Effect of yeast extract addition to a mineral salts medium containing hydrolyzed plant xylan on fungal pullulan production
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Daniel E. Kennedy II
Abstract
The ability of the fungus Aureobasidium pullulans ATCC 42023 to produce pullulan from yeast extract-supplemented xylan hydrolysates of the prairie grass prairie cordgrass was examined relative to polysaccharide and cell biomass production, yield, and pullulan content of the polysaccharide. A pullulan concentration of 11.2 g L−1 and yield of 0.79 g g−1 was produced by ATCC 42023 when grown for 168 h at 30°C on the phosphate-buffered hydrolysate supplemented with yeast extract. The highest biomass level being 8.8 g L−1 was produced by ATCC 42023 after 168 h on a yeast extract-supplemented, hydrolysate-containing complete medium lacking sodium chloride. The highest pullulan content of the polysaccharide produced by ATCC 42023 after 168 h on the hydrolysate medium supplemented with yeast extract and ammonium sulfate was 70%. The findings indicate that a polysaccharide with a high pullulan content can be produced at a relatively high yield by the fungus grown on a yeast extract-supplemented xylan hydrolysate, suggesting that pullulan could be produced using a biomass-based process.
Acknowledgments
Financial support of this work was provided by the US Department of Agriculture grant no. 2003-35504-13622, no. 2005-35504-15460, NIFA Funder Id: 10.13039/100005825, grant no. 2011-67010-20051, and South Dakota Agricultural Experiment Station Grant SD00H434-12. The expert technical assistance of Jessica L. Peterson was greatly appreciated.
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Articles in the same Issue
- Frontmatter
- Research Articles
- PFGE: a tool for examination of heterogeneity between the bacterial spot-causing xanthomonads of tomato plants in Bulgaria
- Applications of magnetoliposomes with encapsulated doxorubicin for integrated chemotherapy and hyperthermia of rat C6 glioma
- Effect of the Arctic terrestrial plant Ranunculus hyperboreus on LPS-induced inflammatory response via MAPK pathways
- Cyanidin-3-rutinoside protects INS-1 pancreatic β cells against high glucose-induced glucotoxicity by apoptosis
- Research for the lichen Usnea barbata metabolites
- Shape-controlled synthesis of three-dimensional zinc oxide nanoflowers for disinfection of food pathogens
- Effects of two lichen acids isolated from Pseudevernia furfuracea (L.) Zopf in cultured human lymphocytes
- Letter
- Chemical composition and antifungal activity of essential oils from four Asteraceae plants grown in Egypt
- Research note
- Effect of yeast extract addition to a mineral salts medium containing hydrolyzed plant xylan on fungal pullulan production
