Chemical constituents from the Saposhnikovia divaricata and their antiproliferative activity

Yan Sun; Yan Liu; Rui Yang; Si-Yi Wang; Juan Pan; Wei Guan; Hai-Xue Kuang; Yan-Hong Wang; Bing-You Yang

doi:10.1515/znc-2024-0009

Abstract

Nine compounds were isolated and identified from ethanolic extracts of Saposhnikovia divaricata, including one new alkaloid (1), one new pentacyclic triterpenoid (9), and seven known alkaloids (2–8). Structural elucidation of compounds 1 and 9 was established by 1D and 2D NMR spectra referring to the literature, together with high-resolution mass spectrometric analysis. All compounds were evaluated for antiproliferative activity against two cancer cell lines (LN229, A549) in vitro. Compounds (1–9) showed no significant antiproliferative activity.

Keywords: Saposhnikovia divaricata ; alkaloid; pentacyclic triterpenoid; LN229; A549

Corresponding authors: Yan-Hong Wang and Bing-You Yang, Heilongjiang University of Chinese Medicine, Harbin, China, E-mail: wang.yanhong@163.com (Y.-H. Wang), ybywater@163.com (B.-Y. Yang)

Funding source: Science and Technology Innovation Project for College Students

Award Identifier / Grant number: 2023yjscx001

Funding source: Heilongjiang Province “Double First Class” Discipline Collaborative Innovation Achievement Construction Project

Award Identifier / Grant number: LJGXCG2022-096

Funding source: National Natural Science Foundation of China

Award Identifier / Grant number: U22A20370

Funding source: Key Research and Development Plan of Heilongjiang Province

Award Identifier / Grant number: GA21D008

Funding source: Heilongjiang Touyan Innovation Team Program

Award Identifier / Grant number: [2019]5

Funding source: National Natural Science Foundation of China

Award Identifier / Grant number: Unassigned

Research ethics: Not applicable.
Author contributions: Yan Sun performed the whole experiment process as a guide, performed the extraction, isolation, structural elucidation of compounds 1–9. Yan Liu set the overall research objectives. Rui Yang and Si-Yi Wang assayed the antiproliferative activity of compounds 1–9. Juan Pan operated the NMR instrument. Wei Guan measured the HR-ESI-MS spectra. Hai-Xue Kuang supervised the experiments. Yan-Hong Wang and Bing-You Yang managed and coordinated the experiments and provided funding for experiments. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Yan Sun and Yan Liu have contributed equally to this work. The authors state no conflict of interest.
Research funding: This work was financed by National Natural Science Foundation of China (NSFC) (U22A20370), Heilongjiang Touyan Innovation Team Program ([2019]5), Heilongjiang Province “Double First Class” Discipline Collaborative Innovation Achievement Construction Project (LJGXCG2022-096), Key Research and Development Plan of Heilongjiang Province (GA21D008), and Science and Technology Innovation Project for College Students (2023yjscx001).
Data availability: The cell data can be obtained on request from the corresponding author.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/znc-2024-0009).

Received: 2024-01-15

Accepted: 2024-05-10

Published Online: 2024-05-24

Published in Print: 2024-09-25

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Supplementary Material

Chemical constituents from the Saposhnikovia divaricata and their antiproliferative activity

Abstract

References

Supplementary Material

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