Home Physical Sciences Uranium biosorption by immobilized active yeast cells entrapped in calcium-alginate-PVA- GO-crosslinked gel beads
Article
Licensed
Unlicensed Requires Authentication

Uranium biosorption by immobilized active yeast cells entrapped in calcium-alginate-PVA- GO-crosslinked gel beads

  • Can Chen , Jun Hu and Jianlong Wang EMAIL logo
Published/Copyright: September 17, 2019
Radiochimica Acta
From the journal Radiochimica Acta Volume 108 Issue 4

Abstract

A novel biosorbent, i. e. Saccharomyces cerevisiae entrapped in graphene oxide (GO), polyvinyl alcohol (PVA) and alginate and cross-linked in CaCl2- boric acid solution, was prepared, characterized and applied for U (VI) biosorption. The performance of U sorption and cations release (Na, K, Ca and Mg ions) was investigated under different contact time, initial uranium concentration and initial pH. Uranium sorption equilibrium basically achieved after 360 min. The kinetic data of U biosorption and Ca release were best described by the pseudo first-order equation. Both Langmuir and Freundlich models could fit the U sorption isotherm data. With increase of initial uranium (3.7 ~ 472.2 μmol/L) and sodium concentration (78.8 ~ 3911.7 μmol/L), the cations release ((Na + K)/2 + (Ca + Mg)) decreased from 116.9 to 30.1 μmol/g when the corresponding U sorption increased from 0.6 to 77.3 μmol/g. Initial solution pH at 3 was favorable for U sorption when pH ranged from 3 to 7. With increase of uranium concentration, ion exchange played a less role in U removal. The maximum U sorption capacity reached 142.1 μmol/g, calculated from the Langmuir model at initial pH 5. The O-containing functional group, such as carboxyl on the gel bead played an important role in U adsorption according to FTIR and XPS analysis. XPS analysis showed the existence of U (VI) and U (IV) on the surface of gel bead. Ion exchange, complexation and uranium reduction involved in uranium adsorption by the immobilized active dry yeast gel beads.

Keywords: Uranium; yeast; gel beads; biosorption; mechanism
Corresponding author: Prof. Jianlong Wang, Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, P.R. China; and Beijing Key Laboratory of Radioactive Waste Treatment, Energy Science Building, Tsinghua University, Beijing 100084, P.R. China, Tel.: +86 10 62784843, Fax: +86 10 62771150

Funding source: National Key Research and Development Program

Award Identifier / Grant number: 2016YFC1402507

Funding source: National S&T Major Project

Award Identifier / Grant number: 2013ZX06002001

Funding source: Program for Changjiang Scholars and Innovative Research Team in University

Award Identifier / Grant number: IRT-13026

Funding statement: The research was supported by the National Key Research and Development Program (2016YFC1402507), the National S&T Major Project (2013ZX06002001) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT-13026).

References

1. Wang, J. L., Zhuang, S. T.: Extraction and adsorption of U(VI) from aqueous solution using affinity ligand-based technologies: an overview. Rev. Environ. Sci. Biotechnol. 18, 437 (2019).10.1007/s11157-019-09507-ySearch in Google Scholar

2. Zhuang, S. T., Wang, J. L.: Removal of U(VI) from aqueous solution using phosphate functionalized bacterial cellulose as efficient adsorbent. Radiochim. Acta 107, 459 (2019).10.1515/ract-2018-3077Search in Google Scholar

3. Wang, J. L., Chen C.: Chitosan-based biosorbents: modification and application for biosorption of heavy metals and radionuclides. Bioresour. Technol. 160, 129 (2014).10.1016/j.biortech.2013.12.110Search in Google Scholar PubMed

4. Wang, J. L., Zhuang, S. T., Liu, Y.: Metal hexacyanoferrates-based adsorbents for cesium removal. Coord. Chem. Rev. 374, 430 (2018).10.1016/j.ccr.2018.07.014Search in Google Scholar

5. Wang, J. L., Zhuang, S. T.: Cesium separation from radioactive waste by extraction and adsorption based on crown ethers and calixarenes. Nucl. Eng. Technol. (2019). doi: https://doi.org/10.1016/j.net.2019.08.001.10.1016/j.net.2019.08.001Search in Google Scholar

