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Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models
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Durairaj Ashokkumar
Published/Copyright:
May 20, 2008
Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.
Keywords: lipid peroxidation; DPPH radical; superoxide scavenging; total phenolic content; Oxystelma esculentum
Published Online: 2008-5-20
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Keywords for this article
lipid peroxidation;
DPPH radical;
superoxide scavenging;
total phenolic content;
Oxystelma esculentum
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