005). Preparative chromatography. Weinheim, Germany: Wiley-VCH. http://dx.doi.org/10.1002/352760348410.1002/3527603484Search in Google Scholar

[13] Vaňková, K., Ačai, P., & Polakovič, M. (2010a). Modelling of fixed-bed adsorption of mono-, di-, and fructooligosaccharides on a cation-exchange resin. Biochemical Engineering Journal, 49, 84–88. DOI: 10.1016/j.bej.2009.11.015. 10.1016/j.bej.2009.11.015Search in Google Scholar

[14] Vaňková, K., Gramblička, M., & Polakovič, M. (2010b). Single-component and binary adsorption equilibria of fructooligosaccharides, glucose, fructose, and sucrose on a Caform cation exchanger. Journal of Chemical & Engineering Data, 55, 405–410. DOI: 10.1021/je9003965. http://dx.doi.org/10.1021/je900396510.1021/je9003965Search in Google Scholar

[15] Vaňková, K., & Polakovič, M. (2010). Optimization of singlecolumn chromatographic separation of fructooligosaccharides. Process Biochemistry, 45, 1325–1329. DOI: 10.1016/j.procbio.2010.04.025. http://dx.doi.org/10.1016/j.procbio.2010.04.02510.1016/j.procbio.2010.04.025Search in Google Scholar

[16] Vaňková, K. and Polakovič, M. (2012). Design of separation of fructooligosaccharides using simulated moving-bed chromatography. Chemical Engineering & Technology, 35, 161–168. DOI: 10.1002/ceat.201100254. http://dx.doi.org/10.1002/ceat.20110025410.1002/ceat.201100254Search in Google Scholar

[17] Vente, J. A. (2004). Adsorbent functionality in relation to selectivity and capacity in oligosaccharide separations. Ph.D. thesis, University of Twente, The Netherlands. Search in Google Scholar

Published Online: 2012-4-11
Published in Print: 2012-6-1

© 2012 Institute of Chemistry, Slovak Academy of Sciences

Downloaded on 9.9.2025 from https://www.degruyterbrill.com/document/doi/10.2478/s11696-012-0160-4/html
Scroll to top button