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Natural deep eutectic solvents: composition, preparation, and applications in food, pharmaceutical, and biomedical sciences

  • Sangeeta Yadav ORCID logo , Alka Sharma ORCID logo EMAIL logo , Akash Kumar ORCID logo EMAIL logo and Tanbeer Kaur ORCID logo
Published/Copyright: November 28, 2025

Abstract

Natural deep eutectic solvents (NADES) have emerged as a green, sustainable alternative to conventional organic solvents due to their biocompatibility and non-toxicity. This review highlights the composition and structural diversity of NADES. The various techniques of NADES preparation are discussed, along with the critical factors that influence their stability and functionality. A wide range of NADES applications was discussed, such as their role in the extraction of bioactive compounds, food analysis, biocatalysis, and cryoprotection. Additionally, the review examines the potential applications of NADES in pharmaceutical and biomedical sciences, including their role in drug delivery, burn injury management, and wound treatment. Despite these potentials, there are certain limitations of NADES, such as high viscosity and difficulty in product recovery. Thus, there is a need for a standardized methodology to understand NADES interactions at the molecular level and to develop cost-effective commercial processes for product recovery.


Corresponding authors: Alka Sharma, Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, India, E-mail: ; and Akash Kumar, MMICT & BM (HM), Maharishi Markandeshwar (Deemed to be University), Mullana, 133207, India, E-mail:

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: Conceptualization: AS and TK; supervision: AS and TK; visualization: SY and TK; writing – original draft: SY and AK; writing – review and editing: SY, AK and AS.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The author states no conflict of interest.

  6. Research funding: Not applicable.

  7. Data availability: Not applicable.

References

1. Swebocki, T, Barras, A, Abderrahmani, A, Haddadi, K, Boukherroub, R. Deep eutectic solvents comprising organic acids and their application in (Bio)medicine. Int J Mol Sci 2023;24:8492. https://doi.org/10.3390/ijms24108492.Search in Google Scholar PubMed PubMed Central

2. Marset, X, Guillena, G. Deep eutectic solvents as à-la-carte medium for transition-metal-catalyzed organic processes. Molecules 2022;27:8445. https://doi.org/10.3390/molecules27238445.Search in Google Scholar PubMed PubMed Central

3. Nian, B, Li, X. Can deep eutectic solvents be the best alternatives to ionic liquids and organic solvents: a perspective in enzyme catalytic reactions. Int J Biol Macromol 2022;217:255–69. https://doi.org/10.1016/j.ijbiomac.2022.07.044.Search in Google Scholar PubMed

4. Hansen, BB, Spittle, S, Chen, B, Poe, D, Zhang, Y, Klein, JM, et al.. Deep eutectic solvents: a review of fundamentals and applications. Chem Rev 2021;121:1232–85. https://doi.org/10.1021/acs.chemrev.0c00385.Search in Google Scholar PubMed

5. Shokri, S, Ebrahimi, N, Sadeghi, R. Theoretical and experimental study of molecular interactions between constituents of deep eutectic solvents. Fluid Phase Equilib 2024;583:114121. https://doi.org/10.1016/j.fluid.2024.114121.Search in Google Scholar

6. Wysokowski, M, Luu, RK, Arevalo, S, Khare, E, Stachowiak, W, Niemczak, M, et al.. Untapped potential of deep eutectic solvents for the synthesis of bioinspired inorganic-organic materials. Chem Mater 2023;35:7878–903. https://doi.org/10.1021/acs.chemmater.3c00847.Search in Google Scholar PubMed PubMed Central

7. Usmani, Z, Sharma, M, Tripathi, M, Lukk, T, Karpichev, Y, Gathergood, N, et al.. Biobased natural deep eutectic system as versatile solvents: structure, interaction and advanced applications. Sci Total Environ 2023;881:163002. https://doi.org/10.1016/j.scitotenv.2023.163002.Search in Google Scholar PubMed

8. Indoria, S, Kaur, G, Singh, V. Unveiling the potential of deep eutectic solvents: catalysis through solvent innovation. Chem Afr 2025:1–13. https://doi.org/10.1007/s42250-025-01278-0.Search in Google Scholar

9. Bouizgma, K, Rabbah, N, Abbas, Z, Abourriche, A. Unlocking sustainable extraction of natural antioxidants: green solvents, smart technologies, scalability and future directions. Separ Sci Technol 2025. https://doi.org/10.1080/01496395.2025.2452411.Search in Google Scholar

10. Bencresciuto, GF, Carnevale, M, Paris, E, Gallucci, F, Santangelo, E, Migliori, CA. A sustainable alternative for cosmetic applications: NADES extraction of bioactive compounds from hazelnut By-Products. Sustainability 2025;17:1516. https://doi.org/10.3390/su17041516.Search in Google Scholar

11. Balabram, SK, Tessaro, L, Astolfo, MEde A, Sponchiado, PAI, Bogusz Junior, S, Maniglia, BC. Development of NADES–annatto seed extract for enhancing 3D printed food designed for Dysphagia patients. Food 2025;14:1604. https://doi.org/10.3390/foods14091604.Search in Google Scholar PubMed PubMed Central

12. Gallo, M, Makarova, A, Özten, C, Zieniuk, B. Utilizing natural deep eutectic solvents (NADESs) for sustainable phytonutrient recovery: optimization and multi-matrix extraction of bioactive compounds. Appl Sci 2025;15:4843. https://doi.org/10.3390/app15094843.Search in Google Scholar

13. Chevé-Kools, E, Hae Choi, Y, Roullier, C, Ruprich-Robert, G, Grougnet, R, Chapeland-Leclerc, F, Hollmann, F. Natural deep eutectic solvents (NaDES): green solvents for pharmaceutical applications and beyond. Green Chem 2025;27:8360–85.10.1039/D4GC06386DSearch in Google Scholar

