Development of [201Tl](III)-DTPA-human polyclonal antibody complex for inflammation detection
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A. R. Jalilian
Thallium-201 (T1/2=3.04 d) in Tl+ form was converted to Tl3+ cation in presence of O3 in 6 M HCl controlled by RTLC/gel electrophoresis methods and used in the labeling of human polyclonal antibody (HIgG) after conjugation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 mL of a HIgG pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 °C with continuous mild stirring for 30 min. The final isotonic [201Tl](III)-DTPA-HIgG complex was checked by radio-TLC using several solvent systems to ensure the formation of only one species followed by filtration through a 0.22 μ filter (specific activity= 33.7 TBq/mM, radiochemical purity >95%). Preliminary bio-distribution studies in normal and inflammation-bearing rats were performed. The target/skin and target/blood ratios were 4 and 6 after 28 h respectively, showing the selectivity of the radiopharmaceutical for the inflammatory lesions.
© Oldenbourg Wissenschaftsverlag
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Articles in the same Issue
- Sulphate complexation of protactinium(V) at 25 °C
- Separation of actinides from fission products by extraction chromatography prior to X-ray fluorescence measurement for analytical control of a PUREX test
- Separation of actinides by extraction chromatography prior to X-ray fluorescence measurement for analytical control of a PUREX test
- The behaviour of organic solvents containing C5-BTBP and CyMe4-BTBP at low irradiation doses
- Study of RuO4 decomposition in dry and moist air
- Standardized high current solid tellurium-124 target for cyclotron production of the radionuclides iodine-123,124
- Lead (Pb) column for concentration of 99mTc-pertechnetate
- Development of [201Tl](III)-DTPA-human polyclonal antibody complex for inflammation detection