In vitro study of the interaction between neptunium ions and aposerumtransferrin by absorption spectrophotometry and ultrafiltration: the case of Np(V)
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R. Racine
Abstract
The interaction between Np(V) and human aposerumtransferrin (apoTf) was studied in vitro under physiological conditions (37°C, pH 7.4, [NaCl]=0.15 M, [HEPES]=5×10-2 M) by UV-visible and near-IR absorption spectrophotometry and by ultrafiltration. It was found that Np(V) was bound in small fraction (<12%) to apoTf, and that the role of carbonate and citrate anions in binding is more competitive than synergistic. The complexes NpO2CO3- and NpO2Cit2- tend to form rather than the Np(V)-apoTf complex. Np(V) binding on apoTf appears to be pH-dependent and reversible. Displacement experiments with FeIIINTA showed non-specific binding, suggesting a weak interaction between Np(V) and apoTf different from the reactions involving transferrin-specific sites with other metallic ions, such as Fe(III). The low overall charge, the size and the geometry of the linear di-oxocation NpO2+ could account for the weak interaction between Np(V) and human transferrin.
© 2003 Oldenbourg Wissenschaftsverlag GmbH
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Articles in the same Issue
- Some rules to improve the energy resolution in alpha liquid scintillation with beta rejection
- Confidence intervals for experiments with background and small numbers of events
- Solubility product of Pu(VI) hydrous oxide
- Precipitation of crystalline neptunium dioxide from near-neutral aqueous solution
- Stability of U(VI) solid phases in the U(VI)-Ca2+-SiO2-OH system
- Synergistic extraction of Th(IV) by 2-hydroxy-1-naphthaldehyde thiosemicarbazone and neutral donors
- Production of 55Co via the 54Fe(d,n)-process and excitation functions of 54Fe(d,t)53Fe and 54Fe(d,α)52mMn reactions from threshold up to 13.8 MeV
- Potential in vivo generator for alpha-particle therapy with 212Bi: Presentation of a system to minimize escape of daughter nuclide after decay of 212Pb to 212Bi
- In vitro study of the interaction between neptunium ions and aposerumtransferrin by absorption spectrophotometry and ultrafiltration: the case of Np(V)