14. Gómez-Urios, C, Viñas-Ospino, A, Puchades-Colera, P, López-Malo, D, Frígola, A, Esteve, MJ, et al.. Sustainable development and storage stability of Orange By-Products extract using natural deep eutectic solvents. Foods 2022;11. https://doi.org/10.3390/foods11162457.Search in Google Scholar PubMed PubMed Central

15. Zannou, O, Koca, I. Greener extraction of anthocyanins and antioxidant activity from blackberry (Rubus spp) using natural deep eutectic solvents. LWT – Lebensm-Wiss & Technol 2022;158. https://doi.org/10.1016/j.lwt.2022.113184.Search in Google Scholar

16. Kurtulbaş, E, Pekel, AG, Bilgin, M, Makris, DP, Şahin, S. Citric acid-based deep eutectic solvent for the anthocyanin recovery from Hibiscus sabdariffa through microwave-assisted extraction. Biomass Convers Biorefinery 2022;12:351–60. https://doi.org/10.1007/s13399-020-00606-3.Search in Google Scholar

17. Dwamena, AK. Recent advances in hydrophobic deep eutectic solvents for extraction. Separations 2019;6. https://doi.org/10.3390/separations6010009.Search in Google Scholar

18. Plastiras, OE, Samanidou, V. Applications of deep eutectic solvents in sample preparation and extraction of organic molecules. Molecules 2022;27. https://doi.org/10.3390/molecules27227699.Search in Google Scholar PubMed PubMed Central

19. Wu, K, Ren, J, Wang, Q, Nuerjiang, M, Xia, X, Bian, C. Research progress on the preparation and action mechanism of natural deep eutectic solvents and their application in food. Foods 2022;11. https://doi.org/10.3390/foods11213528.Search in Google Scholar PubMed PubMed Central

20. Liu, Y, Friesen, JB, McAlpine, JB, Lankin, DC, Chen, SN, Pauli, GF. Natural deep eutectic solvents: properties, applications, and perspectives. J Nat Prod 2018;81:679–90. https://doi.org/10.1021/acs.jnatprod.7b00945.Search in Google Scholar PubMed PubMed Central

21. Ribeiro, BD, Florindo, C, Iff, LC, Coelho, MAZ, Marrucho, IM. Menthol-based eutectic mixtures: hydrophobic low viscosity solvents. ACS Sustainable Chem Eng 2015;3:2469–77. https://doi.org/10.1021/acssuschemeng.5b00532.Search in Google Scholar

22. Vanda, H, Dai, Y, Wilson, EG, Verpoorte, R, Choi, YH. Green solvents from ionic liquids and deep eutectic solvents to natural deep eutectic solvents. C R Chim 2018;21:628–38. https://doi.org/10.1016/j.crci.2018.04.002.Search in Google Scholar

23. Yang, Z. Natural deep eutectic solvents and their applications in biotechnology. In: Advances in Biochemical Engineering/Biotechnology. Springer Science and Business Media Deutschland GmbH; 2019:31–59 pp.10.1007/10_2018_67Search in Google Scholar PubMed

24. Mišan, A, Nađpal, J, Stupar, A, Pojić, M, Mandić, A, Verpoorte, R, et al.. The perspectives of natural deep eutectic solvents in agri-food sector. Crit Rev Food Sci Nutr 2020;60:2564–92. https://doi.org/10.1080/10408398.2019.1650717.Search in Google Scholar PubMed

25. Hikmawanti, NPE, Ramadon, D, Jantan, I, Mun’im, A. Natural deep eutectic solvents (Nades): phytochemical extraction performance enhancer for pharmaceutical and nutraceutical product development. Plants 2021;10. https://doi.org/10.3390/plants10102091.Search in Google Scholar PubMed PubMed Central

26. Jauregi, P, Esnal-Yeregi, L, Labidi, J. Natural deep eutectic solvents (NADES) for the extraction of bioactives: emerging opportunities in biorefinery applications. PeerJ Anal Chem 2024;6:e32. https://doi.org/10.7717/peerj-achem.32.Search in Google Scholar

27. Schuh, L, Reginato, M, Florêncio, I, Falcao, L, Boron, L, Gris, EF, et al.. From nature to innovation: the uncharted potential of natural deep eutectic solvents. Molecules 2023;28. https://doi.org/10.3390/molecules28227653.Search in Google Scholar PubMed PubMed Central

28. Duan, L, Dou, LL, Guo, L, Li, P, Liu, EH. Comprehensive evaluation of deep eutectic solvents in extraction of bioactive natural products. ACS Sustainable Chem Eng 2016;4:2405–11. https://doi.org/10.1021/acssuschemeng.6b00091.Search in Google Scholar

29. Freitas, DS. Natural deep eutectic solvents-NADES: green solvents for extraction and enzymatic polymerization processes [Ph.D. thesis] Braga, Portugal: Universidade do Minho; 2024. Available from: https://hdl.handle.net/1822/93532.Search in Google Scholar

30. Lazović, M, Cvijetić, I, Jankov, M, Milojković-Opsenica, D, Trifković, J, Ristivojević, P. COSMO-RS in prescreening of natural eutectic solvents for phenolic extraction from Teucrium chamaedrys. J Mol Liq 2023;387:122649. https://doi.org/10.1016/j.molliq.2023.122649.Search in Google Scholar

31. Wojeicchowski, JP, Ferreira, AM, Abranches, DO, Mafra, MR, Coutinho, JAP. Using COSMO-RS in the design of deep eutectic solvents for the extraction of antioxidants from rosemary. ACS Sustainable Chem Eng 2020;8:12132–41. https://doi.org/10.1021/acssuschemeng.0c03553.Search in Google Scholar

