Thymidine kinase enzyme selective imaging radiopharmaceutical: 99mTc(CO)3-Ganciclovir
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Buket Gedik
Abstract
The aim of this study is to radiolabel ganciclovir, known as having selective antiviral properties against thymidine kinase, with technetium tricarbonylcore (99mTc(CO)3+) and to investigate the biological behavior of this complex in vitro and in vivo. Commercially provided Ganciclovir (GCV) was radiolabeled with 99mTc(CO)3+. Initially, optimum radiolabeling conditions were determined by analyzing factors such as temperature, pH and time. Quality control of the radiolabeled compound was performed. The radiolabeling yield was found to be 97%. The 99mTc(CO)3-GCV complex also displayed good in vitro stability during the 24 h period. In vitro cell uptake studies showed that the 99mTc(CO)3-GCV complex is highly uptaken in A-549, PC-3, HeLa cell lines according to the control group 99mTc(I)-tricarbonyl core. The knowledge gained from in vivo and in vitro studies of 99mTc(CO)3-GCV could contribute to the development of a new HSV1-tk gene imaging agent.
© by Oldenbourg Wissenschaftsverlag, München, Germany
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Articles in the same Issue
- Cross section measurements and theoretical calculations of proton induced nuclear reactions on natural tellurium
- Speciation of Am(III)/Eu(III) sorbed on γ-alumina: effect of metal ion concentration
- Correlation of retention of lanthanide and actinide complexes with stability constants and their speciation
- Modified synthesis scheme for N,N´-dimethyl-N,N´-dioctyl-2,(2´-hexyloxyethyl) malonamide (DMDOHEMA) and its comparison with proposed solvents for actinide partitioning
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- Interactions of Tc(IV) with citrate in NaCl media
- Complexation of rhenium with ethylenediaminetetraacetic acid: studies of technetium analogue using Raman spectroscopy
- Thymidine kinase enzyme selective imaging radiopharmaceutical: 99mTc(CO)3-Ganciclovir
- A study of the surface erosion of zircaloy material during laser ablation process by thin layer activation technique