6. Chen, Y. W., Wang, J. L.: Removal of radionuclide Sr2+ ions from aqueous solution using synthesized magnetic chitosan beads. Nucl. Eng. Des. 242, 445 (2012).10.1016/j.nucengdes.2011.10.059Search in Google Scholar

7. Xing, M., Xu, L. J., Wang, J. L.: Mechanism of Co(II) adsorption by zero valent iron/graphene nanocomposite. J. Hazard. Mater. 301, 286 (2016).10.1016/j.jhazmat.2015.09.004Search in Google Scholar PubMed

8. Wang, J. L., Zhuang, S. T.: Removal of various pollutants from water and wastewater by modified chitosan adsorbents. Crit. Rev. Environ. Sci. Technol. 47, 2331 (2017).10.1080/10643389.2017.1421845Search in Google Scholar

9. Wang, J. L., Zhuang, S. T.: Removal of cesium ions from aqueous solutions using various separation technologies. Rev. Environ. Sci. Biotechnol. 18, 231 (2019).10.1007/s11157-019-09499-9Search in Google Scholar

10. Wang, J. L., Chen, C.: Biosorption of heavy metals by Saccharomyces cerevisiae: a review. Biotechnol. Adv. 24, 427 (2006).10.1016/j.biotechadv.2006.03.001Search in Google Scholar PubMed

11. Chen, C., Wang, J. L.: Uranium removal by novel graphene oxide-immobilized Saccharomyces cerevisiae gel beads. J. Environ. Radioact. 162, 134 (2016).10.1016/j.jenvrad.2016.05.012Search in Google Scholar

12. Zhang, L. S., Wu, W. Z., Wang, J. L.: Immobilization of activated sludge using improved polyvinyl alcohol (PVA) gel. J. Environ. Sci. 19, 1293 (2007).10.1016/S1001-0742(07)60211-3Search in Google Scholar

13. Wang, J. L., Liu, P., Qian, Y.: Biodegradation of phthalic acid esters by immobilized microbial cells. Environ. Int. 23, 775 (1997).10.1016/S0160-4120(97)00089-5Search in Google Scholar

14. Wang, J. L., Chen C.: Biosorbents for heavy metals removal and their future. Biotechnol. Adv. 27, 195 (2009).10.1016/j.biotechadv.2008.11.002Search in Google Scholar

15. Wang, J. L., Quan, X. C., Han, L. P., Qian, Y., Werner, H.: Microbial degradation of quinoline by immobilized cells of Burkholderia pickettii. Water Res. 36, 2288 (2002).10.1016/S0043-1354(01)00457-2Search in Google Scholar

16. Brady, D., Duncan, J. R.: Cation loss during accumulation of heavy-metal cations by Saccharomyces cerevisiae. Biotechnol. Lett. 16, 543 (1994).10.1007/BF01023341Search in Google Scholar

17. Chen, C., Wang, J. L.: Characteristics of Zn2+ biosorption by Saccharomyces cerevisiae. Biomed. Environ. Sci. 20, 478 (2007).Search in Google Scholar

18. Liang, J., Huang, Y., Zhang, L., Wang, Y., Ma, Y., Guo, T., Chen, Y.: Molecular-level dispersion of graphene into poly (vinyl alcohol) and effective reinforcement of their nanocomposites. Adv. Funct. Mater. 19, 2297 (2009).10.1002/adfm.200801776Search in Google Scholar

19. Zhang, L., Wang, Z., Xu, C., Li, Y., Gao, J., Wang, W., Liu, Y.: High strength graphene oxide/polyvinyl alcohol composite hydrogels. J. Mater. Chem. 21, 10399 (2011).10.1039/c0jm04043fSearch in Google Scholar

20. Bai, H., Li, C., Wang, X., Shi, G.: A pH-sensitive graphene oxide composite hydrogel. Chem. Commun. 46, 2376 (2010).10.1039/c000051eSearch in Google Scholar

21. Prakasham, R. S., Kuriakose, B., Ramakrishna, S. V.: The influence of inert solids on ethanol production by Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 82, 127 (1999).10.1385/ABAB:82:2:127Search in Google Scholar

22. Ho, Y. S.: Review of second-order models for adsorption systems. J. Hazard. Mater. 136, 681 (2006).10.1016/j.jhazmat.2005.12.043Search in Google Scholar

