Evaluation of anti-adipogenic active homoisoflavonoids from Portulaca oleracea
-
Jung Im Lee
and
Youngwan Seo
Abstract
This study was performed to isolate antiobesity components from the crude extract of Portulaca oleracea. The crude extract was partitioned into n-hexane, 85% aqueous methanol, n-butanol, and water fractions. Their effects on adipogenic differentiation were evaluated in 3T3-L1 cells. Among the solvent fractions from P. olearacea, the 85% aq. MeOH effectively reduced the levels of lipid accumulation. Further purification of 85% aq. MeOH led to the isolation of the known homoisoflavonoids 1–4, as the active substances. The administration of homoisoflavonoids to adipocyte cells decreased the lipid accumulation and glucose consumption and increased the release of glycerol into culture medium. In particular, homoisoflavonoid 3 effectively down-regulated the adipogenic transcription genes such as peroxisome proliferator activated receptor-γ (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPα), and adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid transport protein 1 (FATP1), and acyl-CoA synthase 1 (ACS1).
Acknowledgments
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1A2B4009588). Also, this work was supported by the National Research Foundation of Korea (NRF) and the Center for Women In Science, Engineering and Technology (WISET) Grant funded by the Ministry of Science and ICT (MSIT) under the Program for Returners into R&D (WISET-2018-792).
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Articles in the same Issue
- Frontmatter
- Special Section Articles
- The chemistry of social insects
- The critical role of primer pheromones in maintaining insect sociality
- Sexual attraction: a review of bumblebee male pheromones
- (3R,6E)-nerolidol, a fertility-related volatile secreted by the queens of higher termites (Termitidae: Syntermitinae)
- Regular Articles
- Evaluation of anti-adipogenic active homoisoflavonoids from Portulaca oleracea
- Structure elucidation and antimicrobial activities of five compounds from Artemisia integrifolia L
- Aromatase (CYP19) inhibition by biflavonoids obtained from the branches of Garcinia gardneriana (Clusiaceae)
Articles in the same Issue
- Frontmatter
- Special Section Articles
- The chemistry of social insects
- The critical role of primer pheromones in maintaining insect sociality
- Sexual attraction: a review of bumblebee male pheromones
- (3R,6E)-nerolidol, a fertility-related volatile secreted by the queens of higher termites (Termitidae: Syntermitinae)
- Regular Articles
- Evaluation of anti-adipogenic active homoisoflavonoids from Portulaca oleracea
- Structure elucidation and antimicrobial activities of five compounds from Artemisia integrifolia L
- Aromatase (CYP19) inhibition by biflavonoids obtained from the branches of Garcinia gardneriana (Clusiaceae)
