Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity
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Akash P. Bhangale
Akash P. Bhangale working at former Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Sushant D. Wadekar working at former Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Sandeep B. Kale Professor, at DBT–ICT Centre for Energy Biosciences, Department of Chemical Engineering, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Suraj N. Mali completed his M. Pharm (Pharmaceutical Chemistry) at Institute of Chemical Technology, Mumbai in 2019. He has completed his B. Pharm at Government College of Pharmacy, Karad, India. Then, he joined Dr. Reddy’s Lab, Hyderabad as analytical scientist. He has
53+ international publications of repute (Citations:383 ; H-index:12 ) at his credit in the field of drug discovery, medicinal chemistry, computer-aided drug design, and synthetic/organic chemistry.Amit P. Pratap completed his graduation and post-graduation in Oil Technology in 2001 and obtained his doctorate degree in 2006 from Institute of Chemical Technology, Mumbai. He served the department as a ‘Professor J. G. Kane Academic Associate’ for over two years and worked as Assistant Professor over 10 years. He is currently a professor and head of the department of oils, oleochemicals and surfactant technology. For more than 20 years he is involved in the teaching, research and development in the field of vegetable oil-based lubricants, additives and biosurfactants. At present he is working as Professor and head, Department of Oils, Oleochemicals and Surfactants Technology. Experience over 20 years instead of 15 years. His research interest includes triboapplications of vegetable oils, structural modifications of oils and fats, biosurfactants and specialty products.
Abstract
Among glycolipids, mannosylerythritol lipids (MEL), are mild and environmentally friendly surfactants used in various industrial applications. MELs are produced by biofermentation using non-traditional oils with various water-soluble carbon sources as cell growth booster. This substrate affects the production yield and cost of MEL. In this research work, the non-traditional oils jatropha oil, karanja oil and neem oil were used as new substrates along with glucose, glycerol and honey as new water-soluble substrates. All these oils are new feedstocks for the production of MEL using Pseudozyma antarctica (ATCC 32657). Jatropha oil, karanja oil and neem oil with honey as substrates resulted in higher MEL yields of (8.07, 7.75, and 1.86) g/L and better cell growth of (8.07, 7.75, and 1.86) g/L, respectively, than non-traditional oils with glucose and glycerol as substrates. Neem oil gave a lower yield of MEL (1.54 g/L) as well as cell growth (6.06 g/L) compared to jatropha oil and karanja oil (7.03 and 6.17) g/L, respectively. Crude MEL from the fermentation broth was detected by thin-layer chromatography (TLC), Fourier transform infrared spectrommetry (FT-IR), high performance liquid chromatography (HPLC) and proton nuclear magnetic resonance spectroscopy (1H NMR). Purified MEL has been used as an antimicrobial agent in cosmetic products associated with gram-positive and gram-negative bacteria and fungi.
About the authors
Akash P. Bhangale working at former Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Sushant D. Wadekar working at former Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Sandeep B. Kale Professor, at DBT–ICT Centre for Energy Biosciences, Department of Chemical Engineering, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/ UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400 019, India.
Suraj N. Mali completed his M. Pharm (Pharmaceutical Chemistry) at Institute of Chemical Technology, Mumbai in 2019. He has completed his B. Pharm at Government College of Pharmacy, Karad, India. Then, he joined Dr. Reddy’s Lab, Hyderabad as analytical scientist. He has 53+ international publications of repute (Citations: 383; H-index: 12) at his credit in the field of drug discovery, medicinal chemistry, computer-aided drug design, and synthetic/organic chemistry.
Amit P. Pratap completed his graduation and post-graduation in Oil Technology in 2001 and obtained his doctorate degree in 2006 from Institute of Chemical Technology, Mumbai. He served the department as a ‘Professor J. G. Kane Academic Associate’ for over two years and worked as Assistant Professor over 10 years. He is currently a professor and head of the department of oils, oleochemicals and surfactant technology. For more than 20 years he is involved in the teaching, research and development in the field of vegetable oil-based lubricants, additives and biosurfactants. At present he is working as Professor and head, Department of Oils, Oleochemicals and Surfactants Technology. Experience over 20 years instead of 15 years. His research interest includes triboapplications of vegetable oils, structural modifications of oils and fats, biosurfactants and specialty products.
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Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: None declared.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Novel Surfactants
- Synthesis and characterisation of carboxy amide-bonded pyridinium Gemini surfactants: influence of the nature of the spacer group and counterions on the aggregation behaviour
- Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity
- Physical Chemistry
- Synergistic behavior of SLS-OPE-10 binary mixtures at their CMC
- Synergistic interaction between sodium dodecyl benzene sulfonate (SDBS) and N,N-dimethyldodecan-1-amine oxide (DDAO) and their adsorption onto activated charcoal and Jordanian natural clay
- Investigation of the synergistic effect and the morphology of the binary compound systems with potassium N-lauroyl glycinate
- Textile Cleaning
- Effect of the pre-treatment of stains with ultrasound in household laundry cleaning
- Flotation
- Effect of detergents on froth stability and flotation separation
- Environmental Chemistry
- Stability of pesticide in water emulsion induced by mixed surfactants
- Synthesis of surface active agents from natural waste phenolics
Articles in the same Issue
- Frontmatter
- Novel Surfactants
- Synthesis and characterisation of carboxy amide-bonded pyridinium Gemini surfactants: influence of the nature of the spacer group and counterions on the aggregation behaviour
- Non-traditional oils with water-soluble substrate as cell growth booster for the production of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657) with their antimicrobial activity
- Physical Chemistry
- Synergistic behavior of SLS-OPE-10 binary mixtures at their CMC
- Synergistic interaction between sodium dodecyl benzene sulfonate (SDBS) and N,N-dimethyldodecan-1-amine oxide (DDAO) and their adsorption onto activated charcoal and Jordanian natural clay
- Investigation of the synergistic effect and the morphology of the binary compound systems with potassium N-lauroyl glycinate
- Textile Cleaning
- Effect of the pre-treatment of stains with ultrasound in household laundry cleaning
- Flotation
- Effect of detergents on froth stability and flotation separation
- Environmental Chemistry
- Stability of pesticide in water emulsion induced by mixed surfactants
- Synthesis of surface active agents from natural waste phenolics