32. Jeliński, T, Cysewski, P. Application of a computational model of natural deep eutectic solvents utilizing the COSMO-RS approach for screening of solvents with high solubility of rutin. J Mol Model 2018;24. https://doi.org/10.1007/s00894-018-3700-1.Search in Google Scholar PubMed PubMed Central

33. Van Osch, DJGP, Dietz, CHJT, Van Spronsen, J, Kroon, MC, Gallucci, F, van Sint Annaland, M, et al.. A search for natural hydrophobic deep eutectic solvents based on natural components. ACS Sustainable Chem Eng 2019;7:2933–42. https://doi.org/10.1021/acssuschemeng.8b03520.Search in Google Scholar

34. Shafie, MH, Yusof, R, Gan, CY. Synthesis of citric acid monohydrate-choline chloride based deep eutectic solvents (DES) and characterization of their physicochemical properties. J Mol Liq 2019;288. https://doi.org/10.1016/j.molliq.2019.111081.Search in Google Scholar

35. Viñas-Ospino, A, Panić, M, Bagović, M, Radošević, K, Esteve, M, Radojčić Redovniković, I. Green approach to extract bioactive compounds from orange peel employing hydrophilic and hydrophobic deep eutectic solvents. Sustain Chem Pharm 2023;31. https://doi.org/10.1016/j.scp.2022.100942.Search in Google Scholar

36. El Achkar, T, Greige-Gerges, H, Fourmentin, S. Basics and properties of deep eutectic solvents: a review. Environ Chem Lett 2021;19:3397–408. https://doi.org/10.1007/s10311-021-01225-8.Search in Google Scholar

37. Fernández, Mde los Á, Espino, M, Gomez, FJV, Silva, MF. Novel approaches mediated by tailor-made green solvents for the extraction of phenolic compounds from agro-food industrial by-products. Food Chem 2018;239:671–8. https://doi.org/10.1016/j.foodchem.2017.06.150.Search in Google Scholar PubMed

38. Crawford, DE, Wright, LA, James, SL, Abbott, AP. Efficient continuous synthesis of high purity deep eutectic solvents by twin screw extrusion. Chem Commun 2016;52:4215–18. https://doi.org/10.1039/c5cc09685e.Search in Google Scholar PubMed

39. Crawford, D, Miskimmin, C, Albadarin, A, Walker, G, James, SL. Organic synthesis by Twin Screw Extrusion (TSE): continuous, scalable and solvent-free. Green Chem 2017;19:1507–18. https://doi.org/10.1039/c6gc03413f.Search in Google Scholar

40. Wolbert, F, Brandenbusch, C, Sadowski, G. Selecting excipients forming therapeutic deep eutectic systems-a mechamistic approach. Mol Pharm 2019;16:3091–9. https://doi.org/10.1021/acs.molpharmaceut.9b00336.Search in Google Scholar PubMed

41. Rubin Pedrazzo, A, Cecone, C, Trotta, F, Zanetti, M. Mechanosynthesis of β-cyclodextrin polymers based on natural deep eutectic solvents. ACS Sustainable Chem Eng 2021;9:14881–9. https://doi.org/10.1021/acssuschemeng.1c04988.Search in Google Scholar

42. Velásquez, P, Bustos, D, Montenegro, G, Giordano, A. Ultrasound-assisted extraction of anthocyanins using natural deep eutectic solvents and their incorporation in edible films. Molecules 2021;26. https://doi.org/10.3390/molecules26040984.Search in Google Scholar PubMed PubMed Central

43. Cannavacciuolo, C, Pagliari, S, Frigerio, J, Giustra, CM, Labra, M, Campone, L. Natural deep eutectic solvents (NADESs) combined with sustainable extraction techniques: a review of the green chemistry approach in food analysis. Foods 2023;12. https://doi.org/10.3390/foods12010056.Search in Google Scholar PubMed PubMed Central

44. Craveiro, R, Aroso, I, Flammia, V, Carvalho, T, Viciosa, M, Dionísio, M, et al.. Properties and thermal behavior of natural deep eutectic solvents. J Mol Liq 2016;215:534–40. https://doi.org/10.1016/j.molliq.2016.01.038.Search in Google Scholar

45. Dai, Y, van Spronsen, J, Witkamp, GJ, Verpoorte, R, Choi, YH. Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta 2013;766:61–8. https://doi.org/10.1016/j.aca.2012.12.019.Search in Google Scholar PubMed

46. Gomez, FJV, Espino, M, Fernández, MA, Silva, MF. A greener approach to prepare natural deep eutectic solvents. Wiley Online Library 2018;3:6122–5.10.1002/slct.201800713Search in Google Scholar

47. Santana, APR, Mora-Vargas, JA, Guimarães, TGS, Amaral, CD, Oliveira, A, Gonzalez, MH. Sustainable synthesis of natural deep eutectic solvents (NADES) by different methods. J Mol Liq 2019;293. https://doi.org/10.1016/j.molliq.2019.111452.Search in Google Scholar

48. Ruesgas-Ramón, M, Figueroa-Espinoza, MC, Durand, E. Application of deep eutectic solvents (DES) for phenolic compounds extraction: overview, challenges, and opportunities. J Agric Food Chem 2017;65:3591–601. https://doi.org/10.1021/acs.jafc.7b01054.Search in Google Scholar PubMed

49. Rente, D, Paiva, A, Duarte, AR. The role of hydrogen bond donor on the extraction of phenolic compounds from natural matrices using deep eutectic systems. Molecules 2021;26. https://doi.org/10.3390/molecules26082336.Search in Google Scholar PubMed PubMed Central