23. Ho, Y. S., McKay, G.: Pseudo-second order model for sorption processes. Process Biochem. 34, 451 (1999).10.1016/S0032-9592(98)00112-5Search in Google Scholar

24. Plazinski, W., Rudzinski, W., Plazinska, A.: Theoretical models of sorption kinetics including a surface reaction mechanism: a review. Adv. Colloid Interface Sci. 152, 2 (2009).10.1016/j.cis.2009.07.009Search in Google Scholar PubMed

25. Largitte, L., Pasquier, R.: A review of the kinetics adsorption models and their application to the adsorption of lead by an activated carbon. Chem. Eng. Res. Des. 109, 495 (2016).10.1016/j.cherd.2016.02.006Search in Google Scholar

26. Bai, J., Li, Z., Fan, F. L., Wu, X. L., Tian, W., Yin, X. J., Zhao, L., Fan, F. Y., Tian, L. L., Wang, Y., Qin, Z., Guo, J. S.: Biosorption of uranium by immobilized cells of Rhodotorula glutinis. J. Radioanal. Nucl. Chem. 299, 1517 (2014).10.1007/s10967-013-2900-5Search in Google Scholar

27. Yi, Z. J., Yao, J., Zhu, M. J., Chen, H. L., Wang, F., Liu, X.: Biosorption characteristics of Ceratophyllum demersum biomass for removal of uranium (VI) from an aqueous solution. J. Radioanal. Nucl. Chem. 313, 19 (2017).10.1007/s10967-017-5269-zSearch in Google Scholar

28. Yi, Z. J., Yao, J., Zhu, M. J., Chen, H. L., Wang, F., Liu, X.: Uranium biosorption from aqueous solution by the submerged aquatic plant Hydrilla verticillata. Water Sci. Technol. 75, 1332 (2017).10.2166/wst.2016.592Search in Google Scholar PubMed

29. Caccin, M., Giacobbo, F., Da Ros, M., Besozzi, L., Mariani, M.: Adsorption of uranium, cesium and strontium onto coconut shell activated carbon. J. Radioanal. Nucl. Chem. 297, 9 (2013).10.1007/s10967-012-2305-xSearch in Google Scholar

30. Sreejalekshmi, K. G., Krishnan, K. A., Anirudhan, T. S.: Adsorption of Pb(II) and Pb(II)-citric acid on sawdust activated carbon: kinetic and equilibrium isotherm studies. J. Hazard. Mater. 161, 1506 (2009).10.1016/j.jhazmat.2008.05.002Search in Google Scholar PubMed

31. Lin, C. C., Lai, Y. T.: Adsorption and recovery of lead (II) from aqueous solutions by immobilized Pseudomonas aeruginosa PU21 beads. J. Hazard. Mater. 137, 99 (2006).10.1016/j.jhazmat.2006.02.071Search in Google Scholar PubMed

32. Mohanty, K., Das, D., Biswas, M. N.: Treatment of phenolic wastewater in a novel multi-stage external loop airlift reactor using activated carbon. Sep. Purif. Technol. 58, 311 (2008).10.1016/j.seppur.2007.05.005Search in Google Scholar

33. Guo, X., Wang, J. L.: A general kinetic model for adsorption: theoretical analysis and modeling. J. Mol. Liq. 288, 111100 (2019).10.1016/j.molliq.2019.111100Search in Google Scholar

34. Guo, X., Wang, J. L.: The phenomenological mass transfer kinetics model for Sr2+ sorption onto spheroids primary microplastics. Environ. Pollut. 250, 737 (2019).10.1016/j.envpol.2019.04.091Search in Google Scholar PubMed

35. Wang, J. S., Hu, X. J., Liu, Y. G., Xie, S. B., Bao, Z. L.: Biosorption of uranium (VI) by immobilized Aspergillus fumigatus beads. J. Environ. Radioact. 101, 504 (2010).10.1016/j.jenvrad.2010.03.002Search in Google Scholar PubMed

36. Yin, H., He, B., Peng, H., Ye, J., Yang, F., Zhang, N.: Removal of Cr (VI) and Ni (II) from aqueous solution by fused yeast: study of cations release and biosorption mechanism. J. Hazard. Mater. 158, 568 (2008).10.1016/j.jhazmat.2008.01.113Search in Google Scholar PubMed

