Home Larval hemolymph of rhinoceros beetle, Allomyrina dichotoma, enhances insulin secretion through ATF3 gene expression in INS-1 pancreatic β-cells
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Larval hemolymph of rhinoceros beetle, Allomyrina dichotoma, enhances insulin secretion through ATF3 gene expression in INS-1 pancreatic β-cells

  • Seung-Whan Kim , Hyun-Woo Suh , Bo-Kyung Yoo , Kisang Kwon , Kweon Yu , Ji-Young Choi and O-Yu Kwon EMAIL logo
Published/Copyright: May 22, 2018
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Abstract

In this study, we show that INS-1 pancreatic β-cells treated for 2 h with hemolymph of larvae of rhinoceros beetle, Allomyrina dichotoma, secreted about twice as much insulin compared to control cells without such treatment. Activating transcription factor 3 (ATF3) was the highest upregulated gene in DNA chip analysis. The A. dichotoma hemolymph dose-dependently induced increased expression levels of genes encoding ATF3 and insulin. Conversely, treatment with ATF3 siRNA inhibited expression levels of both genes and curbed insulin secretion. These results suggest that the A. dichotoma hemolymph has potential for treating and preventing diabetes or diabetes-related complications.

Acknowledgments

This study was supported by a grant (PJ010864) from the Agenda program funded by Rural Development Administration, Republic of Korea.

  1. Conflict of interest: The authors have no conflicts of interest related to this study to disclose.

References

1. Nowak V, Persijn D, Rittenschober D, Charrondiere UR. Review of food composition data for edible insects. Food Chem 2016;193:39–46.10.1016/j.foodchem.2014.10.114Search in Google Scholar PubMed

2. Yamada M, Nakamura K, Saido-Sakanaka H, Asaoka A, Yamakawa M, Sameshima T, et al. Effect of modified oligopeptides from the beetle Allomyrina dichotoma on E. coli infection in mice. J Vet Med 2004;66:137–42.10.1292/jvms.66.137Search in Google Scholar PubMed

3. Chung, MY, Yoon YI, Hwang JS, Goo TW, Yun EY. Anti-obesity effect of Allomyrinal dichotoma (Arthropoda: Insects) larvae ethanol extract on 27. 3T3-L1 adipocyte differentiation. Entomol Res 2014;44:9–16.10.1111/1748-5967.12044Search in Google Scholar

4. Miyanoshita A, Hara S, Sugiyama M, Asaoka A, Taniai K, Yukuhiro F, et al. Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma. Biochem Biophys Res Commun 1996;220:526–31.10.1006/bbrc.1996.0438Search in Google Scholar PubMed

5. Sagisaka A, Miyanoshita A, Ishibashi J, Yamakawa M. Purification, characterization and gene expression of a glycine and proline-rich antibacterial protein family from larvae of a beetle, Allomyrina dichotoma. Insect Mol Biol 2001;10:293–302.10.1046/j.0962-1075.2001.00261.xSearch in Google Scholar PubMed

6. Yoshikawa K, Umetsu K, Shinzawa H, Yuasa I, Maruyama K, Ohkura T, et al. Determination of carbohydrate-deficient transferring separated by lectin affinity chromatography for detecting chronic alcohol abuse. FEBS Lett 1996;458:112–6.10.1016/S0014-5793(99)01137-0Search in Google Scholar

7. Dushay MS. Insect hemolymph clotting. Cell Mol Life Sci 2009;66:2643–50.10.1007/s00018-009-0036-0Search in Google Scholar PubMed

8. Yoon YL, Chung MY, Hwang JS, Han MS, Goo TW, Yun EY. Allomyrina dichotoma (Arthropoda: Insecta) larvae confer resistance to obesity in mice fed a high-fat diet. Nutrients 2015;7:1978–91.10.3390/nu7031978Search in Google Scholar PubMed PubMed Central

9. Wang H, Jiang M, Cui H, Chen M, Buttyan R, Hayward SW, et al. The stress response mediator ATF3 represses androgen signaling by binding the androgen receptor. Mol Cell Biol 2012;32: 3190–202.10.1128/MCB.00159-12Search in Google Scholar PubMed PubMed Central

