In vivo212Pb/212Bi generator using indium-DTPA-tagged liposomes
-
A. Le Du
Abstract
Indium-DTPA-tagged liposomes were studied in the present work as carriers of in vivo212Pb/ 212Bi generator to be used in targeted alpha therapy. The liposomal uptake of 212Pb, into preformed liposomes, was investigated using different lipophilic chelate (DCP, 2,3-dimercapto-1-propanol (BAL), sodium acetate, and A23187), as a function of various parameters (temperature, concentrations of lipids, Pb, DTPA, ...) with 212Pb as a tracer. Different formulations of liposomes were tested to evaluate the radiolabeling efficiency. No complexing agent was necessary for the passage of Pb2+ through the membrane. It occurs naturally via a partial permeability of the lipid bilayer, which increases with the temperature. A complexing agent (DTPA) appears necessary to concentrate Pb in the internal compartment of the liposomes. Conditions were found (T=65 ºC, internal DTPA concentration of 0.025 M, pH 7.4, ...) yielding a high and rapid uptake of 212Pb in liposomes. The protocol established provides a novel method for the efficient entrapment of about 2–3 Pb atoms per liposome with a yield of 75% in conditions relevant for nuclear medicine.
© by Oldenbourg Wissenschaftsverlag, Nantes, Germany
Articles in the same Issue
- Tricarbonate complex of hexavalent Am with guanidinium: synthesis and structural characterization of [C(NH2)3]4[AmO2(CO3)3]·2H2O, comparison with [C(NH2)3]4[AnO2(CO3)3] (An=U, Np, Pu)
- Ternary uranium(VI) carbonato humate complex studied by cryo-TRLFS
- Influence of contact time on the extraction of 233uranyl spike and contaminant uranium from Hanford Site sediment
- Thermodynamics of complexation of lanthanides with 2,6-bis(5,6-diethyl-1,2,4-triazin-3-yl) pyridine
- Extraction of caesium(I) from HNO3 medium using room temperature ionic liquid containing calix[4]crown ligands as the selective extractants
- Bioaccumulation of polonium (210Po) and uranium (234U, 238U) in plants around phosphogypsum waste heap in Wiślinka (northern Poland)
- Development of a micro electrochemical cell for in-situ deposition of 63Ni for use in electron capture detector (ECD) in gas chromatography
- In vivo212Pb/212Bi generator using indium-DTPA-tagged liposomes
- Determination of trace impurities of chlorine in zirconium-alloy matrices using neutron activation technique
Articles in the same Issue
- Tricarbonate complex of hexavalent Am with guanidinium: synthesis and structural characterization of [C(NH2)3]4[AmO2(CO3)3]·2H2O, comparison with [C(NH2)3]4[AnO2(CO3)3] (An=U, Np, Pu)
- Ternary uranium(VI) carbonato humate complex studied by cryo-TRLFS
- Influence of contact time on the extraction of 233uranyl spike and contaminant uranium from Hanford Site sediment
- Thermodynamics of complexation of lanthanides with 2,6-bis(5,6-diethyl-1,2,4-triazin-3-yl) pyridine
- Extraction of caesium(I) from HNO3 medium using room temperature ionic liquid containing calix[4]crown ligands as the selective extractants
- Bioaccumulation of polonium (210Po) and uranium (234U, 238U) in plants around phosphogypsum waste heap in Wiślinka (northern Poland)
- Development of a micro electrochemical cell for in-situ deposition of 63Ni for use in electron capture detector (ECD) in gas chromatography
- In vivo212Pb/212Bi generator using indium-DTPA-tagged liposomes
- Determination of trace impurities of chlorine in zirconium-alloy matrices using neutron activation technique