37. Liu, W., Dai, X., Bai, Z., Wang, Y., Yang, Z., Zhang, L., Xu, L., Chen, L., Li, Y., Gui, D., Juan, D., Wang, J., Zhou, R., Chai, Z., Wang, S. A.: Highly sensitive and selective uranium detection in natural water systems using a luminescent mesoporous metal-organic framework equipped with abundant Lewis basic sites: a combined batch, X-ray absorption spectroscopy, and first principles simulation investigation. Environ. Sci. Technol. 51, 3911 (2017).10.1021/acs.est.6b06305Search in Google Scholar PubMed

38. Zheng, T., Yang, Z. X., Gui, D. X., Liu, Z. Y., Wang, X. X., Dai, X., Liu, S. T., Zhang, L. J., Gao, Y., Chen, L. H., Sheng, D. P., Wang, Y. L., Juan, D. W., Wang, J. Q., Zhou, R. H., Chai, Z. F., Albrecht-Schmitt, T. E., Wang, S. A.: Overcoming the crystallization and designability issues in the ultrastable zirconium phosphonate framework system. Nat. Commun. 8, 15369 (2017).10.1038/ncomms15369Search in Google Scholar PubMed PubMed Central

39. Li, H., Zhai, F. Z., Gui, D. X., Wang, X. X., Wu, C. F., Zhang, D., Dai, X., Deng, H., Su, X. T., Di, W. J., Lin, Z., Chai, Z. F., Wang, S.: Powerful uranium extraction strategy with combined ligand complexation and photocatalytic reduction by postsynthetically modified photoactive metal-organic frameworks. Appl. Catal. B: Environ. 254, 47 (2019).10.1016/j.apcatb.2019.04.087Search in Google Scholar

40. Hu, M., Reeves, M.: Biosorption of uranium by Pseudomonas aeruginosa strain CSU immobilized in a novel matrix. Biotechnol. Prog. 13, 60 (1997).10.1021/bp9600849Search in Google Scholar

41. Wang, Y. S., Chen, Y. T., Liu, C., Yu, F.: High-efficiency enrichment of uranium (VI) from aqueous solution by hydromagnesite and its calcination products. J. Radioanal. Nucl. Chem. 315, 171 (2018).10.1007/s10967-017-5661-8Search in Google Scholar

42. D’Souza, S. F., Sar, P., Kazy, S. K., Kubal, B. S.: Uranium sorption by Pseudomonas biomass immobilized in radiation polymerized polyacrylamide bio-beads. J. Environ. Sci. Health Part A. 41, 487 (2006).10.1080/10934520500428377Search in Google Scholar PubMed

43. Liu, M. X., Dong, F. Q., Yan, X. Y., Zeng, W. M., Hou, L. Y., Pang, X. F.: Biosorption of uranium by Saccharomyces cerevisiae and surface interactions under culture conditions. Bioresour. Technol. 101, 8573 (2010).10.1016/j.biortech.2010.06.063Search in Google Scholar PubMed

44. Vazquez-Campos, X., Kinsela, A. S., Collins, R. N., Neilan, B. A., Aoyagi, N., Waite, T. D.: Uranium binding mechanisms of the acid-tolerant fungus Coniochaeta fodinicola. Environ. Sci. Technol. 49, 8487 (2015).10.1021/acs.est.5b01342Search in Google Scholar PubMed

45. Yi, Z. J., Yao, J., Chen, H. L., Wang, F., Yuan, Z. M., Liu, X.: Uranium biosorption from aqueous solution onto Eichhornia crassipes. J. Environ. Radioact. 154, 43 (2016).10.1016/j.jenvrad.2016.01.012Search in Google Scholar PubMed

46. Kushwaha, S., Sreedhar, B., Padmaja, P.: XPS, EXAFS, and FTIR as tools to probe the unexpected adsorption-coupled reduction of U(VI) to U(V) and U(IV) on Borassus flabellifer-based adsorbents. Langmuir. 28, 16038 (2012).10.1021/la3013443Search in Google Scholar PubMed

