Continuous production of citric acid from raw glycerol by Yarrowia lipolytica in cell recycle cultivation
-
Anita Rywińska
and
Waldemar Rymowicz
Abstract
The kinetics of continuous citric acid (CA) fermentation, using two acetate-negative mutants of Yarrowia lipolytica (Wratislavia AWG7 and Wratislavia 1.31) with long-term cell recycle, were investigated at a dilution rate (D) of 0.011 h−1. The capacity of the mutants to produce CA from raw glycerol under steady state conditions was compared. The process involving the Wratislavia AWG7 strain showed that this strain was a better producer of CA than the Wratislavia 1.31 strain in this regime. In steady state, the concentration of CA in the effluent was constant and amounted to 116 g dm−3. This corresponded to a volumetric CA production rate of 1.3 g dm−3 h−1. A stable high-level production of CA was maintained for over 400 h of operation. The Wratislavia AWG7 strain employed remained highly stable throughout the experiment and demonstrated good viability, high genetic and phenotypic stability over the long-term continuous fermentation process.
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© 2010 Institute of Chemistry, Slovak Academy of Sciences
Articles in the same Issue
- Mechanisms controlling lipid accumulation and polyunsaturated fatty acid synthesis in oleaginous fungi
- Predicting retention indices of aliphatic hydrocarbons on stationary phases modified with metallocyclams using quantitative structure-retention relationships
- New SPME fibre for analysis of mequinol emitted from DVDs
- Continuous production of citric acid from raw glycerol by Yarrowia lipolytica in cell recycle cultivation
- Enhancing the production of gamma-linolenic acid in Hansenula polymorpha by fed-batch fermentation using response surface methodology
- Process characteristics for a gas—liquid system agitated in a vessel equipped with a turbine impeller and tubular baffles
- Kinetic study of pyrolysis of waste water treatment plant sludge
- Transport phenomena in an agitated vessel with an eccentrically located impeller
- Membrane extraction of 1-phenylethanol from fermentation solution
- Theoretical study on transesterification in a combined process consisting of a reactive distillation column and a pervaporation unit
- Wall effects on terminal falling velocity of spherical particles moving in a Carreau model fluid
- The effect of the physical properties of the liquid phase on the gas-liquid mass transfer coefficient in two- and three-phase agitated systems
- Effectiveness of nitric oxide ozonation
- Modelling of nanocrystalline iron nitriding process — influence of specific surface area
- Effect of CeO2 and Sb2O3 on the phase transformation and optical properties of photostable titanium dioxide
- Carnauba wax microparticles produced by melt dispersion technique
- Complexation studies of 3-substituted β-diketones with selected d- and f-metal ions
- Influence of the solvent donor number on the O/W partition ratio of Cu(II) complexes of 1,2-dialkylimidazoles
- Continuous dialysis of sulphuric acid in the presence of zinc sulphate
- Differences in affinity of arylstilbazolium derivatives to tetraplex structures
- Fast ferritin immunoassay on PDMS microchips
Articles in the same Issue
- Mechanisms controlling lipid accumulation and polyunsaturated fatty acid synthesis in oleaginous fungi
- Predicting retention indices of aliphatic hydrocarbons on stationary phases modified with metallocyclams using quantitative structure-retention relationships
- New SPME fibre for analysis of mequinol emitted from DVDs
- Continuous production of citric acid from raw glycerol by Yarrowia lipolytica in cell recycle cultivation
- Enhancing the production of gamma-linolenic acid in Hansenula polymorpha by fed-batch fermentation using response surface methodology
- Process characteristics for a gas—liquid system agitated in a vessel equipped with a turbine impeller and tubular baffles
- Kinetic study of pyrolysis of waste water treatment plant sludge
- Transport phenomena in an agitated vessel with an eccentrically located impeller
- Membrane extraction of 1-phenylethanol from fermentation solution
- Theoretical study on transesterification in a combined process consisting of a reactive distillation column and a pervaporation unit
- Wall effects on terminal falling velocity of spherical particles moving in a Carreau model fluid
- The effect of the physical properties of the liquid phase on the gas-liquid mass transfer coefficient in two- and three-phase agitated systems
- Effectiveness of nitric oxide ozonation
- Modelling of nanocrystalline iron nitriding process — influence of specific surface area
- Effect of CeO2 and Sb2O3 on the phase transformation and optical properties of photostable titanium dioxide
- Carnauba wax microparticles produced by melt dispersion technique
- Complexation studies of 3-substituted β-diketones with selected d- and f-metal ions
- Influence of the solvent donor number on the O/W partition ratio of Cu(II) complexes of 1,2-dialkylimidazoles
- Continuous dialysis of sulphuric acid in the presence of zinc sulphate
- Differences in affinity of arylstilbazolium derivatives to tetraplex structures
- Fast ferritin immunoassay on PDMS microchips