50. Benvenutti, L, Zielinski, AAF, Ferreira, SRS. Which is the best food emerging solvent: IL, DES or NADES? Trends Food Sci Technol 2019;90:133–46. https://doi.org/10.1016/j.tifs.2019.06.003.Search in Google Scholar

51. Emami, S, Shayanfar, A. Deep eutectic solvents for pharmaceutical formulation and drug delivery applications. Pharmaceut Dev Technol 2020;25:779–96. https://doi.org/10.1080/10837450.2020.1735414.Search in Google Scholar PubMed

52. Saini, A, Kumar, A, Panesar, PS, Thakur, A. Potential of deep eutectic solvents in the extraction of value-added compounds from agro‐industrial by-products. Appl Food Res 2022;2. https://doi.org/10.1016/j.afres.2022.100211.Search in Google Scholar

53. Nunes, RJ, Saramago, B, Marrucho, IM. Surface tension of dl-menthol:octanoic acid eutectic mixtures. J Chem Eng Data 2019;64:4915–23. https://doi.org/10.1021/acs.jced.9b00424.Search in Google Scholar

54. Zhang, Q, De Oliveira Vigier, K, Royer, S, Jérôme, F. Deep eutectic solvents: syntheses, properties and applications. Chem Soc Rev 2012;41:7108–46. https://doi.org/10.1039/c2cs35178a.Search in Google Scholar PubMed

55. Ghaedi, H, Ayoub, M, Sufian, S, Shariff, AM, Lal, B. The study on temperature dependence of viscosity and surface tension of several phosphonium-based deep eutectic solvents. J Mol Liq 2017;241:500–10. https://doi.org/10.1016/j.molliq.2017.06.024.Search in Google Scholar

56. Huang, Y, Feng, F, Jiang, J, Qiao, Y, Wu, T, Voglmeir, J, et al.. Green and efficient extraction of rutin from tartary buckwheat hull by using natural deep eutectic solvents. Food Chem 2017;221:1400–5. https://doi.org/10.1016/j.foodchem.2016.11.013.Search in Google Scholar PubMed

57. Leng, KY, Suyin, G. Natural deep eutectic solvent (NADES) as a greener alternative for the extraction of hydrophilic (polar) and lipophilic (non-polar) phytonutrients. Key Eng Mater 2019;797:20–8. https://doi.org/10.4028/www.scientific.net/kem.797.20.Search in Google Scholar

58. Gullón, P, Gullón, B, Romaní, A, Rocchetti, G, Lorenzo, JM. Smart advanced solvents for bioactive compounds recovery from agri-food by-products: a review. Trends Food Sci Technol 2020;101:182–97. https://doi.org/10.1016/j.tifs.2020.05.007.Search in Google Scholar

59. Martins, MAR, Pinho, SP, Coutinho, JAP. Insights into the nature of eutectic and deep eutectic mixtures. J Solut Chem 2019;48:962–82. https://doi.org/10.1007/s10953-018-0793-1.Search in Google Scholar

60. Bosiljkov, T, Dujmić, F, Cvjetko Bubalo, M, Hribar, J, Vidrih, R, Brnčić, M, et al.. Natural deep eutectic solvents and ultrasound-assisted extraction: green approaches for extraction of wine lees anthocyanins. Food Bioprod Process 2017;102:195–203. https://doi.org/10.1016/j.fbp.2016.12.005.Search in Google Scholar

61. Cvjetko, BM, Ćurko, N, Tomašević, M, Kovačević Ganić, K, Radojcic Redovnikovic, I. Green extraction of grape skin phenolics by using deep eutectic solvents. Food Chem 2016;200:159–66. https://doi.org/10.1016/j.foodchem.2016.01.040.Search in Google Scholar PubMed

62. Dai, Y, Rozema, E, Verpoorte, R, Choi, YH. Application of natural deep eutectic solvents to the extraction of anthocyanins from Catharanthus roseus with high extractability and stability replacing conventional organic solvents. J Chromatogr A 2016;1434:50–6. https://doi.org/10.1016/j.chroma.2016.01.037.Search in Google Scholar PubMed

63. Maimulyanti, A, Nurhidayati, I, Mellisani, B, Amelia Rachmawati Putri, F, Puspita, F, Restu Prihadi, A. Development of natural deep eutectic solvent (NADES) based on choline chloride as a green solvent to extract phenolic compound from coffee husk waste. Arab J Chem 2023;16. https://doi.org/10.1016/j.arabjc.2023.104634.Search in Google Scholar

64. Leron, RB, Wong, DSH, Li, MH. Densities of a deep eutectic solvent based on choline chloride and glycerol and its aqueous mixtures at elevated pressures. Fluid Phase Equilib 2012;335:32–8. https://doi.org/10.1016/j.fluid.2012.08.016.Search in Google Scholar

65. Negi, T, Kumar, A, Sharma, SK, Rawat, N, Saini, D, Sirohi, R, et al.. Deep eutectic solvents: preparation, properties, and food applications. Heliyon 2024;10. https://doi.org/10.1016/j.heliyon.2024.e28784.Search in Google Scholar PubMed PubMed Central

66. Hayyan, M, Mbous, YP, Looi, CY, Wong, WF, Hayyan, A, Salleh, Z, et al.. Natural deep eutectic solvents: cytotoxic profile. SpringerPlus 2016;5. https://doi.org/10.1186/s40064-016-2575-9.Search in Google Scholar PubMed PubMed Central

67. Zhang, M, Zhang, X, Liu, Y, Wu, K, Zhu, Y, Lu, H, et al.. Insights into the relationships between physicochemical properties, solvent performance, and applications of deep eutectic solvents. Environ Sci Pollut Control Ser 2021;28:35537–63. https://doi.org/10.1007/s11356-021-14485-2.Search in Google Scholar PubMed

