Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
-
Alena Komersová
and
Alexander Čegan
Abstract
The dependence of the activity of acetylcholinesterase from electric eel at a pH value range of 4.8–9.8 (phosphate buffer), regarding acetylcholine and acetylthiocholine hydrolysis, was determined at 25 °C, ionic strength of 0.11 M, and initial substrate concentration of 4 mM. At a pH range of 4.8–9.8, the dependences A(pH) form a sigmoid increasing curve with the maximum catalytic activity at a pH range 8–9.5. For acetylcholine hydrolysis, the kinetic reason for such an increase in A consists mainly of an increase in the rate constant k2 (Michaelis-Menten) model with increasing pH of the reaction mixture. For acetylthiocholine hydrolysis, the kinetic explication of the determined dependence A(pH) is more complicated.
Funding source: Ministry of Education
Award Identifier / Grant number: SGS_2017_007
Funding statement: This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic no. SGS_2017_007.
Conflict of interest statement: The authors report no declarations of interest.
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Articles in the same Issue
- Frontmatter
- Research Articles
- Diverse bioactive compounds from Sarcophtyton glaucom: structure elucidation and cytotoxic activity studies
- Pobeguinine: a monoterpene indole alkaloid and other bioactive constituents from the stem bark of Nauclea pobeguinii
- Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
- Evaluation of Lavandula stoechas L. subsp. stoechas L., Mentha spicata L. subsp. spicata L. essential oils and their main components against sinusitis pathogens
- Screening of the five different wild, traditional and industrial Saccharomyces cerevisiae strains to overproduce bioethanol in the batch submerged fermentation
- The effects of inulin and fructo-oligosaccharide on the probiotic properties of Lactobacillus spp. isolated from human milk
- A proteomics analysis of adventitious root formation after leaf removal in lotus (Nelumbo nucifera Gaertn.)
- Larval hemolymph of rhinoceros beetle, Allomyrina dichotoma, enhances insulin secretion through ATF3 gene expression in INS-1 pancreatic β-cells
- Antimalarial activity of the isolates from the marine sponge Hyrtios erectus against the chloroquine-resistant Dd2 strain of Plasmodium falciparum
- Rapid-communication
- Bioactive compounds from bay leaves (Laurus nobilis) extracted by microwave technology
- Erratum
- Erratum to: Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Erratum to: A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nucifera Gaertn)
