Abstract
Laurus nobilis leaves contain flavored and bioactive components with relevant biological properties for human health that are attributed to an abundant presence of highly bioactive secondary metabolites. However, the separation process for these bioactive molecules from plant matrix is seriously limited by the presence of a physical barrier (cell wall). Thus, the use of novel extraction procedures to enhance their release is particularly important. In this work, the potential use of microwave-assisted extraction (MAE) as a tool to improve the extraction efficiency of bioactive compounds from bay leaves and their characterization was evaluated. The effects of irradiation time (3, 6 and 9 min) and aqueous ethanol concentration (0, 25 and 50%) on the extraction of phenolic compounds were evaluated. A response surface methodology was applied to determine the best extraction conditions by MAE. The maximum total phenolic compound under the best conditions (9 min time irradiation and aqueous ethanol 50%) was 10.63±0.91 mg gallic acid equivalent/g plant using MAE. Also, the antioxidant potential of the extracts obtained was evaluated.
Acknowledgments
The authors thank the National Council of Science and Technology (CONACYT) Mexico for the financial support during their postgraduate studies at Universidad Autónoma de Coahuila. They also thank Coyotefoods, Biopolymer and Biotechnology S. de R.L. Mi. and MSc Claudia Patricia Flores Dávila for the facility, technical help and training in use of the microwaves.
References
1. Santoyo S, Lloria R, Jaime, L, Ibañez E. Supercritical fluid extraction of antioxidant and antimicrobial compounds from Laurus nobilis L. chemical and functional characterization. Eur Food Res Technol 2006;222:565–71.10.1007/s00217-005-0027-9Search in Google Scholar
2. Simić M, Kundaković T, Kovačević N. Preliminary assay on the antioxidative activity of Laurus nobilis extracts. Fitoterapia 2003;74:613–6.10.1016/S0367-326X(03)00143-6Search in Google Scholar
3. Elmastaş M, Gülcin İ, Isildak Ö, Küfrevioğlu Öİ, İbaoğlu K, Aboul-Enein HY. Radical scavenging activity and antioxidant capacity of bay leaf extracts. J Iran Chem Soc 2006;3:258–66.10.1007/BF03247217Search in Google Scholar
4. Pacifico S, Gallicchio M, Lorenz P, Potenza N, Galasso S, Marciano S, et al. Apolar Laurus nobilis leaf extracts induce cytotoxicity and apoptosis towards three nervous system cell lines. Food Chem Toxicol 2013;62:628–37.10.1016/j.fct.2013.09.029Search in Google Scholar PubMed
5. El-Chaghaby GA, Ahmad AF, Ramis ES. Evaluation of the antioxidant and antibacterial properties of various solvents extracts of Annona squamosa L. leaves. Arab J Chem 2014;7:227–33.10.1016/j.arabjc.2011.06.019Search in Google Scholar
6. Ballard TS, Mallikarjunan P, Zhou K, O’Keefe S. Microwave-assisted extraction of phenolic antioxidant compounds from peanut skins. Food Chem 2010;120:1185–92.10.1016/j.foodchem.2009.11.063Search in Google Scholar
7. Wang J, Sun B, Cao Y, Tian Y, Li X. Optimization of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chem 2008;106:804–10.10.1016/j.foodchem.2007.06.062Search in Google Scholar
8. Lucchesi ME, Chemat F, Smadja J. Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation. J Chromatogr A 2004;1043:323–7.10.1016/j.chroma.2004.05.083Search in Google Scholar PubMed
9. Sun Y, Liu Z, Wang J. Ultrasound-assisted extraction of five isoflavones from Iris tectorum Maxim. Sep Purif Technol 2011;78:49–54.10.1016/j.seppur.2011.01.017Search in Google Scholar PubMed PubMed Central
10. Yan MM, Liu W, Fu YJ, Zu YG, Chen CY, Luo M. Optimization of the microwave-assisted extraction process for four main astragalosides in Radix Astragali. Food Chem 2010;119: 1663–70.10.1016/j.foodchem.2009.09.021Search in Google Scholar
11. Muñiz DB, Martínez GC, Wong JE, Belmares R, Rodríguez R, Aguilar CN. Ultrasound-assisted extraction of phenolic compounds from Laurus nobilis L. and their antioxidant activity. Ultrason Sonochem 2013;20:1149–54.10.1016/j.ultsonch.2013.02.008Search in Google Scholar
12. Boulila A, Hassen I, Haouari L, Mejri F, Amor IB, Casabianca H, et al. Enzyme-assisted extraction of bioactive compounds from bay leaves (Laurus nobilis L.). Ind Crops Prod 2015;74:485–93.10.1016/j.indcrop.2015.05.050Search in Google Scholar
13. Hao L, Han W, Huang S, Xue B, Deng X. Microwave-assisted extraction of artemisinin from Artemisia annua. Sep Purif Technol 2002;28:191–6.10.1016/S1383-5866(02)00043-6Search in Google Scholar
14. Martins S, Aguilar CN, De la Garza I, Mussatto SI, Teixeira JA. Kinetic study of nordihydroguaiaretic acid recovery from Larrea tridentata by microwave-assisted extraction. J Chem Technol Biotechnol 2010;85:1142–7.10.1002/jctb.2412Search in Google Scholar
15. Song J, Li D, Liu C, Zhang Y. Optimized microwave-assisted extraction of total phenolics (TP) from Ipomoea batatas leaves and its antioxidant activity. Innov Food Sci Emer Technol 2011;12:282–7.10.1016/j.ifset.2011.03.001Search in Google Scholar
