Preliminary assessment of an injectable extracellular matrix from decellularized bovine myocardial tissue

Hatice Ercan; Ayşe Eser Elçin; Yaşar Murat Elçin

doi:10.1515/znc-2021-0039

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Article

Preliminary assessment of an injectable extracellular matrix from decellularized bovine myocardial tissue

Hatice Ercan , Ayşe Eser Elçin and Yaşar Murat Elçin

Published/Copyright: May 27, 2021

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From the journal Zeitschrift für Naturforschung C Volume 76 Issue 11-12

Abstract

The goal of this study was to develop an injectable form of decellularized bovine myocardial tissue matrix which could retain high levels of functional ECM molecules, and could gel at physiological temperature. Dissected ventricular tissue was processed by a detergent-based protocol, lyophilized, enzymatically-digested, and neutralized to form the injectable myocardial matrix (IMM). Histochemical analysis, DNA quantification, and agarose gel electrophoresis demonstrated the efficiency of the applied protocol. Chemical, thermal, morphological, and rheological characterization; protein and sulfated glycosaminoglycan (sGAG) content analysis were performed, in vitro biological properties were evaluated. An in vivo histocompatibility and biodegradability study was performed. Histochemistry revealed complete removal of myocardial cells. DNA content analysis revealed a significant decrease (87%) in the nuclear material, while protein and sGAG contents were highly preserved following decellularization. Soluble IMM was capable of turning into gel form at ∼37 °C, indicating selfassembling property. In vitro findings showed the biomaterial was noncytotoxic, nonhemolytic, and supported the attachment and proliferation of mesenchymal stem cells. In vivo study demonstrated IMM was well-tolerated by rats receiving subcutaneous injection. This work demonstrates that the IMM from decellularized bovine myocardial tissue has the potential for use as a feasible regenerative biomaterial in prospective tissue engineering and regenerative medicine studies.

Keywords: bioactive scaffold; decellularized ECM; injectable myocardial matrix; tissue engineering

Corresponding author: Yaşar Murat Elçin, Biochemistry Division, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Tandogan, Ankara 06100, Turkey; and Biovalda Health Technologies, Inc., Ankara, Turkey, E-mail: elcin@ankara.edu.tr

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
Disclosure statement: Y.M.E. is the founder and shareholder of Biovalda Health Technologies, Inc. (Ankara, Turkey). A.E.E. and Y.M.E have patent applications in relation to regenerative biomaterials. The authors declare no competing financial interests in relation to this particular article.

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Received: 2021-02-11

Accepted: 2021-05-08

Published Online: 2021-05-27

Published in Print: 2021-11-25

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Articles in the same Issue

https://doi.org/10.1515/znc-2021-0039

Keywords for this article

bioactive scaffold; decellularized ECM; injectable myocardial matrix; tissue engineering