47. Scott, T. B., Allen, G. C., Heard, P. J., Randell, M. G.: Reduction of U (VI) to U (IV) on the surface of magnetite. Geochim. Cosmochim. Acta 69, 5639 (2005).10.1016/j.gca.2005.07.003Search in Google Scholar

48. Zheng, X., Wang, X., Shen, Y., Lu, X., Wang, T.: Biosorption and biomineralization of uranium (VI) by Saccharomyces cerevisiae-Crystal formation of chernikovite. Chemosphere 175, 161 (2017).10.1016/j.chemosphere.2017.02.035Search in Google Scholar PubMed

49. Veleshko, A. N., Veleshko, I. E., Teterin, A. Y., Maslakov, K. I., Vukcevic, L., Teterin, Y. A., Ivanov, K. E.: An x-ray photoelectron spectroscopy study of uranyl–chitosan interaction. Nucl. Technol. Radiat. Prot. 23, 43 (2008).10.2298/NTRP0802043VSearch in Google Scholar

50. Sevilla, M., Fuertes, A. B.: Chemical and structural properties of carbonaceous products obtained by hydrothermal carbonization of saccharides. Chem. Eur. J. 15, 4195 (2009).10.1002/chem.200802097Search in Google Scholar PubMed

51. Ni, M., Ratner, B. D.: Differentiating calcium carbonate polymorphs by surface analysis techniques – an XPS and TOF-SIMS study. Surf. Interface Anal. 40, 1356 (2008).10.1002/sia.2904Search in Google Scholar PubMed PubMed Central

52. Cuif, J. P., Bendounan, A., Dauphin, Y., Nouet, J., Sirotti, F.: Synchrotron-based photoelectron spectroscopy provides evidence for a molecular bond between calcium and mineralizing organic phases in invertebrate calcareous skeletons. Anal. Bioanal. Chem. 405, 8739 (2013).10.1007/s00216-013-7312-4Search in Google Scholar PubMed

Received: 2019-03-30
Accepted: 2019-08-12
Published Online: 2019-09-17
Published in Print: 2020-03-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Effect of ionic liquid on the extraction of uranium with pillar[5]arene-based phosphine oxide from nitric acid solutions
  3. Topological analysis of the layered uranyl compounds bearing slabs with UO2:TO4 ratio of 2:3
  4. Adsorption of uranium from its aqueous solutions using activated cellulose and silica grafted cellulose
  5. Uranium biosorption by immobilized active yeast cells entrapped in calcium-alginate-PVA- GO-crosslinked gel beads
  6. Sorption of cesium on Tamusu clay in synthetic groundwater with high ionic strength
  7. Optimization of 14C LSC measurement using CO2 absorption technique
  8. Targeting 5α-reductase with 99mTc labeled dutasteride derivatives for prostate imaging
  9. Changes in structural and optical properties due to γ-irradiation of MgO nanoparticles
  10. Radiation-induced free radicals from different milk powders and its possible use as radiation dosimeters
https://doi.org/10.1515/ract-2019-3150
Keywords for this article
Uranium; yeast; gel beads; biosorption; mechanism

Articles in the same Issue

  1. Frontmatter
  2. Effect of ionic liquid on the extraction of uranium with pillar[5]arene-based phosphine oxide from nitric acid solutions
  3. Topological analysis of the layered uranyl compounds bearing slabs with UO2:TO4 ratio of 2:3
  4. Adsorption of uranium from its aqueous solutions using activated cellulose and silica grafted cellulose
  5. Uranium biosorption by immobilized active yeast cells entrapped in calcium-alginate-PVA- GO-crosslinked gel beads
  6. Sorption of cesium on Tamusu clay in synthetic groundwater with high ionic strength
  7. Optimization of 14C LSC measurement using CO2 absorption technique
  8. Targeting 5α-reductase with 99mTc labeled dutasteride derivatives for prostate imaging
  9. Changes in structural and optical properties due to γ-irradiation of MgO nanoparticles
  10. Radiation-induced free radicals from different milk powders and its possible use as radiation dosimeters
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Winner of the OpenAthens UX Award 2026
Winner of the OpenAthens UX Award 2026
Have an idea on how to improve our website?
Please write us.
© 2026 De Gruyter Brill
Downloaded on 11.3.2026 from https://www.degruyterbrill.com/document/doi/10.1515/ract-2019-3150/html
Scroll to top button