Abstract
Rhizopus microsporus var. oligosporus is a fungus that belongs to the Mucoraceae family that is used for the preparation of some soy-fermented foods. Microbial biotransformation of progesterone by R. microsporus var. oligosporus afforded some monohydroxylated and dihydroxylated metabolites. The main product was purified using chromatographic methods and identified as 11α-hydroxyprogesterone on the basis of its spectroscopic features. Time course studies by high-performance thin-layer chromatography demonstrated that this fungi efficiently hydroxylated progesterone at the 11α-position for 3 days with a yield of 76.48%, but beyond this time, the microorganism transformed 11α-hydroxyprogesterone into dihydroxylated metabolites. 11α-Hydroxyprogesterone is widely used as a precursor in the synthesis of hydrocortisone and other steroidal anti-inflammatory agents.
Acknowledgments
The authors are grateful to the School of Pharmacy, Shahid Beheshti University of Medical Sciences for providing essential facilities for this project as a part of M. Eslami’s Pharm. D. thesis.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Research Articles
- Biotransformation of a major beer prenylflavonoid – isoxanthohumol
- An efficient biotransformation of progesterone into 11α-hydroxyprogesterone by Rhizopus microsporus var. oligosporus
- Anticancer, antimicrobial and antioxidant potential of sterically tuned bis-N-heterocyclic salts
- On eccentricity-based topological descriptors of water-soluble dendrimers
- Topological characterization of dendrimer, benzenoid, and nanocone
- Analysis of the GC-MS of volatile compounds and the phytochemical profile and antioxidant activities of some Bulgarian medicinal plants
- Bacillus methylotrophicus ASWU-C2, a strain inhabiting hot desert soil, a new source for antibacterial bacillopyrone, pyrophen, and cyclopeptides
Articles in the same Issue
- Frontmatter
- Research Articles
- Biotransformation of a major beer prenylflavonoid – isoxanthohumol
- An efficient biotransformation of progesterone into 11α-hydroxyprogesterone by Rhizopus microsporus var. oligosporus
- Anticancer, antimicrobial and antioxidant potential of sterically tuned bis-N-heterocyclic salts
- On eccentricity-based topological descriptors of water-soluble dendrimers
- Topological characterization of dendrimer, benzenoid, and nanocone
- Analysis of the GC-MS of volatile compounds and the phytochemical profile and antioxidant activities of some Bulgarian medicinal plants
- Bacillus methylotrophicus ASWU-C2, a strain inhabiting hot desert soil, a new source for antibacterial bacillopyrone, pyrophen, and cyclopeptides