68. García, G, Aparicio, S, Ullah, R, Atilhan, M. Deep eutectic solvents: physicochemical properties and gas separation applications. Energy Fuels 2015;29:2616–44. https://doi.org/10.1021/ef5028873.Search in Google Scholar

69. Ibrahim, RK, Hayyan, M, AlSaadi, MA, Ibrahim, S, Hayyan, A, Hashim, MA. Physical properties of ethylene glycol-based deep eutectic solvents. J Mol Liq 2019;276:794–800. https://doi.org/10.1016/j.molliq.2018.12.032.Search in Google Scholar

70. Kawase, K, Abe, J, Tenjimbayashi, M, Kobayashi, Y, Takahashi, K, Shiratori, S. Novel deep-eutectic-solvent-infused carbon nanofiber networks as high power density green battery cathodes. ACS Appl Mater Interfaces 2018;10:15742–50. https://doi.org/10.1021/acsami.8b03099.Search in Google Scholar PubMed

71. Mjalli, FS, Mousa, H. Viscosity of aqueous ionic liquids analogues as a function of water content and temperature. Chin J Chem Eng 2017;25:1877–83. https://doi.org/10.1016/j.cjche.2017.09.008.Search in Google Scholar

72. Dai, Y, Witkamp, GJ, Verpoorte, R, Choi, YH. Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. Food Chem 2015;187:14–19. https://doi.org/10.1016/j.foodchem.2015.03.123.Search in Google Scholar PubMed

73. Li, D. Natural deep eutectic solvents in phytonutrient extraction and other applications. Front Plant Sci 2022;13. https://doi.org/10.3389/fpls.2022.1004332.Search in Google Scholar PubMed PubMed Central

74. Santos-Martín, M, Cubero-Cardoso, J, González-Domínguez, R, Cortés-Triviño, E, Sayago, A, Urbano, J, et al.. Ultrasound-assisted extraction of phenolic compounds from blueberry leaves using natural deep eutectic solvents (NADES) for the valorization of agrifood wastes. Biomass Bioenergy 2023;175. https://doi.org/10.1016/j.biombioe.2023.106882.Search in Google Scholar

75. Silva, DTda, Pauletto, R, Cavalheiro, Sda S, Bochi, VC, Rodrigues, E, Weber, J, et al.. Natural deep eutectic solvents as a biocompatible tool for the extraction of blueberry anthocyanins. J Food Compos Anal 2020;89. https://doi.org/10.1016/j.jfca.2020.103470.Search in Google Scholar

76. Rachmaniah, O, Muhsin, MR, Putra, AW, Rachimoellah, M. Purification of curcuminoids from natural deep eutectic solvents (Nades) matrices using chromatography-based separation methods. Indones J Chem 2021;21:806–15. https://doi.org/10.22146/ijc.58935.Search in Google Scholar

77. Osamede Airouyuwa, J, Sivapragasam, N, Ali Redha, A, Maqsood, S. Sustainable green extraction of anthocyanins and carotenoids using deep eutectic solvents (DES): a review of recent developments. Food Chem 2024;448. https://doi.org/10.1016/j.foodchem.2024.139061.Search in Google Scholar PubMed

78. Chen, Y, Zhang, W, Zhao, T, Li, F, Zhang, M, Li, J, et al.. Adsorption properties of macroporous adsorbent resins for separation of anthocyanins from mulberry. Food Chem 2016;194:712–22. https://doi.org/10.1016/j.foodchem.2015.08.084.Search in Google Scholar PubMed

79. Che Zain, MS, Yeoh, JX, Lee, SY, Afzan, A, Shaari, K. Integration of choline chloride-based natural deep eutectic solvents and macroporous resin for green production of enriched oil palm flavonoids as natural wound healing agents. Antioxidants 2021;10. https://doi.org/10.3390/antiox10111802.Search in Google Scholar PubMed PubMed Central

80. Chen, J, Jiang, X, Yang, G, Bi, Y, Liu, W. Green and efficient extraction of resveratrol from peanut roots using deep eutectic solvents. J Chem 2018;2018. https://doi.org/10.1155/2018/4091930.Search in Google Scholar

81. Lanjekar, KJ, Rathod, VK. Recovery and separation of glycyrrhizic acid from Natural Deep Eutectic Solvent (NADES) extract by macroporous resin: adsorption kinetics and isotherm studies. Prep Biochem Biotechnol 2024;54:39–48. https://doi.org/10.1080/10826068.2023.2204485.Search in Google Scholar PubMed

82. Xu, L, Liaqat, F, Khazi, MI, Sun, J, Zhu, D. Natural deep eutectic solvents-based green extraction of vanillin: optimization, purification, and bioactivity assessment. Front Nutr 2023;10. https://doi.org/10.3389/fnut.2023.1279552.Search in Google Scholar PubMed PubMed Central

83. Panić, M, Gunjević, V, Cravotto, G, Radojčić Redovniković, I. Enabling technologies for the extraction of grape-pomace anthocyanins using natural deep eutectic solvents in up-to-half-litre batches extraction of grape-pomace anthocyanins using NADES. Food Chem 2019;300. https://doi.org/10.1016/j.foodchem.2019.125185.Search in Google Scholar PubMed

84. Zhang, S, Lin, S, Zhang, J, Liu, W. Ultrasound-assisted natural deep eutectic solvent extraction of anthocyanin from Vitis davidii Foex. pomace: optimization, identification, antioxidant activity and stability. Heliyon 2024;10. https://doi.org/10.1016/j.heliyon.2024.e33066.Search in Google Scholar PubMed PubMed Central

85. Stupar, A, Šeregelj, V, Ribeiro, BD, Pezo, L, Cvetanović, A, Mišan, A, et al.. Recovery of β-carotene from pumpkin using switchable natural deep eutectic solvents. Ultrason Sonochem 2021;76. https://doi.org/10.1016/j.ultsonch.2021.105638.Search in Google Scholar PubMed PubMed Central