10. Wolford CC, McConoughey SJ, Jalgaonkar SP, Leon M, Merchant AS, Dominick JL, et al. Transcription factor ATF3 links host adaptive response to breast cancer metastasis. J Clin Invest 2013;123:2893–906.10.1172/JCI64410Search in Google Scholar PubMed PubMed Central

11. Hai T, Wolford CC, Chang YS. ATF3, a hub of the cellular adaptive-response network, in the pathogenesis of diseases: is modulation of inflammation a unifying component? Gene Expr 2010;15:1–11.10.3727/105221610X12819686555015Search in Google Scholar

12. Zhou H, Guo H, Zong J, Dai J, Yuan Y, Bian ZY, et al. ATF3 regulates multiple targets and may play a dual role in cardiac hypertrophy and injury. Int J Cardiol 2014;174:838–9.10.1016/j.ijcard.2014.04.160Search in Google Scholar PubMed

13. Kalfon R, Koren L, Aviram S, Schwartz O, Hai T, Aronheim A. ATF3 expression in cardiomyocytes preserves homeostasis in the heart and controls peripheral glucose tolerance. Cardiovasc Res 2017;113:134–46.10.1093/cvr/cvw228Search in Google Scholar PubMed

14. Kim JY, Park KJ, Hwang YJ, Kim GH, Lee D, Song EH, et al. Activating transcription factor 3 is a target molecule linking hepatic steatosis to impaired glucose homeostasis. J Hepatol 2017;S0168–8278:30187–93.10.1016/j.jhep.2017.03.023Search in Google Scholar PubMed

15. James CG, Woods A, Underhill TM, Beier F. The transcription factor ATF3 is upregulated during chondrocyte differentiation and represses cyclin D1 and A gene transcription. BMC Mol Biol 2006;7:1–11.10.1186/1471-2199-7-30Search in Google Scholar PubMed PubMed Central

16. Thompson M, Xu D, Williams BR. ATF3 transcription factor and its emerging roles in immunity and cancer. J Mol Med 2009;87:105360.10.1007/s00109-009-0520-xSearch in Google Scholar PubMed PubMed Central

17. Koh I, Lim JH, Joe MK, Kim WH, Jung MH, Yoon JB, et al. AdipoR2 is transcriptionally regulated by ER stress-inducible ATF3 in HepG2 human hepatocyte cells. FEBS J 2010;277:2304–17.10.1111/j.1742-4658.2010.07646.xSearch in Google Scholar PubMed

18. Zmuda EJ, Qi L, Zhu MX, Mirmira RG, Montminy MR, Hai T. The roles of ATF3, an adaptive-response gene, in high-fat-diet-induced diabetes and pancreatic beta-cell dysfunction. Mol Endocrinol 2010;24:1423–33.10.1210/me.2009-0463Search in Google Scholar PubMed PubMed Central

19. Jang MK, Jung MH. ATF3 inhibits PPARγ-stimulated transactivation in adipocyte cells. Biochem Biophys Res Commun 2015;456:80–5.10.1016/j.bbrc.2014.11.037Search in Google Scholar PubMed

20. Kim HB, Kong M, Kim TM, Suh YH, Kim WH, Lim JH, et al. ATF3 negatively regulates human AdipoR1 expression via binding to an ATF3-responsive region in the promoter NFATc4 and ATF3 negatively regulate adiponectin gene expression in 3T3-L1 adipocytes. Diabetes 2006;55:1342–52.10.2337/db05-1507Search in Google Scholar PubMed

21. Park HJ, Kang YM, Kim CH, Jung MH. ATF3 negatively regulates adiponectin receptor 1 expression. Biochem Biophys Res Commun 2010;400:72–7.10.1016/j.bbrc.2010.08.011Search in Google Scholar PubMed

22. Kim JY, Park KJ, Kim GH, Jeong EA, Lee DY, Lee SS, et al. In vivo activating transcription factor 3 silencing ameliorates the AMPK compensatory effects for ER stress-mediated β-cell dysfunction during the progression of type-2 diabetes. Cell Signal 2013;25:2348–61.10.1016/j.cellsig.2013.07.028Search in Google Scholar PubMed

Received: 2018-01-30
Revised: 2018-03-27
Accepted: 2018-04-16
Published Online: 2018-05-22
Published in Print: 2018-09-25

©2018 Walter de Gruyter GmbH, Berlin/Boston

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