Folate-conjugated Helix lucorum hemocyanin – preparation, stability, and cytotoxicity
-
Maya Guncheva
,
Krassimira Idakieva
Abstract
This is the first report on the modification of a hemocyanin from Helix lucorum (HlH), a large molluscan respiratory protein, with folic acid (FA). In a two-step synthetic reaction, we prepared samples of HlH conjugated with 20 and 50 FA residues denoted as FA-HlH-1 and FA-HlH-2, respectively. Comparison of the attenuated total reflectance–Fourier transform infrared spectra in the amide I band region showed a structural rearrangement in the HlH that is due to FA conjugation. The changes in the secondary structure were more noticeable for FA-HlH-2. The thermal stability of HlH was not significantly affected by the FA modification, which is consistent with the observed structural similarities with the native protein. Preliminary cytotoxicity assays showed that FA-HlH-1 and FA-HlH-2 stimulate fibroblast proliferation when applied in concentrations of 50 and 100 μg/well. A negligible reduction of fibroblast growth was observed only for FA-HlH-1 and FA-HlH-2, exposed to 200 μg/well for 48 h. We found that FA-HlH-2 exhibits a low to moderate cytotoxic effect on two breast cancer cell lines, which express folate receptors, a hormone-dependent (MCF-7) and a hormone-independent (MDA-MB-231). FA-HlH-2 protects nontransformed cells and affects only neoplastic cells, which could be an advantage, and the protein could have potential in combination with other chemotherapeutics.
Funding source: Bulgarian National Science
Award Identifier / Grant number: DKOCT 01/27
Funding statement: This research was supported by the Bulgarian National Science Fund, Funder Id: http://dx.doi.org/10.13039/501100003336, Grant Number: DKOCT 01/27.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2019-0144).
©2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Research Articles
- Antibacterial and antifungal two phenolic sesquiterpenes from Dysoxylum densiflorum
- Isolation and identification of tuliposides D and F from tulip cultivars
- Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity
- Folate-conjugated Helix lucorum hemocyanin – preparation, stability, and cytotoxicity
- Comparative studies of the biological activities of selected herbal extracts and phenolic compounds isolated from Rosa gallica
- Ginsenosides, potent inhibitors of sialyltransferase
- Rapid communication
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Articles in the same Issue
- Frontmatter
- Research Articles
- Antibacterial and antifungal two phenolic sesquiterpenes from Dysoxylum densiflorum
- Isolation and identification of tuliposides D and F from tulip cultivars
- Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity
- Folate-conjugated Helix lucorum hemocyanin – preparation, stability, and cytotoxicity
- Comparative studies of the biological activities of selected herbal extracts and phenolic compounds isolated from Rosa gallica
- Ginsenosides, potent inhibitors of sialyltransferase
- Rapid communication
- Two new phenolic glycosides from the leaves of Garcinia epunctata Stapf