16. Pan G, Yu G, Zhu C, Qiao J. Optimization of ultrasound-assisted extraction (UAE) of flavonoids compounds (FC) from hawthorn seed (HS). Ultrason Sonochem 2012;19:486–90.10.1016/j.ultsonch.2011.11.006Search in Google Scholar PubMed
17. Galvan d’ A, Kriaa L, Nikov KI, Dimitrov K. Ultrasonic assisted extraction of polyphenols from black chokeberry. Sep Purif Technol 2012;93:42–7.10.1016/j.seppur.2012.03.024Search in Google Scholar
18. Li H, Deng Z, Wuc T, Liu R, Loewen S, Tsao R. Microwave-assisted extraction of phenolics with maximal antioxidant activities in tomatoes. Food Chem 2012;130:928–36.10.1016/j.foodchem.2011.08.019Search in Google Scholar
19. Upadhyay R, Ramalakshmi K, Mohan Rao J. Microwave-assisted extraction of chlorogenic acids from green coffee beans. Food Chem 2012;130:184–8.10.1016/j.foodchem.2011.06.057Search in Google Scholar
20. Cochard H, Nardini A, Coll L. Hydraulic architecture of leaf blades: where is the main resistance? Plant Cell Environ 2004;27:1257–67.10.1111/j.1365-3040.2004.01233.xSearch in Google Scholar
21. Wu T, Yan J, Liu R, Marcone MF, Aisa HA, Tsao R. Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design. Food Chem 2012;133:1292–8.10.1016/j.foodchem.2011.08.002Search in Google Scholar
22. Makkar H. Quantification of tannins in tree foliage. A laboratory manual. Vienna: Food and Agriculture Organization/International Atomic Energy Agency, 1999.Search in Google Scholar
23. Oliveira I, Sousa A, Ferreira I, Bento A, Estevinho L, Pereira JA. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food ChemToxicol 2008;46:2326–31.10.1016/j.fct.2008.03.017Search in Google Scholar PubMed
24. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211–9.Search in Google Scholar
25. Starzynska A, Stodolak B, Jamroz M. Antioxidant properties of extracts from fermented and cooked seeds of Polish cultivars of Lathyrus sativus. Food Chem 2008;109:285–92.10.1016/j.foodchem.2007.12.028Search in Google Scholar PubMed
26. Martínez CG, Aguilera AF, Rodríguez R, Aguilar CN. Fungal enhancement of the antioxidant properties of grape waste. Ann Microbiol 2011;62:922–30.10.1007/s13213-011-0329-zSearch in Google Scholar
©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Research Articles
- Diverse bioactive compounds from Sarcophtyton glaucom: structure elucidation and cytotoxic activity studies
- Pobeguinine: a monoterpene indole alkaloid and other bioactive constituents from the stem bark of Nauclea pobeguinii
- Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
- Evaluation of Lavandula stoechas L. subsp. stoechas L., Mentha spicata L. subsp. spicata L. essential oils and their main components against sinusitis pathogens
- Screening of the five different wild, traditional and industrial Saccharomyces cerevisiae strains to overproduce bioethanol in the batch submerged fermentation
- The effects of inulin and fructo-oligosaccharide on the probiotic properties of Lactobacillus spp. isolated from human milk
- A proteomics analysis of adventitious root formation after leaf removal in lotus (Nelumbo nucifera Gaertn.)
- Larval hemolymph of rhinoceros beetle, Allomyrina dichotoma, enhances insulin secretion through ATF3 gene expression in INS-1 pancreatic β-cells
- Antimalarial activity of the isolates from the marine sponge Hyrtios erectus against the chloroquine-resistant Dd2 strain of Plasmodium falciparum
- Rapid-communication
- Bioactive compounds from bay leaves (Laurus nobilis) extracted by microwave technology
- Erratum
- Erratum to: Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Erratum to: A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nucifera Gaertn)
Articles in the same Issue
- Frontmatter
- Research Articles
- Diverse bioactive compounds from Sarcophtyton glaucom: structure elucidation and cytotoxic activity studies
- Pobeguinine: a monoterpene indole alkaloid and other bioactive constituents from the stem bark of Nauclea pobeguinii
- Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
- Evaluation of Lavandula stoechas L. subsp. stoechas L., Mentha spicata L. subsp. spicata L. essential oils and their main components against sinusitis pathogens
- Screening of the five different wild, traditional and industrial Saccharomyces cerevisiae strains to overproduce bioethanol in the batch submerged fermentation
- The effects of inulin and fructo-oligosaccharide on the probiotic properties of Lactobacillus spp. isolated from human milk
- A proteomics analysis of adventitious root formation after leaf removal in lotus (Nelumbo nucifera Gaertn.)
- Larval hemolymph of rhinoceros beetle, Allomyrina dichotoma, enhances insulin secretion through ATF3 gene expression in INS-1 pancreatic β-cells
- Antimalarial activity of the isolates from the marine sponge Hyrtios erectus against the chloroquine-resistant Dd2 strain of Plasmodium falciparum
- Rapid-communication
- Bioactive compounds from bay leaves (Laurus nobilis) extracted by microwave technology
- Erratum
- Erratum to: Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Erratum to: A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nucifera Gaertn)