86. Doldolova, K, Bener, M, Lalikoğlu, M, Aşçı, YS, Arat, R, Apak, R. Optimization and modeling of microwave-assisted extraction of curcumin and antioxidant compounds from turmeric by using natural deep eutectic solvents. Food Chem 2021;353. https://doi.org/10.1016/j.foodchem.2021.129337.Search in Google Scholar PubMed

87. Xu, M, Ran, L, Chen, N, Fan, X, Ren, D, Yi, L. Polarity-dependent extraction of flavonoids from citrus peel waste using a tailor-made deep eutectic solvent. Food Chem 2019;297. https://doi.org/10.1016/j.foodchem.2019.124970.Search in Google Scholar PubMed

88. Zhang, X, Wang, S, Wu, Q, Battino, M, Giampieri, F, Bai, W, et al.. Recovering high value-added anthocyanins from blueberry pomace with ultrasound-assisted extraction. Food Chem X 2022;16. https://doi.org/10.1016/j.fochx.2022.100476.Search in Google Scholar PubMed PubMed Central

89. Xie, J, Wang, H, Ren, T, Zheng, Q, Chen, M. Optimization of the preparation of high purity ellagic acid from blueberry pomace through an antisolvent precipitation method. Sustain Chem Pharm 2024;42:101776. https://doi.org/10.1016/j.scp.2024.101776.Search in Google Scholar

90. Isci, A, Kaltschmitt, M. Recovery and recycling of deep eutectic solvents in biomass conversions: a review. Biomass Convers Biorefinery 2022;12:197–226. https://doi.org/10.1007/s13399-021-01860-9.Search in Google Scholar

91. Zhang, Y, Bian, S, Hu, J, Liu, G, Peng, S, Chen, H, et al.. Natural deep eutectic solvent-based microwave-assisted extraction of total flavonoid compounds from spent sweet potato (Ipomoea batatas L.) leaves: optimization and antioxidant and bacteriostatic activity. Molecules 2022;27:5985. https://doi.org/10.3390/molecules27185985.Search in Google Scholar PubMed PubMed Central

92. Airouyuwa, JO, Mostafa, H, Riaz, A, Stathopoulos, C, Maqsood, S. Natural deep eutectic solvents and microwave-assisted green extraction for efficient recovery of bioactive compounds from by-products of date fruit (Phoenix dactylifera L.) processing: modeling, optimization, and phenolic characterization. Food Bioprocess Technol 2023;16:824–43. https://doi.org/10.1007/s11947-022-02960-8.Search in Google Scholar

93. Cañadas, R, Sáenz de Miera, B, Méndez, P, González, EJ, González-Miquel, M. Enhanced recovery of natural antioxidants from grape waste using natural eutectic solvents-based microwave-assisted extraction. Molecules 2023;28:1153. https://doi.org/10.3390/molecules28031153.Search in Google Scholar PubMed PubMed Central

94. Kim, H, Jung, YS, Song, NE, Yoo, M, Seo, DH, Kim, HS, et al.. Ultrasound-assisted extraction of major anthocyanins in Korean black raspberries (Rubus coreanus Miquel) using natural deep eutectic solvents. LWT – Lebensm-Wiss & Technol 2024;199:116121. https://doi.org/10.1016/j.lwt.2024.116121.Search in Google Scholar

95. Jovanović, MS, Krgović, N, Živković, J, Stević, T, Zdunić, G, Bigović, D, et al.. Ultrasound-assisted natural deep eutectic solvents extraction of bilberry anthocyanins: optimization, bioactivities, and storage stability. Plants 2022;11. https://doi.org/10.3390/plants11202680.Search in Google Scholar PubMed PubMed Central

96. Alchera, F, Ginepro, M, Giacalone, G. Microwave-assisted extraction (MAE) of bioactive compounds from blueberry by-products using a sugar-based NADES: a novelty in green chemistry. LWT – Lebensm-Wiss & Technol 2024;192:115642. https://doi.org/10.1016/j.lwt.2023.115642.Search in Google Scholar

97. Thakur, R, Gupta, V, Dhar, P, Deka, SC, Das, AB. Ultrasound-assisted extraction of anthocyanin from black rice bran using natural deep eutectic solvents: optimization, diffusivity, and stability. J Food Process Preserv 2022;46. https://doi.org/10.1111/jfpp.16309.Search in Google Scholar

98. Grillo, G, Tabasso, S, Capaldi, G, Radosevic, K, Radojčić-Redovniković, I, Gunjević, V, et al.. Food-waste valorisation: synergistic effects of enabling technologies and eutectic solvents on the recovery of bioactives from violet potato peels. Foods 2023;12. https://doi.org/10.3390/foods12112214.Search in Google Scholar PubMed PubMed Central

99. Stramarkou, M, Oikonomopoulou, V, Panagiotopoulou, M, Papadaki, S, Krokida, M. Sustainable valorisation of peach and apricot waste using green extraction technique with conventional and deep eutectic solvents. Resources 2023;12:72. https://doi.org/10.3390/resources12060072.Search in Google Scholar

100. Viñas-Ospino, A, Panić, M, Radojčić- Redovniković, I, Blesa, J, Esteve, MJ. Using novel hydrophobic deep eutectic solvents to improve a sustainable carotenoid extraction from orange peels. Food Biosci 2023;53. https://doi.org/10.1016/j.fbio.2023.102570.Search in Google Scholar

101. Benvenutti, L, Zielinski, AAF, Ferreira, SRS. Pressurized aqueous solutions of deep eutectic solvent (DES): a green emergent extraction of anthocyanins from a Brazilian berry processing by-product. Food Chem X 2022;13. https://doi.org/10.1016/j.fochx.2022.100236.Search in Google Scholar PubMed PubMed Central

