Preparation and radiolabeling of Iranian Androctonus crassicauda scorpion venom with technetium-99m as a new agent for cancer diagnostic and therapeutic purposes
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Leila Valipour Yekany
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Mostafa Erfani
Abstract
One of the innovative methods in cancer treatment with fewer side effects is the use of active principles produced by animals and microorganisms to generate new drugs with diagnosis and treatment capability for cancer. Androctonus crassicauda scorpion venom has high potential as an anti-tumor agent and has the ability to interfere with human physiology but is still unknown. Therefore, labeling venom with radionuclides is very important to study the biological distribution of toxins for diagnostic and therapeutic purposes of various diseases, especially cancer. In this research, the toxic fraction of this venom was labeled with 99mTc. The radiochemical purity of the labeled toxic fraction was obtained by using chromatographic systems. Animal biodistribution studies were performed after injecting the labeled compound into normal rats and 4T1 breast cancer-bearing mice. The radiochemical purity of 90% was obtained for labeled compound. Biological distribution studies showed relatively rapid clearance of the labeled compound from the blood circulation system. The accumulation of the labeled compound in the liver and kidney was the highest among the organs, which could indicate the possibility of toxin excretion metabolism from liver and the kidneys. The accumulation rate of the labeled compound after 15 min was 7% in the breast cancer mass, and the ratio of the distribution of the labeled compound in the breast cancer mass to the blood was 155% at 15 min post injection. The study results indicated that scorpion venom labeling with 99mTc could be a useful tool for the biodistribution and kinetic studies of the venoms for clinical use. On the other hand, based on the results of the biological distribution of the labeled compound in tumor-bearing mice, the 99mTc-venom complex can be used as an imaging agent to diagnose breast cancer. Moreover at the same time, with some measures, it can be used as a therapeutic agent for breast cancer with more consideration. We hope that the results of this study will be a step in the future for the clinical diagnostic and therapeutic purposes of cancer using natural products.
Acknowledgment
The authors wish to thank all staff of the institute and the radiation application school for their contribution in the research and also Mr. Mazidi, Mr. Parayandeh, Mr. Goodarzi and Mr. Karami for their cooperation, and Mr. Amoosi to edit the English grammar.
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Author contributions: LVY, FBM, SPS, ME and AZM designed the study. LVY, SPS, ME, and AZM collected the data. LVY processed the data. LVY, FBM and SPS interpreted the data. SPS drafted and revised the manuscript. All authorsread and approved the final manuscript.
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Research funding: Not applicable.
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Conflict of interest statement: The authors declare that they have no competing interests.
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Availability of data and materials: The datasets used and analysed during the current study are available from the corresponding author on reasonablerequest.
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Ethics approval and consent to participate: Animal experiments were performed in compliance with the regulations of our institution and with generally accepted guidelines governing such work (Code no. NSTRI-1400).
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Consent for publication: Not applicable.
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Papers
- Experimental cross section of the 164Dy(n,γ)165Dy reaction at the neutron energy of 0.0372 eV using Neutron Diffraction Facility
- Is octavalent plutonium really formed during oxidation in alkaline aqueous solutions?
- A comparison of the extraction behaviour of tris(2-methylbutyl) phosphate and tri-n-alkyl phosphates for the separation of metal ions for U–Zr and U–Pu–Zr systems by cross-current mode
- Investigating diffusion mechanism for HTO and Se(IV)/Se(VI) in compacted Tamusu clay rock with different column lengths
- Preparation and radiolabeling of Iranian Androctonus crassicauda scorpion venom with technetium-99m as a new agent for cancer diagnostic and therapeutic purposes
- Fabrication and application of nanosized stannic oxide for sorption of some hazardous metal ions from aqueous solutions
Articles in the same Issue
- Frontmatter
- Original Papers
- Experimental cross section of the 164Dy(n,γ)165Dy reaction at the neutron energy of 0.0372 eV using Neutron Diffraction Facility
- Is octavalent plutonium really formed during oxidation in alkaline aqueous solutions?
- A comparison of the extraction behaviour of tris(2-methylbutyl) phosphate and tri-n-alkyl phosphates for the separation of metal ions for U–Zr and U–Pu–Zr systems by cross-current mode
- Investigating diffusion mechanism for HTO and Se(IV)/Se(VI) in compacted Tamusu clay rock with different column lengths
- Preparation and radiolabeling of Iranian Androctonus crassicauda scorpion venom with technetium-99m as a new agent for cancer diagnostic and therapeutic purposes
- Fabrication and application of nanosized stannic oxide for sorption of some hazardous metal ions from aqueous solutions