102. Li, H, Liu, Y, Guo, S, Shi, M, Qin, S, Zeng, C. Extraction of ursolic acid from apple peel with hydrophobic deep eutectic solvents: comparison between response surface methodology and artificial neural networks. Foods 2023;12. https://doi.org/10.3390/foods12020310.Search in Google Scholar PubMed PubMed Central

103. Lazzarini, C, Casadei, E, Valli, E, Tura, M, Ragni, L, Bendini, A, et al.. Sustainable drying and green deep eutectic extraction of carotenoids from tomato pomace. Foods 2022;11. https://doi.org/10.3390/foods11030405.Search in Google Scholar PubMed PubMed Central

104. Ali, MC, Chen, J, Zhang, H, Li, Z, Zhao, L, Qiu, H. Effective extraction of flavonoids from Lycium barbarum L. fruits by deep eutectic solvents-based ultrasound-assisted extraction. Talanta 2019;203:16–22. https://doi.org/10.1016/j.talanta.2019.05.012.Search in Google Scholar PubMed

105. Gómez-Urios, C, Viñas-Ospino, A, Puchades-Colera, P, Blesa, J, López-Malo, D, Frígola, A, et al.. Choline chloride-based natural deep eutectic solvents for the extraction and stability of phenolic compounds, ascorbic acid, and antioxidant capacity from citrus sinensis peel. LWT – Lebensm-Wiss & Technol 2023;177. https://doi.org/10.1016/j.lwt.2023.114595.Search in Google Scholar

106. Aslan Türker, D, Doğan, M. Application of deep eutectic solvents as a green and biodegradable media for extraction of anthocyanin from black carrots. LWT – Lebensm-Wiss & Technol 2021;138. https://doi.org/10.1016/j.lwt.2020.110775.Search in Google Scholar

107. Sportiello, L, Marchesi, E, Tolve, R, Favati, F. Green extraction of carotenoids from pumpkin By-Products using natural hydrophobic deep eutectic solvents: preliminary insights. Molecules 2025;30. https://doi.org/10.3390/molecules30030548.Search in Google Scholar PubMed PubMed Central

108. Sulejmanović, M, Panić, M, Redovniković, IR, Milić, N, Drljača, J, Damjanović, A, et al.. Sustainable isolation of ginger (Zingiber officinale) herbal dust bioactive compounds with favorable toxicological profile employing natural deep eutectic solvents (NADES). Food Chem 2025;464:141545. https://doi.org/10.1016/j.foodchem.2024.141545.Search in Google Scholar PubMed

109. Gkioni, MD, Andriopoulos, V, Koutra, E, Hatziantoniou, S, Kornaros, M, Lamari, FN. Ultrasound-assisted extraction of Nannochloropsis oculata with ethanol and betaine: 1,2-propanediol eutectic solvent for antioxidant pigment-rich extracts retaining nutritious the residual biomass. Antioxidants 2022;11:1103. https://doi.org/10.3390/antiox11061103.Search in Google Scholar PubMed PubMed Central

110. Huang, L, Zuo, S, Gao, X, Li, Z, Wang, S, Chen, B, et al.. Extraction and functional properties of pigment from pumpkin peels by a novel green deep eutectic alcohol two-phase system. Sustain Chem Pharm 2023;33:101067. https://doi.org/10.1016/j.scp.2023.101067.Search in Google Scholar

111. Gonzalo, Gde. Biocatalyzed sulfoxidation in presence of deep eutectic solvents. Sustain Chem 2020;1:290–7 https://doi.org/10.3390/suschem1030019.Search in Google Scholar

112. Panić, M, Radović, M, Maros, I, Jurinjak Tušek, A, Cvjetko Bubalo, M, Radojčić Redovniković, I. Development of environmentally friendly lipase-catalysed kinetic resolution of (R,S)-1-phenylethyl acetate using aqueous natural deep eutectic solvents. Process Biochem 2021;102:1–9. https://doi.org/10.1016/j.procbio.2020.12.001.Search in Google Scholar

113. Bi, S, Liu, H, Lin, H, Wang, P. Integration of natural deep-eutectic solvent and surfactant for efficient synthesis of chiral aromatic alcohol mediated by cyberlindnera saturnus whole cells. Biochem Eng J 2021;172:108053. https://doi.org/10.1016/j.bej.2021.108053.Search in Google Scholar

114. Freitas, DS, Rocha, D, Noro, J, Castro, TG, Cavaco-Paulo, A, Silva, C. Eutectic mixtures as green solvents for laccase-catalyzed reactions. ACS Sustainable Chem Eng 2023;11:16594–607. https://doi.org/10.1021/acssuschemeng.3c04944.Search in Google Scholar

115. Jin, S, Ren, Y, Peng, C, Cheng, Y, Liu, W, Fu, Y, et al.. A sustainable and integrated microbial biocatalysis of resveratrol from polygonum cuspidatum siebold & Zucc using cellulose-based immobilised Aspergillus niger with deep eutectic solvent-assisted microreactors. Appl Biochem Biotechnol 2024;197:1726–42. https://doi.org/10.1007/s12010-024-05118-8.Search in Google Scholar PubMed

116. Li, H, Wang, Q, Li, W, Xia, X. Cryoprotective effect of NADES on frozen-thawed mirror carp surimi in terms of oxidative denaturation, structural properties, and thermal stability of myofibrillar proteins. Foods 2023;12:3530, https://doi.org/10.3390/foods12193530.Search in Google Scholar PubMed PubMed Central

117. Jesus, AR, Duarte, ARC, Paiva, A. Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells. Sci Rep 2022;12:1–9. https://doi.org/10.1038/s41598-022-12365-4.Search in Google Scholar PubMed PubMed Central

118. Tian, Y, Sun, DW, Xu, L, Fan, TH, Zhang, ST, Zhu, Z. Bioinspired cryoprotectants enabled by binary natural deep eutectic solvents for sustainable and green cryopreservation. ACS Sustainable Chem Eng 2022;10:7677–91. https://doi.org/10.1021/acssuschemeng.2c01578.Search in Google Scholar

119. Hornberger, K, Li, R, Duarte, ARC, Hubel, A. Natural deep eutectic systems for nature-inspired cryopreservation of cells. AIChE J 2021;67:e17085. https://doi.org/10.1002/aic.17085.Search in Google Scholar

120. Craveiro, R, Castro, VIB, Viciosa, MT, Dionísio, M, Reis, R, Duarte, ARC, et al.. Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation. J Mol Liq 2021;329:115533. https://doi.org/10.1016/j.molliq.2021.115533.Search in Google Scholar

121. Delgado-Rangel, LH, Huerta-Saquero, A, Eufracio-García, N, Meza-Villezcas, A, Mota-Morales, JD, González-Campos, JB. Deep eutectic solvent-assisted phase separation in chitosan solutions for the production of 3D monoliths and films with tailored porosities. Int J Biol Macromol 2020;164:4084–94. https://doi.org/10.1016/j.ijbiomac.2020.08.254.Search in Google Scholar PubMed

122. Pontillo, ARN, Koutsoukos, S, Welton, T, Detsi, A. Investigation of the influence of natural deep eutectic solvents (NaDES) in the properties of chitosan-stabilised films. Mater Adv 2021;2:3954–64. https://doi.org/10.1039/d0ma01008a.Search in Google Scholar

123. Li, W, Zhao, X, Huang, T, Ren, Y, Gong, W, Guo, Y, et al.. Preparation of sodium hyaluronate/dopamine/AgNPs hydrogel based on the natural eutetic solvent as an antibaterial wound dressing. Int J Biol Macromol 2021;191:60–70. https://doi.org/10.1016/j.ijbiomac.2021.09.056.Search in Google Scholar PubMed

124. Filip, D, Macocinschi, D, Balan-Porcarasu, M, Varganici, CD, Dumitriu, RP, Peptanariu, D, et al.. Biocompatible self-assembled hydrogen-bonded gels based on natural deep eutectic solvents and hydroxypropyl cellulose with strong antimicrobial activity. Gels 2022;8:666. https://doi.org/10.3390/gels8100666.Search in Google Scholar PubMed PubMed Central

125. Cerdá-Bernad, D, Pitterou, I, Tzani, A, Detsi, A, Frutos, MJ. Novel chitosan/alginate hydrogels as carriers of phenolic-enriched extracts from saffron floral by-products using natural deep eutectic solvents as green extraction media. Curr Res Food Sci 2023;6:100469. https://doi.org/10.1016/j.crfs.2023.100469.Search in Google Scholar PubMed PubMed Central

126. Wang, Y, Zhang, Y, Lin, Z, Huang, T, Li, W, Gong, W, et al.. A green method of preparing a natural and degradable wound dressing containing aloe vera as an active ingredient. Compos B Eng 2021;222:109047. https://doi.org/10.1016/j.compositesb.2021.109047.Search in Google Scholar

127. Alkhawaja, B, Al-Akayleh, F, Al-Khateeb, A, Nasereddin, J, Ghanim, BY, Bolhuis, A, et al.. Deep eutectic liquids as a topical vehicle for Tadalafil: characterisation and potential wound healing and antimicrobial activity. Molecules 2023;28:2402. https://doi.org/10.3390/molecules28052402.Search in Google Scholar PubMed PubMed Central

128. Mustafa, NR, Spelbos, VS, Witkamp, GJ, Verpoorte, R, Choi, YH. Solubility and stability of some pharmaceuticals in natural deep eutectic solvents-based formulations. Molecules 2021;26:2645. https://doi.org/10.3390/molecules26092645.Search in Google Scholar PubMed PubMed Central

129. Faggian, M, Sut, S, Perissutti, B, Baldan, V, Grabnar, I, Dall’Acqua, S. Natural Deep Eutectic Solvents (NADES) as a tool for bioavailability improvement: pharmacokinetics of rutin dissolved in proline/glycine after oral administration in rats: possible application in nutraceuticals. Molecules 2016;21:1531. https://doi.org/10.3390/molecules21111531.Search in Google Scholar PubMed PubMed Central

130. Silva, JM, Silva, E, Reis, RL. Therapeutic deep eutectic solvents assisted the encapsulation of curcumin in alginate-chitosan hydrogel beads. Sustain Chem Pharm 2021;24:100553. https://doi.org/10.1016/j.scp.2021.100553.Search in Google Scholar

131. Thakur, R, Gupta, V, Ghosh, T, Das, AB. Effect of anthocyanin-natural deep eutectic solvent (lactic acid/fructose) on mechanical, thermal, barrier, and pH-sensitive properties of polyvinyl alcohol based edible films. Food Packag Shelf Life 2022;33:100914. https://doi.org/10.1016/j.fpsl.2022.100914.Search in Google Scholar

132. Athanasiou, PE, Patila, M, Fotiadou, R, Giotopoulou, I, Barkoula, NM, Voutsas, E, et al.. pH-responsive chitosan films enriched with NADES-extracted wine lees anthocyanins for in situ food monitoring. Gels 2025;11:676. https://doi.org/10.3390/gels11090676.Search in Google Scholar PubMed PubMed Central

Received: 2025-07-09
Accepted: 2025-11-16
Published Online: 2025-11-28

© 2025 Walter de Gruyter GmbH, Berlin/Boston

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